Question 1

Which of the following is found in high concentration in all telomeres?

Accepted Answer

A)  A 
B)  C 
C)  G 
D)  T 
E)  pyrimidines 
A)  A 
B)  C 
C)  G 
D)  T 
E)  pyrimidines C

Question 2

Which is true of Klenow fragments?

Accepted Answer

A)  They are the result of removal of the proofreading domain of a prokaryotic DNA polymerase 
B)  They result from treatment of ligase with a protease 
C)  Protease treatment of DNA polymerase III can give rise to Klenow fragments 
D)  They are another name for Okazaki fragments 
E)  An important part of the replisome, they block nucleophilic attack on the phosphodiester backbone. 
A)  They are the result of removal of the proofreading domain of a prokaryotic DNA polymerase 
B)  They result from treatment of ligase with a protease 
C)  Protease treatment of DNA polymerase III can give rise to Klenow fragments 
D)  They are another name for Okazaki fragments 
E)  An important part of the replisome, they block nucleophilic attack on the phosphodiester backbone. A

Question 3

In prokaryotes, the RecBCD recombination pathway requires

Accepted Answer

A)  an 8-base Chi sequence 
B)  binding of RecA to DNA as a first step 
C)  RecBCD to bind to single-strand DNA after DNA nicking 
D)  a primer to be laid down by primase 
E)  all of the above 
A)  an 8-base Chi sequence 
B)  binding of RecA to DNA as a first step 
C)  RecBCD to bind to single-strand DNA after DNA nicking 
D)  a primer to be laid down by primase 
E)  all of the above A

Question 4

The 5' end of a polynucleotide contains _____ while the 3' end contains _____.

Accepted Answer

A)  a hydroxyl group; a phosphate group 
B)  a phosphate group; a hydroxyl group 
C)  a phosphate group; a phosphate group 
D)  a hydroxyl group; a hydroxyl group 
E)  none of the above 
A)  a hydroxyl group; a phosphate group 
B)  a phosphate group; a hydroxyl group 
C)  a phosphate group; a phosphate group 
D)  a hydroxyl group; a hydroxyl group 
E)  none of the above

Question 5

_________ topoisomerases make double-strand breaks in DNA in a controlled fashion.

Accepted Answer

The answer of _________ topoisomerases make double-strand breaks in DNA...

Question 6

The Ter-Tus system is important to

Accepted Answer

A)  formation of the prokaryotic replisome 
B)  termination of prokaryotic replication 
C)  termination of eurkaryotic replication 
D)  separation of DNA strands during replication 
E)  stabilization of separated DNA strands during replication 
A)  formation of the prokaryotic replisome 
B)  termination of prokaryotic replication 
C)  termination of eurkaryotic replication 
D)  separation of DNA strands during replication 
E)  stabilization of separated DNA strands during replication

Question 7

DNA separated at the replication fork is stabilized by ______.

Accepted Answer

The answer of DNA separated at the replication fork is...

Question 8

What type of bond is made between nucleotides?

Accepted Answer

A)  ester 
B)  phosphate ester 
C)  phosphodiester 
D)  glycosidic 
E)  none of the above 
A)  ester 
B)  phosphate ester 
C)  phosphodiester 
D)  glycosidic 
E)  none of the above

Question 9

Which type of DNA repair is used to remove and replace a single modified base that cannot be converted back to the normal base by a direct repair process?

Accepted Answer

A)  base excision repair 
B)  nucleotide excision repair 
C)  mismatch repair 
D)  DNA photolyase 
E)  none of the above 
A)  base excision repair 
B)  nucleotide excision repair 
C)  mismatch repair 
D)  DNA photolyase 
E)  none of the above

Question 10

Use the following diagram to complete the sentences below
  "_____ is the enzyme which catalyzes phosphodiester bond formation on both strands. Processivity of DNA pol is increased when associated with ____. DNA untwisting is accomplished by ______."

Accepted Answer

A)  A, C, B 
B)  A, B, C 
C)  B, C, A 
D)  A, D, B 
E)  A, E, B 
A)  A, C, B 
B)  A, B, C 
C)  B, C, A 
D)  A, D, B 
E)  A, E, B

Question 11

Genes can be moved by _______.

Accepted Answer

The answer of Genes can be moved by _______....

Question 12

What effect does a topoisomerase have upon DNA?

Accepted Answer

A)  denatures DNA 
B)  converts double stranded DNA to single stranded DNA 
C)  changes the supercoiling of DNA 
D)  changes B-form DNA to A-form 
E)  none of the above 
A)  denatures DNA 
B)  converts double stranded DNA to single stranded DNA 
C)  changes the supercoiling of DNA 
D)  changes B-form DNA to A-form 
E)  none of the above

Question 13

E. coli primer synthesis is catalyzed by ______.

Accepted Answer

The answer of E. coli primer synthesis is catalyzed by...

Question 14

Which is NOT true of enzyme processivity?

Accepted Answer

A)  One definition is the number of reactions the enzyme can catalyze before dissociation from substrate dissociation 
B)  A highly processive DNA polymerase would catalyze incorporation of about 10,000 nucleotides 
C)  Processivity is only used to characterize DNA polymerases 
D)  Processivity of DNA polymerases can vary with replicon proteins and enzymes. 
E)  all of the above are true of enzyme processivity 
A)  One definition is the number of reactions the enzyme can catalyze before dissociation from substrate dissociation 
B)  A highly processive DNA polymerase would catalyze incorporation of about 10,000 nucleotides 
C)  Processivity is only used to characterize DNA polymerases 
D)  Processivity of DNA polymerases can vary with replicon proteins and enzymes. 
E)  all of the above are true of enzyme processivity

Question 15

What is the purpose of DNA helicase?

Accepted Answer

A)  removal of supercoiling to allow for replication 
B)  synthesis of RNA primer in front of DNA polymerase 
C)  separation of double stranded DNA into single stranded DNA in front of DNA polymerase 
D)  conversion of single stranded DNA to double stranded DNA behind DNA polymerase 
E)  none of the above 
A)  removal of supercoiling to allow for replication 
B)  synthesis of RNA primer in front of DNA polymerase 
C)  separation of double stranded DNA into single stranded DNA in front of DNA polymerase 
D)  conversion of single stranded DNA to double stranded DNA behind DNA polymerase 
E)  none of the above

Question 16

An E. coli has _____ replication fork(s) on its single chromosome; humans have _____ replication fork(s) on each chromosome.

Accepted Answer

A)  1; 1 
B)  1; 2 
C)  2; 2 
D)  2; many 
E)  many; many 
A)  1; 1 
B)  1; 2 
C)  2; 2 
D)  2; many 
E)  many; many

Question 17

What term is used to describe short segments of DNA that are copied many times and inserted randomly in chromosomes?

Accepted Answer

A)  moderately repetitive sequences 
B)  highly repetitive sequences 
C)  transposable elements 
D)  spliced sequences 
E)  none of the above 
A)  moderately repetitive sequences 
B)  highly repetitive sequences 
C)  transposable elements 
D)  spliced sequences 
E)  none of the above

Question 18

The RNA segment to which DNA residues are added during replication is the ______.

Accepted Answer

The answer of The RNA segment to which DNA residues...

Question 19

What does a reverse transcriptase do?

Accepted Answer

A)  replicates DNA in a 3' to 5'fashion 
B)  replicates RNA in a 5' to 3'fashion 
C)  replicates RNA in a 3' to 5'fashion 
D)  converts a DNA genome into an RNA genome 
E)  converts an RNA genome into a DNA genome 
A)  replicates DNA in a 3' to 5'fashion 
B)  replicates RNA in a 5' to 3'fashion 
C)  replicates RNA in a 3' to 5'fashion 
D)  converts a DNA genome into an RNA genome 
E)  converts an RNA genome into a DNA genome

Question 20

What is the process used for genes shuffling in meiosis that is also used to repair double-strand breaks?

Accepted Answer

A)  homologous end-joining 
B)  nonhomologous end-joining 
C)  recombination 
D)  non-mutagenic strand coupling 
E)  telomerase-assisted ligation 
A)  homologous end-joining 
B)  nonhomologous end-joining 
C)  recombination 
D)  non-mutagenic strand coupling 
E)  telomerase-assisted ligation

Exam 14: Dna Replication, Damage and Repair

Which of the following is found in high concentration in all telomeres?

Which is true of Klenow fragments?

In prokaryotes, the RecBCD recombination pathway requires

The 5' end of a polynucleotide contains _____ while the 3' end contains _____.

_________ topoisomerases make double-strand breaks in DNA in a controlled fashion.

The Ter-Tus system is important to

DNA separated at the replication fork is stabilized by ______.

What type of bond is made between nucleotides?

Which type of DNA repair is used to remove and replace a single modified base that cannot be converted back to the normal base by a direct repair process?

Use the following diagram to complete the sentences below "_____ is the enzyme which catalyzes phosphodiester bond formation on both strands. Processivity of DNA pol is increased when associated with ____. DNA untwisting is accomplished by ______."

Genes can be moved by _______.

What effect does a topoisomerase have upon DNA?

E. coli primer synthesis is catalyzed by ______.

Which is NOT true of enzyme processivity?

What is the purpose of DNA helicase?

An E. coli has _____ replication fork(s) on its single chromosome; humans have _____ replication fork(s) on each chromosome.

What term is used to describe short segments of DNA that are copied many times and inserted randomly in chromosomes?

The RNA segment to which DNA residues are added during replication is the ______.

What does a reverse transcriptase do?

What is the process used for genes shuffling in meiosis that is also used to repair double-strand breaks?

The Chemical Foundations of Biochemistry

Nucleic Acids

Proteins I

Proteins Iienzymes

Membranes and an Introduction to Signal Transduction

Carbohydrates I

The Common Catabolic Pathway

Carbohydrates II

Lipids I

Lipids II

Amine Metabolism

Metabolism Integration

Nucleotide Deoxynucleotide Metabolism

Rna Synthesis

Protein

Control of Gene

Determination of Macromolecular Structure

Allosterism and Receptor-Ligand

Designer Proteins and Protein Folding

Biomolecule Purification

Bioinformatics and Omics

Signal

Protein Trafficking

Photosynthesis Nitrogen

Filters

The 5' end of a polynucleotide contains _ while the 3' end contains _.

Use the following diagram to complete the sentences below "_ is the enzyme which catalyzes phosphodiester bond formation on both strands. Processivity of DNA pol is increased when associated with . DNA untwisting is accomplished by __."

An E. coli has _ replication fork(s) on its single chromosome; humans have _ replication fork(s) on each chromosome.