Exam 9: Biochemical Identification of Gram-Negative Bacteria

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These bacteria are unable to ferment either lactose or glucose, but they can degrade the peptones present in the TSI agar or KIA aerobically or anaerobically, resulting in the production of alkaline by-products in the slant or deep, respectively, changing the indicator to a deep red color. What kind of bacteria cannot ferment lactose or glucose?

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The decarboxylase tests determine

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The medium used in the decarboxylase test is called

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Biochemical tests are based on the _____ of microorganisms.

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In the Voges-Proskauer test, you are testing for the metabolic by-products of which pathway?

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Some bacteria are asaccharolytic. This means that these bacteria

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Oxidative/fermentative (O/F) basal medium is a medium that will test the oxidative and fermentative capabilities of a microbe. The pH indicator is _____.

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Using Moeller's decarboxylase base medium, what color is a positive reaction and what causes this color to be produced?

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If an organism fermented glucose only and utilized peptones, what would the TSI reaction be?

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Commercial identification systems fall into all of the following categories, except

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