Exam 16: Genome Analysis: DNA Typing, Genomics, and Beyond

After SNP analysis of a region of the Apolipoprotein E gene, you determine that a patient carries the apoE4 allele. This allele is linked with Alzheimer's disease. Counsel the patient regarding their prognosis.

Describe the process of making a DNA "chip."

Forensic DNA typing often involves recovery of very tiny amounts of DNA from a crime scene. What is a major concern in analyzing this DNA?

The variable number of minisatellite repeats in the human genome primarily results from

In a comparative gene expression analysis between two samples labeled with Cy3 (green) and Cy5 (red), if the two samples contained equivalent amounts of mRNA for a particular gene, the spot on the array corresponding to that gene would appear

What kinds of questions can be better addressed using a protein microarray rather than a DNA microarray?

Following up on reports of a man who attacked an elderly lady (Victim Reference Sample) and spit in her face, the police went to the homes of Suspect 1 and Suspect 2, who each gave police a cheek swab. The electropherogram resulting from STR analysis is shown below. The Evidence Sample is spit from a handkerchief the elderly woman used to wipe her face. Your job is to decide which suspect committed the crime.

Multiplex PCR is

Compare and contrast an analytical protein array with a functional array. What type of information can be obtained from each method?

What is the meaning of the term "synteny" in comparative genomics?

After sequencing the entire genome of a novel organism by the whole-genome shotgun approach, you use a computer program to search for start/stop signals for translation and homology searches based on similarity to cDNAs in the database. You predict approximately 20,000 genes. Is this an accurate representation of the total number of genes in the organism? Why or why not?

Which statement is not correct?

Match each technique for high-throughput analysis of proteins with its possible application(s): Technique Analytical protein array Peptide mass fingerprinting using MALDI-TOF Shotgun proteomics using MS/MS Application Analysis of a single isolated protein from a protein gel Analysis of an entire proteome Analysis of ligand binding