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Using an Antibody That Recognizes the Phosphorylated, but Not the Non-Phosphorylated

Question 17

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Using an antibody that recognizes the phosphorylated, but not the non-phosphorylated form of the transcription factor, NFAT, T cells are permeabilized, stained with this antibody, and analyzed by flow cytometry. Which of the data in Figure represent the expected pattern of staining from wild-type T cells before and after TCR stimulation. Using an antibody that recognizes the phosphorylated, but not the non-phosphorylated form of the transcription factor, NFAT, T cells are permeabilized, stained with this antibody, and analyzed by flow cytometry. Which of the data in Figure represent the expected pattern of staining from wild-type T cells before and after TCR stimulation.

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