Question 1

Which of the following analyzers uses radio frequency to detect and differentiate leukocytes?

Accepted Answer

A) Bayer Advia 2120 
B) Abbott Sapphire 
C) Sysmex XE-2100 
D) Coulter Gen-S 
A) Bayer Advia 2120 
B) Abbott Sapphire 
C) Sysmex XE-2100 
D) Coulter Gen-S C

Question 2

Explain the purpose of the following used in the standard hematologic cell-counting detection methods:
a.VCS flow cell
b.Photodetector
c.Sheath fluid
d.Direct current detection block

Accepted Answer

VCS flow cell: to detect each WBC’s volume, conductivity, and scatter for determination of the five part differential
Photodetector: to measure the absorbance of the unknown blood sample passing through it (used in spectrophotometric detection of Hgb)
Sheath fluid: to promote hydrodynamic focusing of cells passing through photodetector
Direct current detection block: to detect electrical resistance when cells pass through the aperture

Question 3

Which of the following is measured immunologically on the Diagnostica Stago STA Compact?

Accepted Answer

A) von Willebrand factor 
B) D-dimer 
C) Lupus anticoagulants 
D) PT and APTT 
A) von Willebrand factor 
B) D-dimer 
C) Lupus anticoagulants 
D) PT and APTT A

Question 4

An analyzer is measuring a clot forming by the interaction of a stationary probe with a moving probe in a continuous motion.Suddenly the moving probe stops,and a clot is seen.Name the analyzer.

Accepted Answer

A) Diagnostica Stago 
B) MDA 180 
C) Trinity Biotech AMAX Destiny Plus 
D) BBL Fibrometer 
A) Diagnostica Stago 
B) MDA 180 
C) Trinity Biotech AMAX Destiny Plus 
D) BBL Fibrometer

Question 5

The immature platelet fraction (IPF)is a parameter derived from the Sysmex XE-2100's RET scattergram.An increased IPF is associated with:

Accepted Answer

A) Aplastic anemia 
B) Pernicious anemia 
C) Acute lymphocytic leukemia 
D) Immune thrombocytopenic purpura 
A) Aplastic anemia 
B) Pernicious anemia 
C) Acute lymphocytic leukemia 
D) Immune thrombocytopenic purpura

Question 6

Hydrodynamic focusing is used in automated cell-counting instruments to:

Accepted Answer

A) Prevent coincidence passage through the detection aperture 
B) Increase the visibility of the cells passing through the aperture 
C) Allow for maximal light absorbance through the cells passing through the aperture 
D) Ensure adequate uptake of fluorescent staining by the cells 
A) Prevent coincidence passage through the detection aperture 
B) Increase the visibility of the cells passing through the aperture 
C) Allow for maximal light absorbance through the cells passing through the aperture 
D) Ensure adequate uptake of fluorescent staining by the cells

Question 7

Name two analytes that can be measured immunologically,and list two instruments that currently use this method.

Accepted Answer

Two analytes: D-dimer and VWF

Question 8

Using the Fibrometer instrument,clot detection occurs when:

Accepted Answer

A) Fibrin strands complete an electric circuit. 
B) Fibrin formation increases light absorbance. 
C) Fibrin strands decrease ball movement in a magnetic field. 
D) Fibrin formation decreases turbidity of the reaction mixture. 
A) Fibrin strands complete an electric circuit. 
B) Fibrin formation increases light absorbance. 
C) Fibrin strands decrease ball movement in a magnetic field. 
D) Fibrin formation decreases turbidity of the reaction mixture.

Question 9

Which of the following analyzers measures reticulocytes by sphering the blood cells prior to analysis?

Accepted Answer

A) Coulter LH series 
B) Coulter Gen-S 
C) Abbott Sapphire 
D) Sysmex XE-2100 
A) Coulter LH series 
B) Coulter Gen-S 
C) Abbott Sapphire 
D) Sysmex XE-2100

Question 10

Using the chromogenic methodology,a coagulation protein's activity level is related to:

Accepted Answer

A) An increase in turbidity 
B) The observed color change 
C) The time needed to convert fibrinogen to fibrin 
D) A decreased viscosity as plasmin digests fibrin 
A) An increase in turbidity 
B) The observed color change 
C) The time needed to convert fibrinogen to fibrin 
D) A decreased viscosity as plasmin digests fibrin

Question 11

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

Accepted Answer

A) 0 degrees 
B) 7 degrees 
C) 90 degrees 
D) 90 degrees depolarized 
A) 0 degrees 
B) 7 degrees 
C) 90 degrees 
D) 90 degrees depolarized

Question 12

Which of the following instruments does not rely on impedance to enumerate cells:

Accepted Answer

A) Advia 120 
B) XE-2100 
C) Sapphire 
D) Coulter 
A) Advia 120 
B) XE-2100 
C) Sapphire 
D) Coulter

Question 13

The purpose of the photodetector on the Diagnostica Stago STA Compact is to:

Accepted Answer

A) Measure an increase in absorbance caused by the interaction of VWF with its monoclonal antibody 
B) Measure an increase in transmittance caused by the interaction of D-dimer fragments in the patient's plasma with its monoclonal antibody 
C) Promote the ball's oscillation movement through the electromagnetic sensors 
D) Measure the amount of cleavage of p-nitroaniline 
A) Measure an increase in absorbance caused by the interaction of VWF with its monoclonal antibody 
B) Measure an increase in transmittance caused by the interaction of D-dimer fragments in the patient's plasma with its monoclonal antibody 
C) Promote the ball's oscillation movement through the electromagnetic sensors 
D) Measure the amount of cleavage of p-nitroaniline

Question 14

Which analyzer has the ability to quantify nucleated RBCs and calculate a corrected WBC count?

Accepted Answer

A) Coulter Gen-S 
B) Sysmex XE-2100 
C) Abbott Cell Dyn Sapphire 
D) Coulter LH 780 
A) Coulter Gen-S 
B) Sysmex XE-2100 
C) Abbott Cell Dyn Sapphire 
D) Coulter LH 780

Question 15

Which technologies are used to generate the LH 750's WBC scatterplot?

Accepted Answer

A) Impedance,light scatter,and conductivity 
B) Fluorescence intensity and radio frequency 
C) Absorbance and conductivity 
D) Light scatter,absorbance,and radio frequency 
A) Impedance,light scatter,and conductivity 
B) Fluorescence intensity and radio frequency 
C) Absorbance and conductivity 
D) Light scatter,absorbance,and radio frequency

Question 16

Cell volume and cell number are required elements for which of the following?

Accepted Answer

A) WBC scatterplots 
B) WBC cytograms 
C) RBC histograms 
D) RBC scattergrams 
A) WBC scatterplots 
B) WBC cytograms 
C) RBC histograms 
D) RBC scattergrams

Question 17

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

Accepted Answer

A) New methylene blue 
B) Propidium iodide 
C) Polymethine 
D) Oxazine 
A) New methylene blue 
B) Propidium iodide 
C) Polymethine 
D) Oxazine

Question 18

The immunologic technique available on the AMAX Destiny Plus utilizes specific 	reagents with:

Accepted Answer

A) Soluble monoclonal antibody 
B) Fluorescent-labeled microparticles 
C) Enzyme-labeled monoclonal antibody 
D) Monoclonal antibody-coated microparticles 
A) Soluble monoclonal antibody 
B) Fluorescent-labeled microparticles 
C) Enzyme-labeled monoclonal antibody 
D) Monoclonal antibody-coated microparticles

Question 19

Choose the correct statement regarding chromogenic analysis in hemostasis testing from the following choices.

Accepted Answer

A) Chromogenic analysis depends on the selected analyte's ability to cleave a chromophore. 
B) Chromogenic analysis is not subject to preanalytical problems because of its mechanism of clot detection. 
C) There is no difference between chromogenic analysis and electromechanical detection. 
D) Chromogenic analysis depends on the optical detection of ball movement through a steel tube as a clot forms. 
A) Chromogenic analysis depends on the selected analyte's ability to cleave a chromophore. 
B) Chromogenic analysis is not subject to preanalytical problems because of its mechanism of clot detection. 
C) There is no difference between chromogenic analysis and electromechanical detection. 
D) Chromogenic analysis depends on the optical detection of ball movement through a steel tube as a clot forms.

Question 20

Which of the following parameters are calculated on the Coulter Gen-S?

Accepted Answer

A) Hemoglobin,MCH,MCHC 
B) Hematocrit,MCH,MCHC 
C) Absolute neutrophil count,RBC count,platelet count 
D) RBC count,hematocrit,MCH 
A) Hemoglobin,MCH,MCHC 
B) Hematocrit,MCH,MCHC 
C) Absolute neutrophil count,RBC count,platelet count 
D) RBC count,hematocrit,MCH

Exam 39: Automation in Hematology

Which of the following analyzers uses radio frequency to detect and differentiate leukocytes?

Explain the purpose of the following used in the standard hematologic cell-counting detection methods: a.VCS flow cell b.Photodetector c.Sheath fluid d.Direct current detection block

Which of the following is measured immunologically on the Diagnostica Stago STA Compact?

An analyzer is measuring a clot forming by the interaction of a stationary probe with a moving probe in a continuous motion.Suddenly the moving probe stops,and a clot is seen.Name the analyzer.

The immature platelet fraction (IPF)is a parameter derived from the Sysmex XE-2100's RET scattergram.An increased IPF is associated with:

Hydrodynamic focusing is used in automated cell-counting instruments to:

Name two analytes that can be measured immunologically,and list two instruments that currently use this method.

Using the Fibrometer instrument,clot detection occurs when:

Which of the following analyzers measures reticulocytes by sphering the blood cells prior to analysis?

Using the chromogenic methodology,a coagulation protein's activity level is related to:

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

Which of the following instruments does not rely on impedance to enumerate cells:

The purpose of the photodetector on the Diagnostica Stago STA Compact is to:

Which analyzer has the ability to quantify nucleated RBCs and calculate a corrected WBC count?

Which technologies are used to generate the LH 750's WBC scatterplot?

Cell volume and cell number are required elements for which of the following?

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

The immunologic technique available on the AMAX Destiny Plus utilizes specific reagents with:

Choose the correct statement regarding chromogenic analysis in hemostasis testing from the following choices.

Which of the following parameters are calculated on the Coulter Gen-S?

Introduction

Cellular Homeostasis

Structure and Function of Hematopoietic Organs

Hematopoiesis

The Erythrocyte

Hemoglobin

Granulocytes and Monocytes

Lymphocytes

The Platelet

The Complete Blood Count and Peripheral Blood Smear Evaluation

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopathies: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Hypoproliferative Anemias

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Disorders of Leukocytes: Granulocytes and Monocytes

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Precursor Lymphoid Neoplasms

Mature Lymphoid Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology Laboratory

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hemostasis: Laboratory Testing and Instrumentation

Hematology Procedures

Bone Marrow Examination

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

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