Exam 5: Protein Purification and Characterization Techniques

The purpose of using nitrocellulose for a western blot is to

The order of elution of AAs H, E, & K from a cation exchange column by a pH 6 buffer is

A separation of a mixture of cations of different charge requires

The degree of separation in molecular sieve chromatography depends on

Which of the following happens as a protein is purified?

Which separates based on the ionic charge on a protein?

Which type of column is most affected by the shape of the protein, for example, comparing spherical and cigar-shaped proteins?

The most common way of visualizing the results seen on a protein chip is by use of

Determination of the sequence of amino acids in a peptide is done by

Which of the following is not an example of column chromatography?

Matrix-Assisted Laser Desorption Ionization is a type of _____ technique.

In electrophoresis experiments

In a sample consisting of lysine, leucine, and glutamic acid, which will be eluted last from an anion exchange resin at pH 7?

A protein chip is functionally most similar to which other technique?

Identify a proteolytic enzyme that preferentially hydrolyzes amide bonds adjacent to aromatic amino acid residues.

Which of the following polysaccharides is used to make up resins for use in electrophoresis and in column chromatography?

The most efficient method for determining the sequence of a short peptide is:

Ammonium sulfate is useful in protein purification because

Salting out with ammonium sulfate is based upon proteins interacting with other proteins via

Two-dimensional electrophoresis usually exploits these 2 different properties of proteins: