Question 1

The MPV is equivalent to the MCV and is __________ proportional to the platelet count in the Coulter S Plus IV platelet histogram.

Accepted Answer

A)  Directly 
B)  Indirectly 
C)  Inversely 
D)  All of the above 
E)  None of the above 
A)  Directly 
B)  Indirectly 
C)  Inversely 
D)  All of the above 
E)  None of the above C

Question 2

In the Coulter WBC differential technology, volume conductivity scattering (VCS) technology is particularly useful for identifying __________.

Accepted Answer

A)  Myelocytes 
B)  Eosinophils 
C)  Lymphocytes 
D)  Plasma cells 
E)  None of the above 
A)  Myelocytes 
B)  Eosinophils 
C)  Lymphocytes 
D)  Plasma cells 
E)  None of the above B

Question 3

The Technicon H1 instrument identifies and grades (1+ to 4+) various RBC abnormalities including:

Accepted Answer

A)  Microcytosis 
B)  Macrocytosis 
C)  Hypochromia 
D)  Hyperchromia 
E)  All of the above 
A)  Microcytosis 
B)  Macrocytosis 
C)  Hypochromia 
D)  Hyperchromia 
E)  All of the above E

Question 4

Applications of cell volume analysis in the Coulter S Plus IV differential provide information about which cell type?

Accepted Answer

A)  Granulocytes 
B)  Lymphocytes 
C)  Mononuclear cell fraction 
D)  Erythrocytes 
E)  None of the above 
A)  Granulocytes 
B)  Lymphocytes 
C)  Mononuclear cell fraction 
D)  Erythrocytes 
E)  None of the above

Question 5

An example of a WBC count measured by automated methods that would require a manual differential by certain laboratories is:

Accepted Answer

A)  9/L 
B)  5.5-10.0 F1F1F1S1F1F1F10 109/L 
C)  >15.0 F1F1F1S1F1F1F10 109/L 
D)  A, B, and C 
E)  A and C 
A)  9/L 
B)  5.5-10.0 F1F1F1S1F1F1F10 109/L 
C)  >15.0 F1F1F1S1F1F1F10 109/L 
D)  A, B, and C 
E)  A and C

Question 6

In the platelet histogram analysis by the Coulter S Plus IV automated instrument, particles with volume in the range of __________ are counted.

Accepted Answer

A)  5-10 fL 
B)  10-20 fL 
C)  2-20 fL 
D)  20-30 fL 
E)  >30 fL 
A)  5-10 fL 
B)  10-20 fL 
C)  2-20 fL 
D)  20-30 fL 
E)  >30 fL

Question 7

In the Coulter S Plus IV differential analysis of WBCs, those cells with volume ranging from 160 to 450 fL are classified as __________.

Accepted Answer

A)  Lymphocytes 
B)  Granulocytes 
C)  Mononuclear cells 
D)  All of the above 
E)  None of the above 
A)  Lymphocytes 
B)  Granulocytes 
C)  Mononuclear cells 
D)  All of the above 
E)  None of the above

Question 8

What does "backlighting" refer to in the Coulter S Plus IV instrument?

Accepted Answer

A)  Out-of-range results 
B)  The presence of abnormal cells or interferences 
C)  The possibility of erroneous or abnormal results 
D)  All of the above 
E)  None of the above 
A)  Out-of-range results 
B)  The presence of abnormal cells or interferences 
C)  The possibility of erroneous or abnormal results 
D)  All of the above 
E)  None of the above

Question 9

Where would an R3 (region 3) flag be located on a Coulter S Plus IV WBC histogram?

Accepted Answer

A)  Far left 
B)  Between lymphocytes and monocytes 
C)  Between monocytes and granulocytes 
D)  Far right 
E)  None of the above 
A)  Far left 
B)  Between lymphocytes and monocytes 
C)  Between monocytes and granulocytes 
D)  Far right 
E)  None of the above

Question 10

The Technicon methodology for differential cell analysis differs from the Coulter S Plus IV methodology in what respect?

Accepted Answer

A)  Cell volume 
B)  Light scatter 
C)  Cytochemistry 
D)  All of the above 
E)  None of the above 
A)  Cell volume 
B)  Light scatter 
C)  Cytochemistry 
D)  All of the above 
E)  None of the above

Question 11

Enumeration and identification of blood cells by the Technicon H1 automated blood analyzer is based on optical __________.

Accepted Answer

A)  Flow cytometry 
B)  Cytochemistry 
C)  Light scatter 
D)  All of the above 
E)  None of the above 
A)  Flow cytometry 
B)  Cytochemistry 
C)  Light scatter 
D)  All of the above 
E)  None of the above

Question 12

Conductivity measurements in Coulter VCS technology aid in differentiating between cells that are similar in __________.

Accepted Answer

A)  Size 
B)  Shape 
C)  Hemoglobin content 
D)  Iron content 
E)  None of the above 
A)  Size 
B)  Shape 
C)  Hemoglobin content 
D)  Iron content 
E)  None of the above

Question 13

The RDW is calculated as the coefficient of variation (CV) of the __________.

Accepted Answer

A)  Mean corpuscular volume (MCV) 
B)  Mean corpuscular hemoglobin (MCH) 
C)  Mean corpuscular hemoglobin concentration (MCH
C)  
D)  Mean platelet volume (MPV) 
E)  None of the above 
A)  Mean corpuscular volume (MCV) 
B)  Mean corpuscular hemoglobin (MCH) 
C)  Mean corpuscular hemoglobin concentration (MCH
C)  
D)  Mean platelet volume (MPV) 
E)  None of the above

Question 14

In Technicon H1 technology, red cells and platelets are counted based on their __________ properties.

Accepted Answer

A)  Conductance 
B)  Light scattering 
C)  Cell volume 
D)  Ion exchange 
E)  None of the above 
A)  Conductance 
B)  Light scattering 
C)  Cell volume 
D)  Ion exchange 
E)  None of the above

Question 15

An R1 (region 1) flag on the Coulter S Plus IV instrument represents the far left of the WBC histogram and is an indication of which possible abnormality?

Accepted Answer

A)  Cryoglobulins 
B)  Blasts 
C)  Eosinophilia 
D)  Immature granulocytes 
E)  None of the above 
A)  Cryoglobulins 
B)  Blasts 
C)  Eosinophilia 
D)  Immature granulocytes 
E)  None of the above

Question 16

Particles with a volume of greater than 36 fL and less than or equal to 360 fL are identified as __________ on a Coulter S Plus IV histogram analysis.

Accepted Answer

A)  Granulocytes 
B)  Red blood cells 
C)  Mononuclear cells 
D)  Lymphocytes 
E)  All of the above 
A)  Granulocytes 
B)  Red blood cells 
C)  Mononuclear cells 
D)  Lymphocytes 
E)  All of the above

Question 17

In the Technicon H1, cells are counted as they pass in front of a laser beam by a __________ detector.

Accepted Answer

A)  Cell volume 
B)  Conductivity 
C)  Light scatter 
D)  Ion 
E)  None of the above 
A)  Cell volume 
B)  Conductivity 
C)  Light scatter 
D)  Ion 
E)  None of the above

Question 18

The Coulter S Plus IV differential of granulocytes by cell volume consists of:

Accepted Answer

A)  Metamyelocytes 
B)  Segmented neutrophils 
C)  Bands 
D)  Eosinophils 
E)  All of the above 
A)  Metamyelocytes 
B)  Segmented neutrophils 
C)  Bands 
D)  Eosinophils 
E)  All of the above

Question 19

In the Technicon H1, leukocyte analysis involves staining with peroxidase in a heated chamber with lysis of platelets and __________.

Accepted Answer

A)  RBCs 
B)  WBCs 
C)  Mononuclear cells 
D)  All of the above 
E)  None of the above 
A)  RBCs 
B)  WBCs 
C)  Mononuclear cells 
D)  All of the above 
E)  None of the above

Question 20

The platelet distribution width (PDW) measures uniformity of platelet size and is equivalent to the __________ for red cells.

Accepted Answer

A)  MCV 
B)  RDW 
C)  MCHC 
D)  MCH 
E)  None of the above 
A)  MCV 
B)  RDW 
C)  MCHC 
D)  MCH 
E)  None of the above

Exam 32: Principles of Automated Differential Analysis

The MPV is equivalent to the MCV and is __________ proportional to the platelet count in the Coulter S Plus IV platelet histogram.

In the Coulter WBC differential technology, volume conductivity scattering (VCS) technology is particularly useful for identifying __________.

The Technicon H1 instrument identifies and grades (1+ to 4+) various RBC abnormalities including:

Applications of cell volume analysis in the Coulter S Plus IV differential provide information about which cell type?

An example of a WBC count measured by automated methods that would require a manual differential by certain laboratories is:

In the platelet histogram analysis by the Coulter S Plus IV automated instrument, particles with volume in the range of __________ are counted.

In the Coulter S Plus IV differential analysis of WBCs, those cells with volume ranging from 160 to 450 fL are classified as __________.

What does "backlighting" refer to in the Coulter S Plus IV instrument?

Where would an R3 (region 3) flag be located on a Coulter S Plus IV WBC histogram?

The Technicon methodology for differential cell analysis differs from the Coulter S Plus IV methodology in what respect?

Enumeration and identification of blood cells by the Technicon H1 automated blood analyzer is based on optical __________.

Conductivity measurements in Coulter VCS technology aid in differentiating between cells that are similar in __________.

The RDW is calculated as the coefficient of variation (CV) of the __________.

In Technicon H1 technology, red cells and platelets are counted based on their __________ properties.

An R1 (region 1) flag on the Coulter S Plus IV instrument represents the far left of the WBC histogram and is an indication of which possible abnormality?

Particles with a volume of greater than 36 fL and less than or equal to 360 fL are identified as __________ on a Coulter S Plus IV histogram analysis.

In the Technicon H1, cells are counted as they pass in front of a laser beam by a __________ detector.

The Coulter S Plus IV differential of granulocytes by cell volume consists of:

In the Technicon H1, leukocyte analysis involves staining with peroxidase in a heated chamber with lysis of platelets and __________.

The platelet distribution width (PDW) measures uniformity of platelet size and is equivalent to the __________ for red cells.

Morphology of Human Blood and Marrow Cells: Hematopoiesis

Bone Marrow

The Red Blood Cell: Structure and Function

Anemia: Diagnosis and Clinical Considerations

Evaluation of Red Blood Cell Morphology and Introduction to Platelet and White Blood Cell Morphology

Iron Metabolism and Hypochromic Anemias

Megaloblastic Anemias

Aplastic Anemia Including Pure Red Cell Aplasia and Congenital Dyserythropoietic Anemia and Paroxysmal Nocturnal Hemoglobinuria

Introduction to Hemolytic Anemias: Intracorpuscular Defects: I Hereditary Defects of the Red Cell Membrane

Hemolytic Anemias: Intracorpuscular Defects: II Hereditary Enzyme Deficiencies

Hemolytic Anemias: Intracorpuscular Defects: III the Hemoglobinopathies

Hemolytic Anemias: Intracorpuscular Defects: Iv Thalassemia

Hemolytic Anemias: Extracorpuscular Defects

Hypoproliferative Anemia: Anemia Associated With Systemic Diseases

Cell Biology, Disorders of Neutrophils, Infectious Mononucleosis, and Reactive Lymphocytosis

Introduction to Leukemia and the Acute Leukemias

Chronic Myeloproliferative Disorders I: Chronic Myelogenous Leukemia

Chronic Myeloproliferative Disorders Ii: Polycythemia Vera, Essential Thrombocythemia, and Idiopathic Myelofibrosis

Myelodysplastic Syndromes

Chronic Lymphocytic Leukemia and Related Lymphoproliferative Disorders

The Lymphomas

Multiple Myeloma and Related Plasma Cell Disorders

Lipid Lysosomal Storage Diseases and Histiocytosis

Introduction to Hemostasis

Disorders of Primary Hemostasis: Quantitative and Qualitative Platelet Disorders and Vascular Disorders

Disorders of Plasma Clotting Factors

Interaction of the Fibrinolytic, Coagulation, and Kinin Systems; Disseminated Intravascular Coagulation; and Related Pathology

Introduction to Thrombosis and Anticoagulant Therapy

Quality Control in the Hematology Laboratory

Body Fluid Examination: the Qualitative, Quantitative, and Morphologic Analysis of Serous, Cerebrospinal, and Synovial Fluids

Hematology Methods

Coagulation Procedures

Applications of Flow Cytometry to Hematology and Hemostasis

Molecular Diagnostic Techniques in Hematopathology

Special Stainscytochemistry

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