Exam 9: Biotechnology and Dna Technology

Figure 9.1 -In Figure 9.1, after digestion with the appropriate restriction enzyme, what is the smallest piece containing the entire ampicillin-resistance (amp) gene?

A shuttle vector is a plasmid that is used to move pieces of DNA among organisms, such as bacterial, fungal, and plant cells.

Pseudomonas syringae is found naturally in the soil. Sold as Snomax, it is used to make snow at ski resorts. The same bacterium with a gene deletion (Ice-minus) is used to prevent ice formation on plants. Should Snomax and Ice-minus be considered modified organisms and subject to precautions of releasing genetically modified organisms? Explain why or why not.

Assume you have discovered a cell that produces a lipase that works in cold water for a laundry additive. You can increase the efficiency of this enzyme by changing one amino acid. This is done by

In recombinant DNA technology, a vector is a self-replicating segment of DNA, such as a plasmid or viral genome.

You want to determine whether a person has a certain mutant gene. The process involves using a primer and a heat-stable DNA polymerase. This process is

Which of the following is NOT an agricultural product made by DNA techniques?

The term biotechnology refers exclusively to the use of genetically engineered organisms for the production of desired products.

The development of DNA technology is bringing profound changes to science, agriculture and healthcare. Provide one example of a DNA technology and provide at least one advantage and one example of a concern or problem associated with its use.

The Human Genome Project, which was completed in 2003, was focused on

Which of the following are used to silence specific genes and hold promise for treating cancer or viral diseases, such as hepatitis B?

Which of the following methods would be used to introduce the plasmid shown in Figure 9.5 into E. coli?

The Ti plasmid isolated from Agrobacterium can be used to insert DNA into any type of plant.

An advantage of synthetic DNA over genomic or cDNA is the ability to

The reaction catalyzed by reverse transcriptase is

The practice of breeding plants and animals for desirable traits, such as high crop yield, is called natural selection.

You have a small gene that you wish replicated by PCR. After 3 replication cycles, how many double-stranded DNA molecules do you have?

In the Southern blot technique, which of the following is NOT required?

Figure 9.3 The figure at the left in Figure 9.3 shows a gene identified by Southern blotting. What will a Southern blot of the same gene look like after PCR?