Question 1

A thin film of microbes on a slide is

Accepted Answer

A)  agglutination. 
B)  fixation. 
C)  a biopsy. 
D)  a smear. 
E)  a Gram reaction. 
A)  agglutination. 
B)  fixation. 
C)  a biopsy. 
D)  a smear. 
E)  a Gram reaction. D

Question 2

The endospore stain reveals internal structures within cells of the genera Bacillus and Clostridium.

Accepted Answer

A) True 
 B)False  True

Question 3

Safranin dye is used as the counterstain in stain(s).

Accepted Answer

A)  the endospore 
B)  the flagellar 
C)  the acid- fast 
D)  the Gram 
E)  both the Gram and the endospore 
A)  the endospore 
B)  the flagellar 
C)  the acid- fast 
D)  the Gram 
E)  both the Gram and the endospore E

Question 4

Coating a specimen with a heavy metal is a step in preparing it for (phase/fluorescent/electron) microscopy.

Accepted Answer

The answer of Coating a specimen with a heavy metal...

Question 5

The system of taxonomy used today was originated by (Linnaeus/Darwin/Woese). (Be sure to capitalize your answer.)

Accepted Answer

The answer of The system of taxonomy used today was...

Question 6

If a microbiology lab student left the safranin out of the Gram stain procedure, what would be the result?

Accepted Answer

A)  Gram- positive cells would be purple and Gram- negative cells would be colorless. 
B)  All cells would be purple. 
C)  Gram- positive cells would be pink and Gram- negative cells would be purple. 
D)  Gram- positive cells would be colorless and Gram- negative cells would be pink. 
E)  All cells would be pink. 
A)  Gram- positive cells would be purple and Gram- negative cells would be colorless. 
B)  All cells would be purple. 
C)  Gram- positive cells would be pink and Gram- negative cells would be purple. 
D)  Gram- positive cells would be colorless and Gram- negative cells would be pink. 
E)  All cells would be pink.

Question 7

The Gram stain works because of differences in the of bacteria.

Accepted Answer

A)  cell membranes 
B)  antigens 
C)  cell walls 
D)  capsules 
E)  genetic characteristics 
A)  cell membranes 
B)  antigens 
C)  cell walls 
D)  capsules 
E)  genetic characteristics

Question 8

A measurement of a microbe is reported as 1 × 10- 6 m, also known as

Accepted Answer

A)  millimeters (mm). 
B)  nanometers (nm). 
C)  yards. 
D)  centimeters (cm). 
E)  micrometers (µm). 
A)  millimeters (mm). 
B)  nanometers (nm). 
C)  yards. 
D)  centimeters (cm). 
E)  micrometers (µm).

Question 9

The size of a pork tapeworm is generally described in terms of

Accepted Answer

A)  decimeters (dc). 
B)  meters (m). 
C)  micrometers (µm). 
D)  nanometers (nm). 
E)  millimeters (mm.) 
A)  decimeters (dc). 
B)  meters (m). 
C)  micrometers (µm). 
D)  nanometers (nm). 
E)  millimeters (mm.)

Question 10

The primary goal of modern taxonomists is to

Accepted Answer

A)  understand the phylogenetic relationships between organisms. 
B)  determine what defines a species. 
C)  establish unique identifiers for each species. 
D)  have a complete inventory of all living things. 
E)  There is no consensus among taxonomists regarding their primary goal. 
A)  understand the phylogenetic relationships between organisms. 
B)  determine what defines a species. 
C)  establish unique identifiers for each species. 
D)  have a complete inventory of all living things. 
E)  There is no consensus among taxonomists regarding their primary goal.

Question 11

All of the following are common to both the Gram stain and the acid- fast stain EXCEPT

Accepted Answer

A)  counterstain. 
B)  primary stain. 
C)  a chemical mordant. 
D)  a decolorizing agent. 
E)  a decolorizing agent and a counterstain. 
A)  counterstain. 
B)  primary stain. 
C)  a chemical mordant. 
D)  a decolorizing agent. 
E)  a decolorizing agent and a counterstain.

Question 12

Which of the following classification methods relies on the morphology of organisms?

Accepted Answer

A)  analysis of nucleic acids 
B)  biochemical tests 
C)  phage typing 
D)  physical (phenotypic) characteristics 
E)  serological tests 
A)  analysis of nucleic acids 
B)  biochemical tests 
C)  phage typing 
D)  physical (phenotypic) characteristics 
E)  serological tests

Question 13

Figure 4- 1 represents a (dichotomous/taxonomic) key of the type used to identify a microbe.

Accepted Answer

The answer of   Figure 4- 1 represents a...

Question 14

A(n) (acidic/metallic/fluorescent) molecule is one that absorbs invisible radiation and emits visible light.

Accepted Answer

The answer of A(n) (acidic/metallic/fluorescent) molecule is one that absorbs...

Question 15

A primary purpose for the use of stains in microscopy is the increase the (magnification/brightness/contrast) of a specimen.

Accepted Answer

The answer of A primary purpose for the use of...

Question 16

The total magnification using a 10 ocular and a 100 objective would be (110/1000/10000) X. (Be sure your answer is a numeral.)

Accepted Answer

The answer of The total magnification using a 10 ocular...

Question 17

Which of the following statements about transmission electron microscopy is FALSE?

Accepted Answer

A)  Thin segments must be used. 
B)  Electrons are the source of visualization. 
C)  Glass lenses are used. 
D)  Stains can be applied to improve contrast. 
E)  The sample is placed in a vacuum. 
A)  Thin segments must be used. 
B)  Electrons are the source of visualization. 
C)  Glass lenses are used. 
D)  Stains can be applied to improve contrast. 
E)  The sample is placed in a vacuum.

Question 18

Carbolfuchsin is the _ in the acid- fast stain.

Accepted Answer

A)  primary stain 
B)  counterstain 
C)  fixing reagent 
D)  decolorizer 
E)  mordant 
A)  primary stain 
B)  counterstain 
C)  fixing reagent 
D)  decolorizer 
E)  mordant

Question 19

Tungsten is a reagent used in the

Accepted Answer

A)  flagellar stain. 
B)  negative stain. 
C)  acid- fast stain. 
D)  electron microscopy stain. 
E)  endospore stain. 
A)  flagellar stain. 
B)  negative stain. 
C)  acid- fast stain. 
D)  electron microscopy stain. 
E)  endospore stain.

Question 20

A staining procedure that uses a single basic dye is called a (simple/basic/cationic) stain.

Accepted Answer

The answer of A staining procedure that uses a single...

Exam 4: Microscopy, Staining, and Classification

A thin film of microbes on a slide is

The endospore stain reveals internal structures within cells of the genera Bacillus and Clostridium.

Safranin dye is used as the counterstain in stain(s).

Coating a specimen with a heavy metal is a step in preparing it for (phase/fluorescent/electron) microscopy.

The system of taxonomy used today was originated by (Linnaeus/Darwin/Woese). (Be sure to capitalize your answer.)

If a microbiology lab student left the safranin out of the Gram stain procedure, what would be the result?

The Gram stain works because of differences in the of bacteria.

A measurement of a microbe is reported as 1 × 10- 6 m, also known as

The size of a pork tapeworm is generally described in terms of

The primary goal of modern taxonomists is to

All of the following are common to both the Gram stain and the acid- fast stain EXCEPT

Which of the following classification methods relies on the morphology of organisms?

Figure 4- 1 represents a (dichotomous/taxonomic) key of the type used to identify a microbe.

A(n) (acidic/metallic/fluorescent) molecule is one that absorbs invisible radiation and emits visible light.

A primary purpose for the use of stains in microscopy is the increase the (magnification/brightness/contrast) of a specimen.

The total magnification using a 10 ocular and a 100 objective would be (110/1000/10000) X. (Be sure your answer is a numeral.)

Which of the following statements about transmission electron microscopy is FALSE?

Carbolfuchsin is the _ in the acid- fast stain.

Tungsten is a reagent used in the

A staining procedure that uses a single basic dye is called a (simple/basic/cationic) stain.

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Recombinant Dna Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Disease, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram-Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic Dna Viruses

Pathogenic Rna Viruses

Applied and Environmental Microbiology

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