Exam 2: Observing the Microbial Cell

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What happens to an electron when excited by light at the excitation wavelength?

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List and briefly describe four ways that light interacts with objects.

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Which of the following would be MOST appropriate to visualize viral particles being assembled inside an infected bacterial cell?

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Chlamydia trachomatis transmits infection to a new cell via

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What is the best explanation for a Gram-negative bacterium appearing purple after performing a Gram stain?

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Which type of microscopy is particularly useful to study the surfaces of live bacteria?

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The part of the human eye that is MOST involved in resolving an image is the

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Which two components of the Gram stain form a complex that is retained by Gram-positive cells?

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Which of these techniques would provide the best resolution of an enzyme's structure?

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What would happen if a lens had the same refractive index as air?

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A(n) ________ acts to vary the diameter of the light column in a light microscope.

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Describe three methods of sample preparation for electron microscopy. Which method would cause the fewest artifacts? Why?

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What color are Gram-positive and Gram-negative cells when properly Gram stained? For each step of the Gram-stain procedure, predict the colors of a Gram-positive or Gram-negative cell if that step were omitted during staining. Explain your reasoning.

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Which of the following methods is used to generate the structure shown below? Which of the following methods is used to generate the structure shown below?

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Resolution is the smallest distance by which two objects can be ________ and still be ________.

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Which type of microscopy gives a "false 3-D image"?

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Why are stains used in microscopy? Compare and contrast the stains used in light versus electron microscopy.

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Transmission electron microscopy commonly has a resolution of ________ the highest resolution possible for light microscopy.

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List three different differential stains used in microbiology. What can be detected with each?

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Label the condenser in the figure below. Label the condenser in the figure below.

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