Question 1

Gene silencing involves all of the following EXCEPT

Accepted Answer

A)  small interfering RNAs. 
B)  production of double stranded RNAs. 
C)  RNA-induced silencing complex. 
D)  small interfering RNA binding to a gene promoter. 
E)  Dicer. 
A)  small interfering RNAs. 
B)  production of double stranded RNAs. 
C)  RNA-induced silencing complex. 
D)  small interfering RNA binding to a gene promoter. 
E)  Dicer.

Question 2

Self-replicating DNA used to transmit a gene from one organism to another is a

Accepted Answer

A)  vector. 
B)  clone. 
C)  Southern blot. 
D)  library. 
E)  PCR. 
A)  vector. 
B)  clone. 
C)  Southern blot. 
D)  library. 
E)  PCR.

Question 3

Which of the following is an advantage of using E. coli to make a human gene product?

Accepted Answer

A)  It cannot process introns. 
B)  It does not secrete most proteins. 
C)  Endotoxin may be in the product. 
D)  Its genes are well known. 
E)  Endotoxin may be in the product and it does not secrete most proteins. 
A)  It cannot process introns. 
B)  It does not secrete most proteins. 
C)  Endotoxin may be in the product. 
D)  Its genes are well known. 
E)  Endotoxin may be in the product and it does not secrete most proteins.

Question 4

Your research group is investigating the possible use of genetically -engineered cells to produce a vaccine for malaria. List the pros and cons of using E. coli, Saccharomyces, and animal cells to produce the vaccine.

Accepted Answer

Pros and cons of using E. coli, Saccharo

Question 5

Figure 9.2
  
-In Figure 9.2, the enzyme in step 1 is

Accepted Answer

A)  DNA polymerase. 
B)  RNA polymerase. 
C)  reverse transcriptase. 
D)  DNA ligase. 
E)  spliceosome. 
A)  DNA polymerase. 
B)  RNA polymerase. 
C)  reverse transcriptase. 
D)  DNA ligase. 
E)  spliceosome.

Question 6

One of the first commercial successes of recombinant DNA technology was the production of human insulin using genetically engineered E. coli.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Accepted Answer

A)  microinjection 
B)  Ti plasmids and Agrobacterium 
C)  gene guns 
D)  electroporation 
E)  protoplast fusion 
A)  microinjection 
B)  Ti plasmids and Agrobacterium 
C)  gene guns 
D)  electroporation 
E)  protoplast fusion

Question 8

Figure 9.5
  
-In Figure 9.5, the marker genes used for selecting recombinant DNA are

Accepted Answer

A)  ampR and lacZ. 
B)  HindIII, BamHI, and EcoRI. 
C)  ampR and ori. 
D)  ori. 
E)  lacZ and ori. 
A)  ampR and lacZ. 
B)  HindIII, BamHI, and EcoRI. 
C)  ampR and ori. 
D)  ori. 
E)  lacZ and ori.

Question 9

Figure 9.2
  
-In Figure 9.2, the enzyme in step 2 is

Accepted Answer

A)  spliceosome. 
B)  reverse transcriptase. 
C)  RNA polymerase. 
D)  DNA polymerase. 
E)  DNA ligase. 
A)  spliceosome. 
B)  reverse transcriptase. 
C)  RNA polymerase. 
D)  DNA polymerase. 
E)  DNA ligase.

Question 10

Figure 9.1
  
-How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
E)  5 
A)  1 
B)  2 
C)  3 
D)  4 
E)  5

Question 11

Pieces of DNA stored in yeast cells are called a

Accepted Answer

A)  Southern blot. 
B)  clone. 
C)  vector. 
D)  library. 
E)  PCR. 
A)  Southern blot. 
B)  clone. 
C)  vector. 
D)  library. 
E)  PCR.

Question 12

Bioinformatics is the use of computer technology to compare and analyze genome sequence.

Accepted Answer

A) True 
 B)False

Question 13

Which of the following processes is NOT involved in making cDNA?

Accepted Answer

A)  reverse transcription 
B)  translation 
C)  RNA processing to remove introns 
D)  transcription 
A)  reverse transcription 
B)  translation 
C)  RNA processing to remove introns 
D)  transcription

Question 14

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is to

Accepted Answer

A)  look for a bacterium that makes the improved enzyme. 
B)  use siRNA to produce the enzyme. 
C)  mutate bacteria until one makes the improved enzyme. 
D)  determine the nucleotide sequence for the improved enzyme. 
E)  synthesize the gene for the improved enzyme. 
A)  look for a bacterium that makes the improved enzyme. 
B)  use siRNA to produce the enzyme. 
C)  mutate bacteria until one makes the improved enzyme. 
D)  determine the nucleotide sequence for the improved enzyme. 
E)  synthesize the gene for the improved enzyme.

Question 15

Figure 9.4   In Figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and X-gal will

Accepted Answer

A)  form blue, ampicillin-sensitive colonies. 
B)  form white, ampicillin-resistant colonies. 
C)  form blue, ampicillin-resistant colonies. 
D)  form white, ampicillin-sensitive colonies. 
E)  not grow. 
A)  form blue, ampicillin-sensitive colonies. 
B)  form white, ampicillin-resistant colonies. 
C)  form blue, ampicillin-resistant colonies. 
D)  form white, ampicillin-sensitive colonies. 
E)  not grow.

Question 16

The following are steps used to make DNA fingerprints. What is the third step?

Accepted Answer

A)  Lyse cells. 
B)  Add stain. 
C)  Digest with a restriction enzyme. 
D)  Perform electrophoresis. 
E)  Collect DNA. 
A)  Lyse cells. 
B)  Add stain. 
C)  Digest with a restriction enzyme. 
D)  Perform electrophoresis. 
E)  Collect DNA.

Question 17

The restriction enzyme EcoRI recognizes the sequence G↓AATTC. Which of the following is TRUE of DNA after it is treated with EcoRI?

Accepted Answer

A)  All of the DNA will have blunt ends. 
B)  All of the DNA will be circular. 
C)  All of the DNA fragments will have single-stranded regions ending in G. 
D)  All of the DNA fragments will have single-stranded regions ending in AA. 
E)  Some of the DNA will have single-stranded regions ending in AA and others will end in G. 
A)  All of the DNA will have blunt ends. 
B)  All of the DNA will be circular. 
C)  All of the DNA fragments will have single-stranded regions ending in G. 
D)  All of the DNA fragments will have single-stranded regions ending in AA. 
E)  Some of the DNA will have single-stranded regions ending in AA and others will end in G.

Question 18

A source of heat-stable DNA polymerase is

Accepted Answer

A)  Saccharomyces cerevisiae. 
B)  Pseudomonas. 
C)  Thermus aquaticus. 
D)  Bacillus thuringiensis. 
E)  Agrobacterium tumefaciens. 
A)  Saccharomyces cerevisiae. 
B)  Pseudomonas. 
C)  Thermus aquaticus. 
D)  Bacillus thuringiensis. 
E)  Agrobacterium tumefaciens.

Question 19

Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease?

Accepted Answer

A)  reverse-transcriptase PCR (rtPCR) 
B)  DNA fingerprints 
C)  restriction fragment length polymorphisms 
D)  DNA fingerprints, restriction fragment length polymorphisms, and reverse -transcriptase PCR(rtPCR) 
E)  DNA fingerprints and restriction fragment length polymorphisms 
A)  reverse-transcriptase PCR (rtPCR) 
B)  DNA fingerprints 
C)  restriction fragment length polymorphisms 
D)  DNA fingerprints, restriction fragment length polymorphisms, and reverse -transcriptase PCR(rtPCR) 
E)  DNA fingerprints and restriction fragment length polymorphisms

Question 20

Which enzyme would cut this strand of DNA? GCATGGATCCCAATGC

Accepted Answer

A)  Enzyme Recognition BamHI G↓GATCC CCCTAG↑G 
B)  Enzyme Recognition Pst ICTGC↓G G↑ACGTC 
C)  Enzyme Recognition HaeIII GG↓CC CC↑GG 
D)  Enzyme Recognition EcoRI G↓AATTC CTTAA↑G 
E)  Enzyme Recognition HindIII A↓AGCTT TTCGA↑A 
A)  Enzyme Recognition BamHI G↓GATCC CCCTAG↑G 
B)  Enzyme Recognition Pst ICTGC↓G G↑ACGTC 
C)  Enzyme Recognition HaeIII GG↓CC CC↑GG 
D)  Enzyme Recognition EcoRI G↓AATTC CTTAA↑G 
E)  Enzyme Recognition HindIII A↓AGCTT TTCGA↑A

Exam 9: Biotechnology and Dna Technology

Gene silencing involves all of the following EXCEPT

Self-replicating DNA used to transmit a gene from one organism to another is a

Which of the following is an advantage of using E. coli to make a human gene product?

Your research group is investigating the possible use of genetically -engineered cells to produce a vaccine for malaria. List the pros and cons of using E. coli, Saccharomyces, and animal cells to produce the vaccine.

Figure 9.2 -In Figure 9.2, the enzyme in step 1 is

One of the first commercial successes of recombinant DNA technology was the production of human insulin using genetically engineered E. coli.

Which of the following techniques is NOT used to introduce recombinant DNA into plants?

Figure 9.5 -In Figure 9.5, the marker genes used for selecting recombinant DNA are

Figure 9.2 -In Figure 9.2, the enzyme in step 2 is

Figure 9.1 -How many pieces will EcoRI produce from the plasmid shown in Figure 9.1?

Pieces of DNA stored in yeast cells are called a

Bioinformatics is the use of computer technology to compare and analyze genome sequence.

Which of the following processes is NOT involved in making cDNA?

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is to

Figure 9.4 In Figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and X-gal will

The following are steps used to make DNA fingerprints. What is the third step?

The restriction enzyme EcoRI recognizes the sequence G↓AATTC. Which of the following is TRUE of DNA after it is treated with EcoRI?

A source of heat-stable DNA polymerase is

Which of the following are used by the Centers for Disease Control and Prevention to track outbreaks of foodborne disease?

Which enzyme would cut this strand of DNA? GCATGGATCCCAATGC

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

The Control of Microbial Growth

Microbial Genetics

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Disease of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

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