Question 1

In order for the yeast galactose metabolizing gene, GAL1, to be transcribed, the regulatory protein produced by the GAL4 gene binds to

Accepted Answer

A)  the operator sequence. 
B)  the repressor protein. 
C)  upstream activator sequence G (UASG) region. 
D)  RNA polymerase. 
E)  a ribosome. 
A)  the operator sequence. 
B)  the repressor protein. 
C)  upstream activator sequence G (UASG) region. 
D)  RNA polymerase. 
E)  a ribosome. C

Question 2

A DNA polymorphism is defined as two or more alternative forms or alleles differing in sequence at a specific chromosomal locus.

Accepted Answer

A) True 
 B)False  True

Question 3

Briefly explain how Ti plasmids induce crown gall (tumor) growth in plants parasitized by A. tumefaciens, highlighting the specific properties that make this plasmid so useful in biotechnology.

Accepted Answer

Ti plasmids are so-named because they carry tumor-inducing genes. The plasmid enters (transforms) a host cell, and a 30-kb region called transforming- or T-DNA integrates by recombination with the host DNA. The T-DNA region carries genes for plant cell transformation, creating the crown gall where Agrobacterium flourishes under natural conditions. The T-DNA region necessary for successful excision, transfer, and integration consists of only its 25-bp terminal repeats, which means the rest of the DNA in this region can be replaced with DNA of interest, and the whole plasmid engineered to vector this DNA.

Question 4

A couple with STR genotypes (50, 75) and (50, 100) (where the allele numbers denote repeat length) have several children. What are the possible genotypes of their children for this STR locus?

Accepted Answer

As for any single-locus trait,

Question 5

To study how protein interactions affect cellular function, ________ is the most appropriate method.

Accepted Answer

A)  RT-PCR 
B)  Southern blotting 
C)  the two-hybrid system 
D)  PCR 
E)  restriction mapping 
A)  RT-PCR 
B)  Southern blotting 
C)  the two-hybrid system 
D)  PCR 
E)  restriction mapping

Question 6

Prior to the successful and cloning and industrial-scale manufacture, substances like blood clotting factor VIII (used to treat hemophilia) had to be collected from people. What dangers inherent in this are avoided by engineering a recombinant molecule?

Accepted Answer

Despite exacting standards and efforts a

Question 7

Ti plasmids and the T-DNA system can be used to transform any vascular plant.

Accepted Answer

A) True 
 B)False

Question 8

Monsanto Corporation developed transgenic crop plants conferring tolerance of the herbicide RoundupTM (glyphosate), which Monsanto owns. What are some pros and cons, in ecological and economic terms, of producing such herbicide-resistant plants?

Accepted Answer

One advantage to farmers is the opportun

Question 9

Somatic cell gene therapy can result in a cure for a genetic disease in an individual as well as any progeny produced by the individual.

Accepted Answer

A) True 
 B)False

Question 10

In yeast, expression of galactose utilization genes is induced when there is ________ in the culture medium.

Accepted Answer

A)  lactose 
B)  glucose 
C)  maltose 
D)  galactose 
E)  Any of the above 
A)  lactose 
B)  glucose 
C)  maltose 
D)  galactose 
E)  Any of the above

Question 11

Chromosome walking involves

Accepted Answer

A)  cloning a gene in stepwise fashion using partially overlapping subclones. 
B)  use of PCR to generate fragments of chromosomes. 
C)  constructing a cDNA library. 
D)  G-Staining and labeling the banding series of each chromosome. 
E)  none of the above. 
A)  cloning a gene in stepwise fashion using partially overlapping subclones. 
B)  use of PCR to generate fragments of chromosomes. 
C)  constructing a cDNA library. 
D)  G-Staining and labeling the banding series of each chromosome. 
E)  none of the above.

Question 12

Allelic variants differing by a single nucleotide are called

Accepted Answer

A)  monoallelic polymorphisms (MAPs). 
B)  monomorphic alleles (MMAs). 
C)  uninucleotidic variants (UNVs). 
D)  single-nucleotide polymorphisms (SNPs). 
E)  none of the above. 
A)  monoallelic polymorphisms (MAPs). 
B)  monomorphic alleles (MMAs). 
C)  uninucleotidic variants (UNVs). 
D)  single-nucleotide polymorphisms (SNPs). 
E)  none of the above.

Question 13

When yeast cells are cultured in the presence of both glucose and galactose, the GAL genes are

Accepted Answer

A)  transcribed and translated. 
B)  neither transcribed nor translated. 
C)  transcribed but not translated. 
D)  translated but not transcribed. 
E)  none of the above. 
A)  transcribed and translated. 
B)  neither transcribed nor translated. 
C)  transcribed but not translated. 
D)  translated but not transcribed. 
E)  none of the above.

Question 14

The Human Genome Project was launched in ________, and the complete working draft was announced in ________.

Accepted Answer

A)  1997, 2001 
B)  1953, 1985 
C)  1990, 2001 
D)  1953, 2001 
E)  1990, 1997 
A)  1997, 2001 
B)  1953, 1985 
C)  1990, 2001 
D)  1953, 2001 
E)  1990, 1997

Question 15

Much plant genetic engineering is accomplished with the soil bacterium Agrobacter tumefaciens.

Accepted Answer

A) True 
 B)False

Question 16

Antisense mRNA can be used to regulate the expression of genes. Briefly describe how this technology was used to slow the ripening of Calgene's ill-fated Flavr-SavrTM tomato.

Accepted Answer

In engineered tomatoes, the gene for pol

Question 17

Briefly describe some of the applications of DNA fingerprinting in (1) criminal justice, (2) enforcing game laws, (3) studying ancient organisms, and (4) conservation genetics.

Accepted Answer

In criminal justice, DNA fingerprinting

Question 18

Prenatal genetic tests cannot determine

Accepted Answer

A)  the gender of a fetus. 
B)  if a fetus has a specific mutation. 
C)  the DNA fingerprint for certain VNTR loci in a fetus. 
D)  if chromosomal abnormalities exist in a fetus. 
E)  All of these can be determined. 
A)  the gender of a fetus. 
B)  if a fetus has a specific mutation. 
C)  the DNA fingerprint for certain VNTR loci in a fetus. 
D)  if chromosomal abnormalities exist in a fetus. 
E)  All of these can be determined.

Question 19

Variable-number tandem repeats can differ in

Accepted Answer

A)  length of repeat motif. 
B)  number of units repeating in tandem. 
C)  base sequence in repeat motif. 
D)  A and C 
E)  A, B, and C 
A)  length of repeat motif. 
B)  number of units repeating in tandem. 
C)  base sequence in repeat motif. 
D)  A and C 
E)  A, B, and C

Question 20

DNA fingerprinting can be used to convict criminals but has little application in exonerating individuals already convicted.

Accepted Answer

A) True 
 B)False

Exam 17: Applications of Recombinant DNA Technology

In order for the yeast galactose metabolizing gene, GAL1, to be transcribed, the regulatory protein produced by the GAL4 gene binds to

A DNA polymorphism is defined as two or more alternative forms or alleles differing in sequence at a specific chromosomal locus.

Briefly explain how Ti plasmids induce crown gall (tumor) growth in plants parasitized by A. tumefaciens, highlighting the specific properties that make this plasmid so useful in biotechnology.

A couple with STR genotypes (50, 75) and (50, 100) (where the allele numbers denote repeat length) have several children. What are the possible genotypes of their children for this STR locus?

To study how protein interactions affect cellular function, ________ is the most appropriate method.

Prior to the successful and cloning and industrial-scale manufacture, substances like blood clotting factor VIII (used to treat hemophilia) had to be collected from people. What dangers inherent in this are avoided by engineering a recombinant molecule?

Ti plasmids and the T-DNA system can be used to transform any vascular plant.

Monsanto Corporation developed transgenic crop plants conferring tolerance of the herbicide RoundupTM (glyphosate), which Monsanto owns. What are some pros and cons, in ecological and economic terms, of producing such herbicide-resistant plants?

Somatic cell gene therapy can result in a cure for a genetic disease in an individual as well as any progeny produced by the individual.

In yeast, expression of galactose utilization genes is induced when there is ________ in the culture medium.

Chromosome walking involves

Allelic variants differing by a single nucleotide are called

When yeast cells are cultured in the presence of both glucose and galactose, the GAL genes are

The Human Genome Project was launched in ________, and the complete working draft was announced in ________.

Much plant genetic engineering is accomplished with the soil bacterium Agrobacter tumefaciens.

Antisense mRNA can be used to regulate the expression of genes. Briefly describe how this technology was used to slow the ripening of Calgene's ill-fated Flavr-SavrTM tomato.

Briefly describe some of the applications of DNA fingerprinting in (1) criminal justice, (2) enforcing game laws, (3) studying ancient organisms, and (4) conservation genetics.

Prenatal genetic tests cannot determine

Variable-number tandem repeats can differ in

DNA fingerprinting can be used to convict criminals but has little application in exonerating individuals already convicted.

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Advanced Gene Mapping in Eukaryotes

Gene Mapping in Eukaryotes

Quantitative Genetics

Extensions of Mendelian Genetic Principles

Chromosomal Basis of Inheritance

Molecular Evolution

Population Genetics

Non-Mendelian Inheritance

Genetics of Cancer

Genetic Analysis of Development

Regulation of Gene Expression in Eukaryotes

Mendelian Genetics

Regulation of Gene Expression in Bacteria and Bacteriophages

Genomics

Recombinant DNA Technology

DNA Mutation, DNA Repair, and Transposable Elements

Gene Expression: Translation

Gene Expression: Transcription

Gene Control of Proteins

DNA Replication

DNA: The Genetic Material

Genetics: an Introduction

Filters

The Human Genome Project was launched in , and the complete working draft was announced in .