Exam 13: Nucleic Acid Biotechnology Techniques

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Refer to Exhibit 13A. If the region between the 2 sites for Hgi EII were deleted, the resulting plasmid

When fluorescent markers are used for DNA sequencing:

A multiple cloning site is also known as:

Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β -gal gene codes for the enzyme β -galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the Amp^R gene confers ampicillin resistance. Refer to Exhibit 13D. Neglecting any discussion of whether it's a good or bad choice, I attempt to insert a gene fragment into the Hind III site and transform bacteria with the plasmid. How can I tell which transformants have the insert?

Modern genetic engineering allows the following manipulation, which was not available using traditional breeding methods:

A palindrome is

The same gene on two different paired chromosomes in eukaryotes are called

One major obstacle to using bacteria to produce human proteins is bacteria do not have the cellular apparatus to splice introns out of mRNA.

When fluorescence detection methods are used in biotechnology they

The application of gel electrophoresis (PAGE) in DNA sequencing

The use of the Bt gene is prohibited in corn plants.

Determining disease states using protein arrays may be limited by

Epogen is

In recombinant DNA technology

Recombinant DNA molecules contain covalently linked segments derived from two or more sources.

Which of the following statements about plants which contain the recombinant form of the gene from Bacillus thuringiensis (Bt gene), is true ?

Enzymes that seal nicks in DNA are called

Microarrays allow the analysis of thousands of samples of DNA or protein to be performed simultaneously.

Genetic engineering

Which of the following parameters affects the distance DNA molecules migrate during electrophoresis, at pH = 8?