Question 1

Which of the following statements about regulation of gene expression is CORRECT?

Accepted Answer

A)  An inducible gene is transcribed when a specific substance is absent. 
B)  A gene is any DNA sequence that is transcribed into an mRNA molecule only. 
C)  All genes are transcribed at all times as long as they have a functional promoter. 
D)  The regulation of gene expression is the same in both eukaryotes and prokaryotes. 
E)  The regulation of gene expression is critical for the control of life processes in all organisms. 
A)  An inducible gene is transcribed when a specific substance is absent. 
B)  A gene is any DNA sequence that is transcribed into an mRNA molecule only. 
C)  All genes are transcribed at all times as long as they have a functional promoter. 
D)  The regulation of gene expression is the same in both eukaryotes and prokaryotes. 
E)  The regulation of gene expression is critical for the control of life processes in all organisms.

Question 2

A partial diploid E. coli cell of lacI+ lacP- lacOc lacI+ lacY+ / lacIs lacP+lacO+ lacZ+ lacY- will synthesize:

Accepted Answer

A)  both lacZ and lacY functional gene products in the absence of lactose. 
B)  neither lacZ nor lacY functional gene products in the presence of lactose. 
C)  both lacZ and lacY functional gene products in the presence of lactose. 
D)  lacY but not a functional lacZ gene product in the absence of lactose. 
E)  lacZ but not a functional lacY gene product in the absence of lactose. 
A)  both lacZ and lacY functional gene products in the absence of lactose. 
B)  neither lacZ nor lacY functional gene products in the presence of lactose. 
C)  both lacZ and lacY functional gene products in the presence of lactose. 
D)  lacY but not a functional lacZ gene product in the absence of lactose. 
E)  lacZ but not a functional lacY gene product in the absence of lactose.

Question 3

Which of the following statements about DNA-binding proteins is NOT true?

Accepted Answer

A)  Specific amino acids within the motif form hydrogen bonds with DNA. 
B)  These proteins can affect the expression of a gene. 
C)  Most DNA-binding proteins bind dynamically. 
D)  Some of these proteins incorporate a metal ion such as zinc. 
E)  Once bound, most of DNA-binding proteins remain on DNA permanently. 
A)  Specific amino acids within the motif form hydrogen bonds with DNA. 
B)  These proteins can affect the expression of a gene. 
C)  Most DNA-binding proteins bind dynamically. 
D)  Some of these proteins incorporate a metal ion such as zinc. 
E)  Once bound, most of DNA-binding proteins remain on DNA permanently.

Question 4

Describe one similarity and one difference in how the trp and lac repressor proteins function.

Accepted Answer

One similarity between the trp and lac r

Question 5

An E. coli strain of chromosomal genotype lacI- lacP+ lacO+ lacZ+ lacY+ constitutively expresses the genes of the lac operon. The strain is converted to wild-type lac operon regulation by the addition of an extra piece of DNA. What gene or genes are contained on this extra DNA that explain this conversion to wild type? Include an explanation of cis- or trans-acting factors and how they work.

Accepted Answer

The extra piece of DNA contains the wild

Question 6

The formation of 1+2 and 3+4 secondary structures of 5' UTR region mRNA from the trp operon is triggered when:

Accepted Answer

A)  the tryptophan level inside the bacterial cell is extremely low. 
B)  the tryptophan level inside the bacterial cell is high. 
C)  the repressor protein fails to bind to the operator. 
D)  there is a spontaneous mutation introduced into the 5' UTR. 
E)  the structural gene transcription within the trp operon gets initiated. 
A)  the tryptophan level inside the bacterial cell is extremely low. 
B)  the tryptophan level inside the bacterial cell is high. 
C)  the repressor protein fails to bind to the operator. 
D)  there is a spontaneous mutation introduced into the 5' UTR. 
E)  the structural gene transcription within the trp operon gets initiated.

Question 7

Which of the following statements about gene regulation concerning operons is INCORRECT?

Accepted Answer

A)  A negative repressible gene is controlled by a regulatory protein that inhibits transcription. 
B)  For a gene under negative repressible control, a small molecule is required to prevent the gene's repressor from binding to DNA. 
C)  For a gene under positive repressible control, the normal state is transcription of a gene, stimulated by a transcriptional activator. 
D)  A regulator gene has its own promoter and is transcribed into an independent mRNA. 
E)  Presence of operons where genes of related functions are clustered is common in bacteria but not in eukaryotes. 
A)  A negative repressible gene is controlled by a regulatory protein that inhibits transcription. 
B)  For a gene under negative repressible control, a small molecule is required to prevent the gene's repressor from binding to DNA. 
C)  For a gene under positive repressible control, the normal state is transcription of a gene, stimulated by a transcriptional activator. 
D)  A regulator gene has its own promoter and is transcribed into an independent mRNA. 
E)  Presence of operons where genes of related functions are clustered is common in bacteria but not in eukaryotes.

Question 8

Fill in the blanks in the following table for the transcription status of the lac operon ((+) for transcription and (-) for no transcription) in the absence or presence of lactose. The first line is filled in for reference.

Accepted Answer

The answer of Fill in the blanks in the following...

Which of the following statements about regulation of gene expression is CORRECT?

A partial diploid E. coli cell of lacI⁺ lacP^-lacO^c lacI⁺lacY⁺ / lacI^s lacP⁺lacO⁺ lacZ⁺ lacY^- will synthesize:

Which of the following statements about DNA-binding proteins is NOT true?

Describe one similarity and one difference in how the trp and lac repressor proteins function.

The formation of 1+2 and 3+4 secondary structures of 5' UTR region mRNA from the trp operon is triggered when:

Which of the following statements about gene regulation concerning operons is INCORRECT?

Fill in the blanks in the following table for the transcription status of the lac operon ((+) for transcription and (-) for no transcription) in the absence or presence of lactose. The first line is filled in for reference.

Introduction to Genetics

Chromosomes and Cellular Reproduction

Basic Principles of Heredity

Sex Determination and Sex-Linked Characteristics

Extensions and Modifications of Basic Principles

Pedigree Analysis, Applications, and Genetic Testing

Linkage, Recombination, and Eukaryotic Gene Mapping

Chromosome Variation

Bacterial and Viral Genetic Systems

DNA: the Chemical Nature of the Gene

Chromosome Structure and Organelle Dna

DNA Replication and Recombination

Transcription

Rna Molecules and Rna Processing

The Genetic Code and Translation

Control of Gene Expression in Eukaryotes

Gene Mutations and Dna Repair

Molecular Genetic Analysis and Biotechnology

Genomics and Proteomics

Epigenetics

Developmental Genetics and Immunogenetics

Cancer Genetics

Quantitative Genetics

Population Genetics

Evolutionary Genetics

Filters

Exam 16: Control of Gene Expression in Prokaryotes

Which of the following statements about regulation of gene expression is CORRECT?

A partial diploid E. coli cell of lacI+ lacP- lacOc lacI+ lacY+ / lacIs lacP+lacO+ lacZ+ lacY- will synthesize:

Which of the following statements about DNA-binding proteins is NOT true?

Describe one similarity and one difference in how the trp and lac repressor proteins function.

The formation of 1+2 and 3+4 secondary structures of 5' UTR region mRNA from the trp operon is triggered when:

Which of the following statements about gene regulation concerning operons is INCORRECT?

Fill in the blanks in the following table for the transcription status of the lac operon ((+) for transcription and (-) for no transcription) in the absence or presence of lactose. The first line is filled in for reference.

Introduction to Genetics

Chromosomes and Cellular Reproduction

Basic Principles of Heredity

Sex Determination and Sex-Linked Characteristics

Extensions and Modifications of Basic Principles

Pedigree Analysis, Applications, and Genetic Testing

Linkage, Recombination, and Eukaryotic Gene Mapping

Chromosome Variation

Bacterial and Viral Genetic Systems

DNA: the Chemical Nature of the Gene

Chromosome Structure and Organelle Dna

DNA Replication and Recombination

Transcription

Rna Molecules and Rna Processing

The Genetic Code and Translation

Control of Gene Expression in Eukaryotes

Gene Mutations and Dna Repair

Molecular Genetic Analysis and Biotechnology

Genomics and Proteomics

Epigenetics

Developmental Genetics and Immunogenetics

Cancer Genetics

Quantitative Genetics

Population Genetics

Evolutionary Genetics

Filters

A partial diploid E. coli cell of lacI⁺ lacP^-lacO^c lacI⁺lacY⁺ / lacI^s lacP⁺lacO⁺ lacZ⁺ lacY^- will synthesize: