Exam 10: Biotechnology

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Match the steps of two rounds of PCR with appropriate description.
Match the steps of two rounds of PCR with appropriate description.
Premises:
The mixture is heated and the double-stranded DNA separates into single strands. When the mixture is cooled, some of the primers base-pair with the DNA at opposite ends of the targeted sequence.
Responses:
E
C
D
Correct Answer:
Verified
Premises:
Responses:
The mixture is heated and the double-stranded DNA separates into single strands. When the mixture is cooled, some of the primers base-pair with the DNA at opposite ends of the targeted sequence.
E
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Correct Answer:
Verified

Match the following organisms to the description with which they best correspond.
Premises:
Researchers studied these transgenic animals resulting in much of our current understanding of how diabetes works in humans.
Responses:
A. tumefaciens
goats
mice
Correct Answer:
Verified
Premises:
Responses:
Researchers studied these transgenic animals resulting in much of our current understanding of how diabetes works in humans.
A. tumefaciens
Free
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Correct Answer:
Verified

The term "biotech barnyards" refers to ____.

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Correct Answer:
Verified

A

In a PCR reaction, a few seconds at high temperature disrupts the ____ that hold the two strands of DNA together, so every molecule of DNA unwinds and becomes single-stranded.

(Multiple Choice)
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Many restriction enzymes generate ____ on DNA fragments after being cut.

(Multiple Choice)
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The APOE gene encodes ____.

(Multiple Choice)
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A DNA molecule that can accept foreign DNA and be replicated inside a host cell is known as a ____.

(Multiple Choice)
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Match the following terms to the definition with which they best correspond.
Premises:
An organism's complete set of genetic material.
Responses:
genome
probe
DNA ligase
Correct Answer:
Verified
Premises:
Responses:
An organism's complete set of genetic material.
genome
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If the nucleotide sequence ACTGAG represents the sticky end of a DNA molecule, to what other sticky end sequence would it base pair?

(Multiple Choice)
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An example of a restriction enzyme is ____.

(Multiple Choice)
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The following items are related to DNA recombinant technology. Match the terms to the statements with which they correspond.
Premises:
DNA fragment that contains the DNA of more than one species
Responses:
DNA polymerase
clones
restriction enzymes
Correct Answer:
Verified
Premises:
Responses:
DNA fragment that contains the DNA of more than one species
DNA polymerase
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After DNA fragments with complementary sticky ends base pair, ____seals the gaps between the fragments to produce a continuous DNA molecule.

(Multiple Choice)
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The E4 variation of the APOE gene increases a person's chances of developing ____.

(Multiple Choice)
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Crops have been genetically modified to resist the herbicide ____.

(Multiple Choice)
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In agriculture, the use of GMOs is regulated by the ____.

(Multiple Choice)
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The entire set of genetic material of a given individual is its ____. The entire set of genetic material of a given individual is its ____.

(Multiple Choice)
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The restriction enzyme Eco RI recognizes the sequence GAATTC; therefore, it will ____ whenever it encounters this sequence.

(Multiple Choice)
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The discovery of ____ allowed researchers to cut chromosomes into manageable-sized DNA fragments.

(Multiple Choice)
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After obtaining DNA fragments by PCR, the fragments are ____ to reveal the lengths of the short tandem repeats.

(Multiple Choice)
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Approximately ____% of a person's 3 billion nucleotides are unique to that individual.

(Multiple Choice)
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