Exam 34: Applications of Flow Cytometry to Hematology and Hemostasis

In HIV infection, what is monitored to determine if antiviral treatment should be started?

______________ occurs when a chemical absorbs light, causing its electrons to be excited briefly to a higher, less-stable energy state.

Cell suspensions prepared from lymphoid tissues should be processed quickly to avoid artifacts caused by _____________________.

Residual white blood cells are detected in red blood cell packs by flow cytometry based on the presence of:

Which of the following is a software feature that allows the operator to focus on a single population of cells?

The specimen of choice for analysis of cell function by flow cytometry is:

In general, the greater the side-scattered (SSC) signal:

The dyes utilized by flow cytometry instruments include:

While evaluating data from an analysis, the analyst suspects spectral bleed-over has occurred. What can be done so that each detector only measures the signal for the appropriate fluorescent dye?

Which collection tube is used for enumeration of leukocyte subpopulations for blood and bone marrow?

Which of the following is an abnormal result for lymphocyte subset percentage in adult donors?

The forward-scattered (FSC) and side-scattered (SSC) values for lymphocytes compared with those for monocytes and neutrophils are:

Immunophenotyping is a staining approach that uses antibodies chemically linked to fluorochromes to recognize cell types by identifying specific ______________ on the surface of the cell or inside the cell.

Flow cytometry is a technology that provides rapid measurement of:

SSC is related to what characteristic of the blood cell?

Which of the following surface markers is specific for progenitor cells?

Malignant cells tend to have abnormal amounts of DNA. This is termed __________.

In DNA analysis by flow cytometry, better quantitation of fluorescence is achieved by measuring:

In flow cytometry, forward-angle light scatter provides information relevant to cell __________________.

In DNA analysis using flow cytometry, what type of signal is expected in cells with large amounts of DNA?