Question 1

The excision (splicing)of many group I introns requires,in addition to the primary transcript RNA:

Accepted Answer

A) a cytosine nucleoside or nucleotide and a protein enzyme. 
B) a guanine nucleoside or nucleotide (only). 
C) a protein enzyme only. 
D) a small nuclear RNA and a protein enzyme. 
E) ATP,NAD,and a protein enzyme. 
A) a cytosine nucleoside or nucleotide and a protein enzyme. 
B) a guanine nucleoside or nucleotide (only). 
C) a protein enzyme only. 
D) a small nuclear RNA and a protein enzyme. 
E) ATP,NAD,and a protein enzyme.

Question 2

Splicing of introns in nuclear mRNA primary transcripts requires:

Accepted Answer

A) a guanine nucleoside or nucleotide. 
B) endoribonucleases. 
C) polynucleotide phosphorylase. 
D) RNA polymerase II. 
E) small nuclear ribonucleoproteins (snRNPs). 
A) a guanine nucleoside or nucleotide. 
B) endoribonucleases. 
C) polynucleotide phosphorylase. 
D) RNA polymerase II. 
E) small nuclear ribonucleoproteins (snRNPs).

Question 3

Below,an RNA molecule is being transcribed from a strand of DNA.Indicate the 5' and 3' ends of the RNA molecule and of the strand of DNA that is complementary to the RNA molecule.In which direction is synthesis occurring?

Accepted Answer

The answer of Below,an RNA molecule is being transcribed from...

Question 4

List one basic property that distinguishes RNA polymerases from DNA polymerases,and list one basic property they share.

Accepted Answer

Among the distinguishing characteristics

Question 5

In a $\rho$-independent terminator,there is a palindrome rich in G$ \equiv$C base pairs,followed by 8-10 uridine residues.Explain how each of the following changes might affect terminator function: 
(a)substitution of cytidines for the 8-10 uridines; 
(b)mutations in the palindrome that decrease its G$ \equiv$C content; 
(c)elimination of half of the palindromic sequence.

Accepted Answer

(a)This substitution would decrease term

Question 6

Which of the following is not a post-transcriptional modification in mRNA or tRNA?

Accepted Answer

A) 4-thiouridine 
B) Pseudouridine 
C) Dihydrouridine 
D) Ribouridine 
E) Ribothymidine 
A) 4-thiouridine 
B) Pseudouridine 
C) Dihydrouridine 
D) Ribouridine 
E) Ribothymidine

Question 7

Which of the following is not part of miRNA formation?

Accepted Answer

A) Pri-miRNA 
B) Pre-miRNA 
C) Dicer 
D) RISC 
E) Pseudouridine 
A) Pri-miRNA 
B) Pre-miRNA 
C) Dicer 
D) RISC 
E) Pseudouridine

Question 8

A branched ("lariat")structure is formed during:

Accepted Answer

A) attachment of a 5' cap to mRNA. 
B) attachment of poly(
A) tails to mRNA. 
C) processing of preribosomal RNA. 
D) splicing of all classes of introns. 
E) splicing of group II introns. 
A) attachment of a 5' cap to mRNA. 
B) attachment of poly(
A) tails to mRNA. 
C) processing of preribosomal RNA. 
D) splicing of all classes of introns. 
E) splicing of group II introns.

Question 9

"Footprinting," or DNase protection,is a technique used to identify:

Accepted Answer

A) a region of DNA that has been damaged by mutation. 
B) 
E) coli cells that contain a desired,cloned piece of DNA. 
C) the position of a particular gene of a chromosome. 
D) the position of internally double-stranded regions in a single-stranded DNA molecule. 
E) the specific binding site of a repressor,polymerase,or other protein on the DNA. 
A) a region of DNA that has been damaged by mutation. 
B) 
E) coli cells that contain a desired,cloned piece of DNA. 
C) the position of a particular gene of a chromosome. 
D) the position of internally double-stranded regions in a single-stranded DNA molecule. 
E) the specific binding site of a repressor,polymerase,or other protein on the DNA.

Question 10

RNA polymerase from E.coli (core enzyme alone)has all of the following properties except that it:

Accepted Answer

A) can extend an RNA chain and initiate a new chain. 
B) is required for the synthesis of mRNA,rRNA,and tRNA in E.coli. 
C) produces an RNA polymer that begins with a 5'-triphosphate. 
D) recognizes specific start signals in DNA. 
E) requires all four ribonucleoside triphosphates and a DNA template. 
A) can extend an RNA chain and initiate a new chain. 
B) is required for the synthesis of mRNA,rRNA,and tRNA in E.coli. 
C) produces an RNA polymer that begins with a 5'-triphosphate. 
D) recognizes specific start signals in DNA. 
E) requires all four ribonucleoside triphosphates and a DNA template.

Question 11

After binding by E.coli RNA polymerase,the correct order of events for transcription initiation is:

Accepted Answer

A) closed complex formation,open complex formation,promoter clearance,start of RNA synthesis. 
B) closed complex formation,open complex formation,start of RNA synthesis,promoter clearance. 
C) open complex formation,closed complex formation,start of RNA synthesis,promoter clearance. 
D) start of RNA synthesis,closed complex formation,open complex formation,promoter clearance. 
E) start of RNA synthesis,open complex formation,closed complex formation,promoter clearance. 
A) closed complex formation,open complex formation,promoter clearance,start of RNA synthesis. 
B) closed complex formation,open complex formation,start of RNA synthesis,promoter clearance. 
C) open complex formation,closed complex formation,start of RNA synthesis,promoter clearance. 
D) start of RNA synthesis,closed complex formation,open complex formation,promoter clearance. 
E) start of RNA synthesis,open complex formation,closed complex formation,promoter clearance.

Question 12

Describe the sequence of events in the initiation of transcription by E.coli RNA polymerase.

Accepted Answer

The core enzyme plus @#LAT-DLM& subunit,called hol

Question 13

Define ribozymes and briefly describe the structure and function of two ribozymes.

Accepted Answer

Ribozymes are enzymes that consist in pa

Question 14

Describe briefly the process of initiation by eukaryotic RNA polymerase.

Accepted Answer

One transcription factor (TBP)binds spec

Question 15

Which of these polymerases does not require a template?

Accepted Answer

A) RNA pol I 
B) RNA pol II 
C) Reverse transcriptase 
D) Polyadenylate polymerase 
E) RNA replicase 
A) RNA pol I 
B) RNA pol II 
C) Reverse transcriptase 
D) Polyadenylate polymerase 
E) RNA replicase

Question 16

In 1989,Sidney Altman won a Nobel Prize for his work on RNase P.In no more than three sentences,describe the function of RNase P,as well as the unusual characteristic of this enzyme that made Altman's work so important.

Accepted Answer

RNase P catalyzes the removal

Question 17

Describe the mechanistic difference that distinguishes the splicing of group I introns from that of group II introns.

Accepted Answer

In the group I introns,the initial break

Question 18

Which of the following is not usually essential for the catalytic activity of ribozymes?

Accepted Answer

A) Correct base pairing 
B) Correct base sequence 
C) Correct interaction with protein 
D) Correct secondary structure 
E) Correct three-dimensional structure 
A) Correct base pairing 
B) Correct base sequence 
C) Correct interaction with protein 
D) Correct secondary structure 
E) Correct three-dimensional structure

Exam 26: Rna Metabolism

The excision (splicing)of many group I introns requires,in addition to the primary transcript RNA:

Splicing of introns in nuclear mRNA primary transcripts requires:

Below,an RNA molecule is being transcribed from a strand of DNA.Indicate the 5' and 3' ends of the RNA molecule and of the strand of DNA that is complementary to the RNA molecule.In which direction is synthesis occurring?

List one basic property that distinguishes RNA polymerases from DNA polymerases,and list one basic property they share.

Which of the following is not a post-transcriptional modification in mRNA or tRNA?

Which of the following is not part of miRNA formation?

A branched ("lariat")structure is formed during:

"Footprinting," or DNase protection,is a technique used to identify:

RNA polymerase from E.coli (core enzyme alone)has all of the following properties except that it:

After binding by E.coli RNA polymerase,the correct order of events for transcription initiation is:

Describe the sequence of events in the initiation of transcription by E.coli RNA polymerase.

Define ribozymes and briefly describe the structure and function of two ribozymes.

Describe briefly the process of initiation by eukaryotic RNA polymerase.

Which of these polymerases does not require a template?

In 1989,Sidney Altman won a Nobel Prize for his work on RNase P.In no more than three sentences,describe the function of RNase P,as well as the unusual characteristic of this enzyme that made Altman's work so important.

Describe the mechanistic difference that distinguishes the splicing of group I introns from that of group II introns.

Which of the following is not usually essential for the catalytic activity of ribozymes?

The Foundations of Biochemistry

Water

Amino Acids, peptides, and Proteins

The Three-Dimensional Structure of Proteins

Protein Function

Enzymes

Carbohydrates and Glycobiology

Nucleotides and Nucleic Acids

Recombinant Dna Technology

Lipids

Biological Membranes and Transport

Biosignaling

Principles of Bioenergetics

Glycolysis, gluconeogenesis, and the Pentose Phosphate Pathway

Principles of Metabolic Regulation

The Citric Acid Cycle

Fatty Acid Catabolism

Amino Acid Oxidation and the Production of Urea

Oxidative Phosphorylation and Photophosphorylation

Carbohydrate Biosynthesis in Plants and Bacteria

Lipid Biosynthesis

Biosynthesis of Amino Acids, nucleotides, and Related Molecules

Integration and Hormonal Regulation of Mammalian Metabolism

Genes and Chromosomes

Dna Metabolism

Protein Metabolism

Regulation of Gene Expression

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