Question 1

The first complete genome sequenced was that of

Accepted Answer

A)  Mus musculus. 
B)  Methanococcus. 
C)  the human mitochondrion. 
D)  Saccharomyces cerevisiae. 
E)  Escherichia coli. 
A)  Mus musculus. 
B)  Methanococcus. 
C)  the human mitochondrion. 
D)  Saccharomyces cerevisiae. 
E)  Escherichia coli. C

Question 2

Describe a DNA microarray setup for assaying mutations associated with a hypothetical genetic disease.

Accepted Answer

Normal and mutant gene sequences associated with the disease could be affixed to the array in known positions. DNA from a patient and a known nonmutant individual could be separately isolated and labeled (or mRNA could be used, making cDNA for labeling with reverse transcription). Both are applied to the microarray, and whichever labeled fragments binds with the DNA fragments on the array will reveal colorimetrically which alleles the patient possesses.

Question 3

Which of the following restriction enzymes is most useful as a tool in genome sequencing?

Accepted Answer

A)  HindIII 
B)  BamHI 
C)  HpaII 
D)  EcoRI 
E)  NotI 
A)  HindIII 
B)  BamHI 
C)  HpaII 
D)  EcoRI 
E)  NotI E

Question 4

The distribution of coding genes is surprisingly even throughout the human genome.

Accepted Answer

A) True 
 B)False

Question 5

Why is the proteome, the number of proteins produced by cells, so much greater than the number of genes in eukaryotes?

Accepted Answer

Eukaryotic translation is characterized

Question 6

Sequencing the dog genome is a high priority. What insights do you think can be gained about the evolutionary process by analyzing the genome of dogs?

Accepted Answer

Dogs are diversified into a multitude of

Question 7

The human genome consists mostly of noncoding DNA.

Accepted Answer

A) True 
 B)False

Question 8

If bacterial sequences in the human genome represent horizontal transfer, the transfer may have occurred in early humans, or in the more distant past, in primate ancestors of humans. In the latter scenario, modern humans inherited such sequences from a common ancestor. Can you think of a way to test the hypothesis that bacterial sequences were vectored to humans vs. vectored to an ancestral primate?

Accepted Answer

Check for the presence of identical sequ

Question 9

Discuss the relative roles of descriptive and hypothesis-driven science in genomics.

Accepted Answer

This chapter points out that merely sequ

Question 10

Archaea are typically found in much the same habitats as eubacteria.

Accepted Answer

A) True 
 B)False

Question 11

One of the great surprises of the human genome was the number of genes and other sequences of prokaryotic origin (about 220). Speculate on how these bacterial genes may have become integrated into the human genome.

Accepted Answer

These sequences are probably e

Question 12

________ were the first class of genetic marker developed by geneticists.

Accepted Answer

A)  microsatellites 
B)  RFLPs 
C)  SNPs 
D)  STRs 
E)  VNTRs 
A)  microsatellites 
B)  RFLPs 
C)  SNPs 
D)  STRs 
E)  VNTRs

Question 13

A SNP represents a change in

Accepted Answer

A)  a three-nucleotide sequence. 
B)  the number of restriction sites. 
C)  the number of nucleotides between restriction sites. 
D)  the number of tandem repeats. 
E)  none of the above. 
A)  a three-nucleotide sequence. 
B)  the number of restriction sites. 
C)  the number of nucleotides between restriction sites. 
D)  the number of tandem repeats. 
E)  none of the above.

Question 14

RFLPs arise by

Accepted Answer

A)  changes in the number of tandem repeats. 
B)  base changes that add or delete restriction enzyme recognition sites. 
C)  addition or deletion of bases between restriction enzyme recognition sites. 
D)  A, B, and C 
E)  B and C only 
A)  changes in the number of tandem repeats. 
B)  base changes that add or delete restriction enzyme recognition sites. 
C)  addition or deletion of bases between restriction enzyme recognition sites. 
D)  A, B, and C 
E)  B and C only

Question 15

The "transcriptome" is the complete set of polypeptides produced by an organism.

Accepted Answer

A) True 
 B)False

Question 16

The ________ method was used by the Celera Genomics corporation to sequence the human genome.

Accepted Answer

A)  clone contig 
B)  radiation hybrid 
C)  pedigree analysis 
D)  FISH 
E)  direct shotgun 
A)  clone contig 
B)  radiation hybrid 
C)  pedigree analysis 
D)  FISH 
E)  direct shotgun

Question 17

The subfield of genomics that deals with gene expression and interaction is

Accepted Answer

A)  hypothetical genomics. 
B)  functional genomics. 
C)  expression genomics. 
D)  structural genomics. 
E)  comparative genomics. 
A)  hypothetical genomics. 
B)  functional genomics. 
C)  expression genomics. 
D)  structural genomics. 
E)  comparative genomics.

Question 18

Humans have 2N = 46 chromosomes, so the human genome consists of the sequence data of 23 chromosomes.

Accepted Answer

A) True 
 B)False

Question 19

A genetic map shows

Accepted Answer

A)  the relative distance between genes on chromosomes. 
B)  the location of genes on chromosomes. 
C)  the genetic component of key phenotypic traits. 
D)  A and B 
E)  A, B, and C 
A)  the relative distance between genes on chromosomes. 
B)  the location of genes on chromosomes. 
C)  the genetic component of key phenotypic traits. 
D)  A and B 
E)  A, B, and C

Question 20

Contrast mapping and shotgun sequencing approaches; what are the strengths and weaknesses of each approach?

Accepted Answer

The mapping approach involves generating

Exam 18: Recombinant DNA Technology

The first complete genome sequenced was that of

Describe a DNA microarray setup for assaying mutations associated with a hypothetical genetic disease.

Which of the following restriction enzymes is most useful as a tool in genome sequencing?

The distribution of coding genes is surprisingly even throughout the human genome.

Why is the proteome, the number of proteins produced by cells, so much greater than the number of genes in eukaryotes?

Sequencing the dog genome is a high priority. What insights do you think can be gained about the evolutionary process by analyzing the genome of dogs?

The human genome consists mostly of noncoding DNA.

Discuss the relative roles of descriptive and hypothesis-driven science in genomics.

Archaea are typically found in much the same habitats as eubacteria.

One of the great surprises of the human genome was the number of genes and other sequences of prokaryotic origin (about 220). Speculate on how these bacterial genes may have become integrated into the human genome.

________ were the first class of genetic marker developed by geneticists.

A SNP represents a change in

RFLPs arise by

The "transcriptome" is the complete set of polypeptides produced by an organism.

The ________ method was used by the Celera Genomics corporation to sequence the human genome.

The subfield of genomics that deals with gene expression and interaction is

Humans have 2N = 46 chromosomes, so the human genome consists of the sequence data of 23 chromosomes.

A genetic map shows

Contrast mapping and shotgun sequencing approaches; what are the strengths and weaknesses of each approach?

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Advanced Gene Mapping in Eukaryotes

Gene Mapping in Eukaryotes

Quantitative Genetics

Extensions of Mendelian Genetic Principles

Chromosomal Basis of Inheritance

Molecular Evolution

Population Genetics

Non-Mendelian Inheritance

Genetics of Cancer

Genetic Analysis of Development

Regulation of Gene Expression in Eukaryotes

Mendelian Genetics

Regulation of Gene Expression in Bacteria and Bacteriophages

Genomics

Applications of Recombinant DNA Technology

DNA Mutation, DNA Repair, and Transposable Elements

Gene Expression: Translation

Gene Expression: Transcription

Gene Control of Proteins

DNA Replication

DNA: The Genetic Material

Genetics: an Introduction

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