Question 1

Which one of the following is most useful for disinfecting medical instruments?

Accepted Answer

A)  Quats 
B)  Benzoic acid 
C)  Phenol 
D)  Bisphenols 
E)  Chlorine 
A)  Quats 
B)  Benzoic acid 
C)  Phenol 
D)  Bisphenols 
E)  Chlorine

Question 2

Figure 7.3
Assume 109 E. coli cells/ml are in a flask.
  
-Which graph in Figure 7.3 best depicts the effect of placing the culture in an autoclave for 15 min. at time x?

Accepted Answer

A)  a 
B)  b 
C)  c 
D)  d 
E)  e 
A)  a 
B)  b 
C)  c 
D)  d 
E)  e

Question 3

Which of the following does not achieve sterilization?

Accepted Answer

A)  Ethylene oxide 
B)  Autoclave 
C)  Dry heat 
D)  Formaldehyde 
E)  Pasteurization 
A)  Ethylene oxide 
B)  Autoclave 
C)  Dry heat 
D)  Formaldehyde 
E)  Pasteurization

Question 4

Which of the following pairs is not correctly matched?

Accepted Answer

A)  Merthiolate - open wounds 
B)  Ag - burns 
C)  CuSO₄ - algicide 
D)  H₂O₂ - open wounds 
E)  None of the above 
A)  Merthiolate - open wounds 
B)  Ag - burns 
C)  CuSO₄ - algicide 
D)  H₂O₂ - open wounds 
E)  None of the above

Question 5

Which of the following is not effective against nonenveloped viruses?

Accepted Answer

A)  Ozone 
B)  Alcohol 
C)  Chlorine 
D)  Ethylene oxide 
E)  All are equally effective 
A)  Ozone 
B)  Alcohol 
C)  Chlorine 
D)  Ethylene oxide 
E)  All are equally effective

Question 6

Foods are preserved with all of the following except

Accepted Answer

A)  Potassium sorbate. 
B)  Sodium propionate. 
C)  Sodium nitrite. 
D)  Biguanides. 
E)  Nisin. 
A)  Potassium sorbate. 
B)  Sodium propionate. 
C)  Sodium nitrite. 
D)  Biguanides. 
E)  Nisin.

Question 7

Figure 7.1
  
-In Figure 7.1, what is the thermal death time for culture a?

Accepted Answer

A)  150°C 
B)  60 min. 
C)  100°C 
D)  120 min. 
E)  Can't tell 
A)  150°C 
B)  60 min. 
C)  100°C 
D)  120 min. 
E)  Can't tell

Question 8

Which of the following is best to sterilize heat- labile solutions?

Accepted Answer

A)  Pasteurization 
B)  Dry heat 
C)  Autoclave 
D)  Membrane filtration 
E)  None of the above 
A)  Pasteurization 
B)  Dry heat 
C)  Autoclave 
D)  Membrane filtration 
E)  None of the above

Question 9

Which of these is not an oxidizing agent?

Accepted Answer

A)  Ozone 
B)  Iodine 
C)  Hydrogen peroxide 
D)  Chlorine 
E)  None of the above 
A)  Ozone 
B)  Iodine 
C)  Hydrogen peroxide 
D)  Chlorine 
E)  None of the above

Question 10

Glutaraldehyde is considered one of the most effective disinfectants for hospital use. Which of the following would not be true about glutaraldehyde?

Accepted Answer

A)  Safe to transport. 
B)  Stains and corrodes. 
C)  Attacks all microorganisms. 
D)  Acts rapidly. 
E)  Not hampered by organic material. 
A)  Safe to transport. 
B)  Stains and corrodes. 
C)  Attacks all microorganisms. 
D)  Acts rapidly. 
E)  Not hampered by organic material.

Question 11

Which of the following could be used to sterilize plastic Petri plates in a plastic wrapper?

Accepted Answer

A)  Ultraviolet radiation 
B)  Sunlight 
C)  Microwaves 
D)  Gamma radiation 
E)  None of the above 
A)  Ultraviolet radiation 
B)  Sunlight 
C)  Microwaves 
D)  Gamma radiation 
E)  None of the above

Question 12

Which of the following substances can sterilize?

Accepted Answer

A)  Chlorine 
B)  Phenolics 
C)  Ethylene oxide 
D)  Alcohol 
E)  Soap 
A)  Chlorine 
B)  Phenolics 
C)  Ethylene oxide 
D)  Alcohol 
E)  Soap

Question 13

Which of the following substances is the least effective antimicrobial agent?

Accepted Answer

A)  Cationic detergents 
B)  Phenolics 
C)  Iodine 
D)  Soap 
E)  Alcohol 
A)  Cationic detergents 
B)  Phenolics 
C)  Iodine 
D)  Soap 
E)  Alcohol

Question 14

Bacterial death will result from damage to which of the following structures?

Accepted Answer

A)  Proteins 
B)  Cell wall 
C)  Nucleic acids 
D)  Plasma membrane 
E)  All of the above 
A)  Proteins 
B)  Cell wall 
C)  Nucleic acids 
D)  Plasma membrane 
E)  All of the above

Question 15

Which of the following best describes the pattern of microbial death?

Accepted Answer

A)  The pattern varies depending on the species. 
B)  The pattern varies depending on the antimicrobial agent. 
C)  The cells in a population die at a constant rate. 
D)  All of the cells in a culture are never killed. 
E)  All the cells in a culture die at once. 
A)  The pattern varies depending on the species. 
B)  The pattern varies depending on the antimicrobial agent. 
C)  The cells in a population die at a constant rate. 
D)  All of the cells in a culture are never killed. 
E)  All the cells in a culture die at once.

Question 16

Table 7.3
The following data were obtained by incubating the bacteria in nutrient medium + disinfectant for 24 hr, then transferring one loopful to nutrient medium (subculturing.) $\quad\quad\quad\quad\quad\quad\quad\quad \underline{\text { Doom
} } \quad \quad\quad\quad\quad\quad\quad\underline{K O}$
$\begin{array}{rrrrr}
\text { Dilution } & \text { Initial } & \text { Subculture } & \text { Initial } & \text { Subculture } \
1: 16 & - & + & + & + \
1: 32 & - & + & + & + \
1: 64 & - & + & + & + \
1: 128 & + & + & + & +
\end{array}$

$\quad\quad\quad\quad\quad\quad\quad\quad \underline{\text { Mortum }} \quad\quad\quad\quad\quad\quad  \underline{\text { Sterl }}$
$\begin{array}{rrrrrr}\text { Dilution } & \text { Initial } & \text { Subculture } & \text { Initial } & \text { Subculture } \
1: 16 & - & - & - & + \
1: 32 & - & + & + & + \
1: 64 & + & + & + & + \
1: 128 & + & + & + & +
\end{array}$
-In Table 7.3, which disinfectant was bactericidal?

Accepted Answer

A)  Doom 
B)  K.O. 
C)  Mortum 
D)  Sterl 
E)  Can't tell 
A)  Doom 
B)  K.O. 
C)  Mortum 
D)  Sterl 
E)  Can't tell

Question 17

Which of the following treatments is the most effective for controlling microbial growth?

Accepted Answer

A)  140°C for < 1 sec. 
B)  63°C for 30 min. 
C)  72°C for 15 sec. 
D)  They are equivalent treatments. 
E)  None are effective. 
A)  140°C for < 1 sec. 
B)  63°C for 30 min. 
C)  72°C for 15 sec. 
D)  They are equivalent treatments. 
E)  None are effective.

Question 18

Table 7.1
A disk- diffusion test using Staphylococcus gave the following results: $\begin{array}{cc} \underline{\text { Disinfectant }}& \underline{\text { Zone of inhibition (mm) }}\
\text { A } & 0 \
\text { B } & 2.5 \
\text { C } & 10 \
\text { D } & 5
\end{array}$
-In Table 7.1, which compound was the most effective against E. coli?

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  D 
E)  Can't tell 
A)  A 
B)  B 
C)  C 
D)  D 
E)  Can't tell

Question 19

Iodophors differ from iodine (I2) in that iodophors

Accepted Answer

A)  Are combined with a nonionic detergent. 
B)  Are longer lasting. 
C)  Don't stain. 
D)  Are less irritating. 
E)  All of the above. 
A)  Are combined with a nonionic detergent. 
B)  Are longer lasting. 
C)  Don't stain. 
D)  Are less irritating. 
E)  All of the above.

Question 20

Which of the following is not a direct result of heat?

Accepted Answer

A)  Denaturing enzymes 
B)  Breaking hydrogen bonds 
C)  Breaking sulfhydryl bonds 
D)  Cell lysis 
E)  None of the above 
A)  Denaturing enzymes 
B)  Breaking hydrogen bonds 
C)  Breaking sulfhydryl bonds 
D)  Cell lysis 
E)  None of the above

Exam 7: The Control of Microbial Growth

Which one of the following is most useful for disinfecting medical instruments?

Figure 7.3 Assume 109 E. coli cells/ml are in a flask. -Which graph in Figure 7.3 best depicts the effect of placing the culture in an autoclave for 15 min. at time x?

Which of the following does not achieve sterilization?

Which of the following pairs is not correctly matched?

Which of the following is not effective against nonenveloped viruses?

Foods are preserved with all of the following except

Figure 7.1 -In Figure 7.1, what is the thermal death time for culture a?

Which of the following is best to sterilize heat- labile solutions?

Which of these is not an oxidizing agent?

Glutaraldehyde is considered one of the most effective disinfectants for hospital use. Which of the following would not be true about glutaraldehyde?

Which of the following could be used to sterilize plastic Petri plates in a plastic wrapper?

Which of the following substances can sterilize?

Which of the following substances is the least effective antimicrobial agent?

Bacterial death will result from damage to which of the following structures?

Which of the following best describes the pattern of microbial death?

Which of the following treatments is the most effective for controlling microbial growth?

Table 7.1 A disk- diffusion test using Staphylococcus gave the following results: A 0 B 2.5 C 10 D 5 -In Table 7.1, which compound was the most effective against E. coli?

Iodophors differ from iodine (I2) in that iodophors

Which of the following is not a direct result of heat?

The Microbial World and You

Chemical Principles

Observing Microorganisms Through a Microscope

Functional Anatomy of Prokaryotic and Eukaryotic Cells

Microbial Metabolism

Microbial Growth

Microbial Genetics

Biotechnology and Recombinant DNA

Classification of Microorganisms

The Prokaryotes: Domains Bacteria and Archaea

The Eukaryotes: Fungi, Algae, Protozoa, and Helminths

Viruses, Viroids, and Prions

Principles of Disease and Epidemiology

Microbial Mechanisms of Pathogenicity

Innate Immunity: Nonspecific Defenses of the Host

Adaptive Immunity: Specific Defenses of the Host

Practical Applications of Immunology

Disorders Associated With the Immune System

Antimicrobial Drugs

Microbial Diseases of the Skin and Eyes

Microbial Diseases of the Nervous System

Microbial Diseases of the Cardiovascular and Lymphatic Systems

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Environmental Microbiology

Applied and Industrial Microbiology

Filters