Exam 9: Controlling Microbial Growth in the Environment

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The lowest temperature that kills all cells in a broth in 10 minutes is known as the (microbial/thermal) death point.

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thermal

Which of the following statements about aldehydes is FALSE?

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B

A student is shopping for antibacterial hand cleansers and is trying to decide which one to buy. One is a "waterless" hand gel containing 70% isopropanol, the second is an "antibacterial" hand soap containing triclosan (a phenolic), and the third is a wipe that lists benzethonium chloride (a synthetic quaternary ammonium salt) as the active ingredient. Compare and contrast these cleansers in terms of the action of the antimicrobial ingredient and the level of disinfection (degerming, germistatic, germicidal).

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All three are germicidal, although not all to the same degree. The alcohol of the waterless hand cleaner is a germicide that disrupts cytoplasmic membranes and denatures proteins. It is not effective against bacterial endospores or fungal spores, and it has limited effect on nonenveloped viruses. The alcohol evaporates quickly, so the germicidal effect is short term. The waterless hand cleaner is an
intermediate- level disinfectant and is not an effective degermer. The hand soap contains a phenolic, which also damages cytoplasmic membranes and denatures proteins, and is effective on the same range of microbes as the alcohol. Phenolics are intermediate- level disinfectants that persist on surfaces for long periods of time, providing extended disinfection. If used with running water and the hands are vigorously rubbed, the hand soap can be an effective degermer. Synthetic "quats" (quaternary ammonium compounds) disrupt cell membranes. They are effective against fungi, enveloped viruses, and most bacteria, but not against nonenveloped viruses, nor on endospores. Quats are low- level disinfectants that are germicidal for some microbes. The action of using and discarding the wipe provides some degerming effect.

Elements such as iodine, chlorine, and bromine are examples of (oxidizers/halogens/metals), which are the basis for many effective antimicrobial agents.

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Seventy percent alcohol is effective against

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By themselves, soaps have only degerming activity, not antimicrobial activity.

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A(n) (iodophor/halogen) is an iodine- containing organic compound found in such antiseptics as Betadine.

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Ultraviolet light penetrates (more/less) effectively than gamma rays.

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The dairy creamer used in restaurants is usually treated by

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The process of freeze- drying microbes to preserve them is (lyophilization/dessication).

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Compare and contrast the nine major types of antimicrobial chemicals in terms of their effective on microbes.

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Disinfectants known as (alcohols/oxidizers/aldehydes) have the chemical group - CHO, which reacts with and damages both proteins and nucleic acids.

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The phenol coefficient is one of the most widely used measurements of an antimicrobial agent's effectiveness.

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Aseptic means

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Disinfectants that damage membranes include

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Some viruses are inactivated by the same chemical or physical agents that damage cytoplasmic membranes.

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A chemical agent that kills pathogenic microbes in general is a(n)

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Which of the following statements about quaternary ammonium compounds is FALSE?

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The deadliest, most contagious microbes are studied under conditions of (BSL- 4/BSL- 3/BSL- 2/BSL- 1) containment.

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Which of the following infectious agents is least resistant to destruction by chemical methods?

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