Question 1

Within what time after collection of whole blood must RBCs be separated from whole blood in order for platelet and plasma components to be prepared?

Accepted Answer

A)  Immediately 
B)  2 to 5 hours 
C)  8 to 24 hours 
D)  Any time 
A)  Immediately 
B)  2 to 5 hours 
C)  8 to 24 hours 
D)  Any time

Question 2

What dose of RhIG would be appropriate for a D-negative woman who has had a miscarriage at 11 weeks' gestation?

Accepted Answer

A)  300 µg 
B)  120 µg 
C)  50 µg 
D)  All of the above 
A)  300 µg 
B)  120 µg 
C)  50 µg 
D)  All of the above

Question 3

A unit of whole blood must be stored at what temperature?

Accepted Answer

A)  -65° to -20°C 
B)  - 10° to 2°C 
C)  1° to 6°C 
D)  8° to 20°C 
A)  -65° to -20°C 
B)  - 10° to 2°C 
C)  1° to 6°C 
D)  8° to 20°C

Question 4

What is the shelf life of whole blood collected in citrate-phosphate-dextrose-adenine?

Accepted Answer

A)  10 days 
B)  21 days 
C)  35 days 
D)  50 days 
A)  10 days 
B)  21 days 
C)  35 days 
D)  50 days

Question 5

Which factor concentrate has almost completely replaced cryoprecipitate as the product of choice to treat patients with hemophilia A?

Accepted Answer

A)  FVIIa 
B)  rFVIIa 
C)  FVIII 
D)  FVIX 
A)  FVIIa 
B)  rFVIIa 
C)  FVIII 
D)  FVIX

Question 6

How is the preparation of PPF different than that of NSA?

Accepted Answer

A)  PPF is salvaged and pooled; NSA is not pooled. 
B)  PPF is cold ethanol fractionated; NSA is warm alcohol fractionated. 
C)  PPF uses heat inactivation of viruses; NSA does not. 
D)  PPF contains less albumin and more globulins than NSA. 
A)  PPF is salvaged and pooled; NSA is not pooled. 
B)  PPF is cold ethanol fractionated; NSA is warm alcohol fractionated. 
C)  PPF uses heat inactivation of viruses; NSA does not. 
D)  PPF contains less albumin and more globulins than NSA.

Question 7

A unit of packed platelets must be stored at what temperature?

Accepted Answer

A)  1° to 6°C 
B)  8° to 20°C 
C)  20° to 24°C 
D)  32° to 45°C 
A)  1° to 6°C 
B)  8° to 20°C 
C)  20° to 24°C 
D)  32° to 45°C

Question 8

What is the storage temperature for normal serum albumin?

Accepted Answer

A)  -80° to -65°C 
B)  -20° to 0°C 
C)  2° to 10°C 
D)  20° to 25°C 
A)  -80° to -65°C 
B)  -20° to 0°C 
C)  2° to 10°C 
D)  20° to 25°C

Question 9

In which animal has transgenic methods produced rAT in the milk?

Accepted Answer

A)  Cows 
B)  Goats 
C)  Pigs 
D)  Sheep 
A)  Cows 
B)  Goats 
C)  Pigs 
D)  Sheep

Question 10

Viruses are inactivated in units of plasma factor concentrate by all of these methods EXCEPT:

Accepted Answer

A)  freezing. 
B)  heating. 
C)  solvent-detergent. 
D)  nanofiltration. 
A)  freezing. 
B)  heating. 
C)  solvent-detergent. 
D)  nanofiltration.

Question 11

Which statement about frozen plasma is correct?

Accepted Answer

A)  The expiration of frozen plasma stored at -18°C is 10 years. 
B)  The expiration of frozen plasma stored at -65°C is 7 years. 
C)  The expiration of frozen plasma stored at -10° to 0°C is 5 years. 
D)  All of these are correct. 
A)  The expiration of frozen plasma stored at -18°C is 10 years. 
B)  The expiration of frozen plasma stored at -65°C is 7 years. 
C)  The expiration of frozen plasma stored at -10° to 0°C is 5 years. 
D)  All of these are correct.

Question 12

What is the storage temperature for plasma protein fraction?

Accepted Answer

A)  -80° to -65°C 
B)  -20° to 0°C 
C)  2° to 10°C 
D)  20° to 25°C 
A)  -80° to -65°C 
B)  -20° to 0°C 
C)  2° to 10°C 
D)  20° to 25°C

Question 13

Which of the following statements explains why bacterial contamination of blood is rarely a problem?

Accepted Answer

A)  Regulated procedures for arm preparation prevent most bacterial contamination from occurring during collection. 
B)  Citrate in the anticoagulant is not conducive to bacterial growth. 
C)  The cold storage of blood is not conducive to bacterial growth. 
D)  All of the above 
A)  Regulated procedures for arm preparation prevent most bacterial contamination from occurring during collection. 
B)  Citrate in the anticoagulant is not conducive to bacterial growth. 
C)  The cold storage of blood is not conducive to bacterial growth. 
D)  All of the above

Question 14

Leukoreduced packed RBCs must have an absolute white blood cell count of less than and contain at least what percent of original RBC mass?

Accepted Answer

A)  5 $\times$ 108/85 
B)  5 $\times$ 106/85 
C)  5 $\times$ 108/80 
D)  5 $\times$ 106/80 
A)  5 $\times$ 108/85 
B)  5 $\times$ 106/85 
C)  5 $\times$ 108/80 
D)  5 $\times$ 106/80

Question 15

Which of the follow is NOT considered in the preparation of Rho immunoglobulin?

Accepted Answer

A)  Donors are Rh-(
D)  positive. 
B)  Donors have been hyperimmunized. 
C)  Donor plasmas contain IgG anti-D. 
D)  Donor human plasmas are pooled. 
A)  Donors are Rh-(
D)  positive. 
B)  Donors have been hyperimmunized. 
C)  Donor plasmas contain IgG anti-D. 
D)  Donor human plasmas are pooled.

Question 16

Xenographic forms of factor VIII are made from which source of plasma?

Accepted Answer

A)  Bovine plasma 
B)  Human plasma 
C)  Porcine plasma 
D)  Reptilian plasma 
A)  Bovine plasma 
B)  Human plasma 
C)  Porcine plasma 
D)  Reptilian plasma

Question 17

Packed RBCs must have a final hematocrit of less than or equal to:

Accepted Answer

A)  85%. 
B)  38%. 
C)  80%. 
D)  70%. 
A)  85%. 
B)  38%. 
C)  80%. 
D)  70%.

Question 18

A unit of granulocyte should contain at least what concentration of granulocytes?

Accepted Answer

A)  1  1010 
B)  4  1010 
C)  6  1010 
D)  10  1010 
A)  1  1010 
B)  4  1010 
C)  6  1010 
D)  10  1010

Question 19

What is the expiration date of a deglycerolized unit of RBCs?

Accepted Answer

A)  10 hours 
B)  24 hours 
C)  36 hours 
D)  72 hours 
A)  10 hours 
B)  24 hours 
C)  36 hours 
D)  72 hours

Question 20

What is the shelf-life for normal serum albumin?

Accepted Answer

A)  1 year 
B)  3 years 
C)  5 years 
D)  10 years 
A)  1 year 
B)  3 years 
C)  5 years 
D)  10 years

Exam 15: Component Preparation

Within what time after collection of whole blood must RBCs be separated from whole blood in order for platelet and plasma components to be prepared?

What dose of RhIG would be appropriate for a D-negative woman who has had a miscarriage at 11 weeks' gestation?

A unit of whole blood must be stored at what temperature?

What is the shelf life of whole blood collected in citrate-phosphate-dextrose-adenine?

Which factor concentrate has almost completely replaced cryoprecipitate as the product of choice to treat patients with hemophilia A?

How is the preparation of PPF different than that of NSA?

A unit of packed platelets must be stored at what temperature?

What is the storage temperature for normal serum albumin?

In which animal has transgenic methods produced rAT in the milk?

Viruses are inactivated in units of plasma factor concentrate by all of these methods EXCEPT:

Which statement about frozen plasma is correct?

What is the storage temperature for plasma protein fraction?

Which of the following statements explains why bacterial contamination of blood is rarely a problem?

Leukoreduced packed RBCs must have an absolute white blood cell count of less than and contain at least what percent of original RBC mass?

Which of the follow is NOT considered in the preparation of Rho immunoglobulin?

Xenographic forms of factor VIII are made from which source of plasma?

Packed RBCs must have a final hematocrit of less than or equal to:

A unit of granulocyte should contain at least what concentration of granulocytes?

What is the expiration date of a deglycerolized unit of RBCs?

What is the shelf-life for normal serum albumin?

Red Blood Cells and Platelet Preservation: Historical Perspectives and Current Trends

Basic Genetics

Fundamentals of Immunology

Concepts in Molecular Biology

The Antiglobulin Test

The Abo Blood Group System

The Rh Blood Group System

Blood Group Terminology and Common Blood Groups

Uncommon Blood Groups

Detection and Identification of Antibodies

Pre-Transfusion Testing

Blood Bank Technologies and Automation

Donor Selection

Transfusion-Transmitted Diseases

Transfusion Therapy

Adverse Effects of Blood Transfusion

Apheresis

Cellular Therapy in the Transplant Setting

Hemolytic Disease of the Fetus and Newborn

Autoimmune Hemolytic Anemias

Tissue Banking As Part of the Transfusion Service

The Hla System

Relationship Testing

Quality Management

Patient Blood Management

Transfusion Safety and Federal Regulatory Requirements

Laboratory Information Systems in the Blood Bank

Medicolegal and Ethical Aspects of Providing Blood Collection and Transfusion Services

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