Exam 11: DNA Technology

The following Southern blot was performed to examine a mutation that is linked to a genetic disease.The blot was probed with fluorescent-labeled cDNA of the gene.What is the most likely explanation for the abnormal bands in the patient in comparison with the normal control?

In the following family, I-2 and II-1 have died from a rare form of colon cancer that is inherited as an autosomal dominant trait.The remaining family members have undergone genotyping for a microsatellite marker close to the known gene for colon cancer susceptibility.According to this analysis, what are the genotypes of the children in the third generation (left to right; the term carrier is used for a person who has the genetic disposition but has not yet developed the disease)?

Lane 1 in the following Southern blot contains a sample from a normal control, and lane 2 contains a sample from a patient.The arrow indicates the location of the wells in the agarose gel used in this analysis.The probe used was a complementary DNA (cDNA).Which type of mutation is most likely, on the basis of these data?

PCR and DNA sequencing differ in which of the following requirements?

The binding of an oligonucleotide probe to a specific sequence of DNA is influenced by which of the following:

A virgin was raped in Los Angeles and became pregnant.Police investigations have identified four suspects.The woman decided to go through with the pregnancy for religious reasons.It was possible to isolate a few cells of fetal origin from the maternal blood.Enough DNA was obtained for one polymerase chain reaction (PCR).The results for one microsatellite are shown in the gel image in Figure 11.1.Lane 1 is the mother's DNA, lane 2 is the fetal DNA, and lanes 3 to 6 are the suspect's DNA.Which of the suspects remain suspects after this analysis?

Lane 1 in the following Southern blot contains a sample from a normal control, and lane 2 contains a sample from a patient.The probe is based on the cDNA of a gene.Which type of mutation is most likely, on the basis of these data?

RNA interference can be used for experimental and possibly therapeutic purposes.RNA interference is triggered by:

Many oncogenic mutations change the rate at which cellular genes are expressed.All of the following methods can be used to compare the gene expression profiles of cancer cells with those of the normal cells from which they are derived, except:

Which of the following is the most plausible recognition site for a restriction endonuclease?

In Figure 11.6, the first 10 bases of the DNA strand being sequenced are:

The most important "selectable markers" used in recombinant DNA technology with bacteria are:

What are loxP sites, and for which purpose are they used?

In the following dot blot, the probe used in the upper row is for the normal sequence while the probe in the second row is for the -Phe mutation of the cystic fibrosis gene.What are the genotypes of the four people investigated?

Figure 11.5 is an image of a gel electrophoresis pattern of a microsatellite.The samples are random blood samples from New York, and alleles containing 7, 12, 20, 22, and 35 repeat units can be seen in this gel.Which fragment corresponds to the allele containing 35 repeats?