Exam 12: Microbial Biotechnology

The preferred fuel of choice for the original Model T Ford was gasoline.

Tolerance to Roundup in crop plants relies on:

The EPSP (5-enolpyruvylshikimate 3-phosphate)gene that permitted crop plants to develop Roundup tolerance was first introduced into:

For optimal expression of a eukaryal gene in bacteria,the expression vector must contain all of the following except one.Which would it NOT include?

To what are the yellow regions of pTi analogous?

The CRISPR-Cas DNA editing system relies upon: (Select all that apply)

Why is a beta galactosidase fusion protein produced as part of recombinant insulin production?

For optimal expression of a eukaryal gene in a eukaryal cell,the expression vector must contain all of the following except one.Which would it NOT include?

Why are the opine synthesis genes considered expendible in a pTi vector for transgenic plant creation? (Select all that apply)

Which of these would be an example of a "primary metabolite" fermentation product?

What essential principle of PCR appears to have been overlooked in the figure depicting Part B (PCR-based site directed mutagenesis)?

Insect resistant transgenic plants are produced by the insertion of a gene into the plant genome from which insect toxin-producing bacterium?

Expression of eukaryal DNA in bacterial cells requires the insertion of _______ into the plasmid expression vector as the structural component of the gene.

Which modification might best produce cloned thermostable enzymes for industrial applications?

Millions of tons of this amino acid,commonly used as a flavor enhancer,are produced annually.

Use of the Ti plasmid (pTi)binary vector system to create recombinant pants requires retention in the T-DNA of: (Select all that apply)

Which of these proteins would be useful in experiments designed to use a DNA shuffling approach for directed evolution?

What purposes may secondary metabolites serve? (Select all that apply)

Some types of anti-cholesterol drugs,such as the statins,are produced by microbes.

PCR can be used to introduce mutations into a microbial genome.