Exam 2: Nucleic Acids

EcoRI recognizes the sequence 5'-G $\downarrow$ AATTC-3' (the arrow indicates the point of cleavage). Treatment of the following oligonucleotide with EcoRI would produce two oligonucleotides with sizes of _____ nucleotides containing _____ ends. 5'-AAGTCGATACAGAATTCGTACCTAG-3'

Which of the following represents the correct order of steps in a PCR reaction beginning with double-stranded DNA?

In bacteria, riboflavin can inhibit its own synthesis by binding to an mRNA element termed a ______.

Because of the high temperature _____ step during a PCR reaction, the DNA polymerase from _____ is used.

Prokaryotes contain nucleotide sequences known as _____ that contain related genes with related functions.

Which strand of DNA is replicated exclusively in a discontinuous fashion?

Transfer RNA molecules are involved in ______.

What technique involves addition of primers that do not exactly match the sequence of a gene, thus allowing for introduction of a mutation?

This is the structure of cytosine. a. What are the names of the other three bases that are found in DNA? b. Is cytosine a purine or a pyrimidine? c. Give the name and the one letter abbreviation of the base cytosine base-pairs (H-bonds) with in DNA. d. What is the name of the molecule composed of cytosine linked to ribose? e. Indicate on the drawing through which atom cytosine is connected to ribose or deoxyribose f. Indicate on the drawing which groups on cytosine are involved in base-pairing (H-bonding) with its complementary base

The link between a pyrimidine and ribose is made from the ___ of the pyrimidine ring to the ___ of the ribose. Dropdown 1: N-1, N-3 Dropdown 2: C-1ꞌ, C-2ꞌ, C-3ꞌ, C-4ꞌ, C-5ꞌ

Which of the following bases pairs with guanine?

Genomic DNA that encodes proteins undergoes the process of _____ to produce _____.

In DNA, the ribose derivative lacks an _____ on C-_____.

The results of DNA sequencing are obtained by first separating different-sized pieces of DNA using _____ followed by detection of the particular dideoxynucleotide using _____.

Genomic DNA is _____, resulting in the production of _____.

Which diagram correctly depicts a ribosome engaged in translation?

What enzyme is required to form a new phosphodiester bond when inserting DNA into a plasmid?

The DNA strand that serves as the template for the synthesis of RNA is often called the _____.

What term describes a small, circular molecule of DNA that can be used to transfer genetic material from one organism to another?

The link between a purine and ribose is made from the ____ of the purine ring to the ____ of the ribose. Dropdown 1: N-1, N-3, N-7, N-9 Dropdown 2: C-1ꞌ, C-2ꞌ, C-3ꞌ, C-4ꞌ, C-5ꞌ