Question 1

The EPSP (5-enolpyruvylshikimate 3-phosphate)gene that permitted crop plants to develop Roundup tolerance originated in:

Accepted Answer

A) petunias 
B) bacteria 
C) lepidoptera 
D) chrysids 
E) viruses 
A) petunias 
B) bacteria 
C) lepidoptera 
D) chrysids 
E) viruses

Question 2

Which of these utilizes "error-prone polymerase chain reaction"?

Accepted Answer

A) site-directed mutagenesis 
B) directed enzyme evolution 
C) random mutagenesis of the genome 
D) gene linkage experiments 
E) gene regulation experiments 
A) site-directed mutagenesis 
B) directed enzyme evolution 
C) random mutagenesis of the genome 
D) gene linkage experiments 
E) gene regulation experiments

Question 3

Which primary fraction of plant biomass is used as feedstock for biofuels production?

Accepted Answer

A) disaccharides 
B) cellulose 
C) pectin 
D) chitin 
E) protein 
A) disaccharides 
B) cellulose 
C) pectin 
D) chitin 
E) protein

Question 4

Polyhydroxyalkanoates are associated with the production of this material.

Accepted Answer

A) ethanol 
B) methane 
C) biodiesel 
D) butanol 
E) bioplastics 
A) ethanol 
B) methane 
C) biodiesel 
D) butanol 
E) bioplastics

Question 5

Why is an affinity tag fusion protein produced?

Accepted Answer

A) To increase the biological activity of the protein. 
B) To increase overall expression of the cloned gene. 
C) To facilitate the purification of the recombinant protein. 
D) To ensure correct transcription termination. 
E) To ensure that translation starts correctly. 
A) To increase the biological activity of the protein. 
B) To increase overall expression of the cloned gene. 
C) To facilitate the purification of the recombinant protein. 
D) To ensure correct transcription termination. 
E) To ensure that translation starts correctly.

Question 6

The introduction of DNA into plants for the creation of transgenic plants often uses a plasmid mediated process from which bacterium?

Accepted Answer

A) Rhizobium legmuninosarum 
B) Pseudomonas syringae 
C) Corynebacterium glutamicum 
D) Xanthomonas campestris 
E) Agrobacterium tumefaciens 
A) Rhizobium legmuninosarum 
B) Pseudomonas syringae 
C) Corynebacterium glutamicum 
D) Xanthomonas campestris 
E) Agrobacterium tumefaciens

Question 7

The main advantage of using microorganisms for the production of amino acids over chemical synthesis is …

Accepted Answer

A) cost. 
B) stereospecificity. 
C) increased production yield. 
D) ease of purification. 
E) a single microbe will produce all 20 amino acids. 
A) cost. 
B) stereospecificity. 
C) increased production yield. 
D) ease of purification. 
E) a single microbe will produce all 20 amino acids.

Question 8

What vitamin is used to treat pernicious anemia?

Accepted Answer

A) C 
B) B₁ 
C) B₁₂ 
D) niacin 
E) B₆ 
A) C 
B) B₁ 
C) B₁₂ 
D) niacin 
E) B₆

Question 9

Why is the use of cyanobacteria for the production of bioplastics an attractive commercial investment?

Accepted Answer

A) Their PHA are stronger than those found in other bacteria. 
B) The cost of production is less. 
C) Their bioplastics are lighter than other types. 
D) Their bioplastics are more pliable. 
E) The PHA produced are easier to work with than other types. 
A) Their PHA are stronger than those found in other bacteria. 
B) The cost of production is less. 
C) Their bioplastics are lighter than other types. 
D) Their bioplastics are more pliable. 
E) The PHA produced are easier to work with than other types.

Question 10

Although plant biomass is plentiful for use as biofuel,which major problem needs to be overcome?

Accepted Answer

A) Increasing the efficiency of breakdown of cellulose into glucose. 
B) Decreasing the cost of production of plant biomass. 
C) Increasing the production of cars that can run on ethanol. 
D) Polluting by-products of the conversion of glucose to ethanol. 
E) Increasing use of biofuels may drive crop prices down. 
A) Increasing the efficiency of breakdown of cellulose into glucose. 
B) Decreasing the cost of production of plant biomass. 
C) Increasing the production of cars that can run on ethanol. 
D) Polluting by-products of the conversion of glucose to ethanol. 
E) Increasing use of biofuels may drive crop prices down.

Question 11

Why may an affinity tag fusion protein be produced? (Select all that apply)

Accepted Answer

A) To increase the biological activity of the protein. 
B) To facilitate location intracellularly or intra-organismally of the recombinant protein. 
C) To facilitate the purification of the recombinant protein. 
D) To ensure correct transcription termination. 
E) To ensure that translation starts correctly. 
A) To increase the biological activity of the protein. 
B) To facilitate location intracellularly or intra-organismally of the recombinant protein. 
C) To facilitate the purification of the recombinant protein. 
D) To ensure correct transcription termination. 
E) To ensure that translation starts correctly.

Question 12

Why might butanol be a better biofuel than ethanol?

Accepted Answer

A) Butanol has a higher octane rating. 
B) Butanol is easier to produce. 
C) Butanol contains more energy per unit volume. 
D) Butanol can be produced from cheaper feedstocks. 
E) Butanol costs less to produce. 
A) Butanol has a higher octane rating. 
B) Butanol is easier to produce. 
C) Butanol contains more energy per unit volume. 
D) Butanol can be produced from cheaper feedstocks. 
E) Butanol costs less to produce.

Question 13

Which was the first recombinant human protein produced and marketed commercially?

Accepted Answer

A) insulin 
B) human growth hormone 
C) recombinant factor VIII 
D) cytochrome c oxidase 
E) thyroid hormone 
A) insulin 
B) human growth hormone 
C) recombinant factor VIII 
D) cytochrome c oxidase 
E) thyroid hormone

Question 14

Which of these would be an example of a "secondary metabolite" fermentation product?

Accepted Answer

A) ethanol 
B) acetone 
C) butanol 
D) penicillin 
E) propanol 
A) ethanol 
B) acetone 
C) butanol 
D) penicillin 
E) propanol

Question 15

In creating Roundup- ready transgenic plants,the petunia EPSP gene product provided:

Accepted Answer

A) gene sequences to assist in homologous recombination events 
B) a leader sequence likely to facilitate organellar transport of the desired gene product 
C) genetic material to be inserted into the Ti plasmid 
D) the first transgenic crop plant DNA donor 
E) none is a correct choice 
A) gene sequences to assist in homologous recombination events 
B) a leader sequence likely to facilitate organellar transport of the desired gene product 
C) genetic material to be inserted into the Ti plasmid 
D) the first transgenic crop plant DNA donor 
E) none is a correct choice

Question 16

Statins work to reduce cholesterol levels in humans by:

Accepted Answer

A) competitively inhibiting HMG-coA reductaseenzyme 
B) non-competitively inhibiting HMG-coA reductaseenzyme 
C) preventing transcription of HMG-coA reductasemRNA 
D) preventing translation of HMG-coA reductaseenzyme 
E) activating HMG-coA reductaseenzyme 
A) competitively inhibiting HMG-coA reductaseenzyme 
B) non-competitively inhibiting HMG-coA reductaseenzyme 
C) preventing transcription of HMG-coA reductasemRNA 
D) preventing translation of HMG-coA reductaseenzyme 
E) activating HMG-coA reductaseenzyme

Question 17

Which of these proteins could theoretically be mutated in order to create novel site specific DNA binding proteins with single strand endonuclease activity? (Select all that apply)

Accepted Answer

A) Restriction endonucleases 
B) Zinc finger nucleases 
C) Repressor proteins 
D) Inducer molecules 
E) Transcription activator-like effector nucleases 
A) Restriction endonucleases 
B) Zinc finger nucleases 
C) Repressor proteins 
D) Inducer molecules 
E) Transcription activator-like effector nucleases

Question 18

Which of the following methods have been used to increase product production by an industrial microbial strain?

Accepted Answer

A) site-directed mutagenesis 
B) random mutagenesis 
C) transformation 
D) transduction 
E) all of these choices have been used. 
A) site-directed mutagenesis 
B) random mutagenesis 
C) transformation 
D) transduction 
E) all of these choices have been used.

Question 19

Random mutagenesis is often used in industry to produce microbial strains that have increased production ability.

Accepted Answer

A) True 
 B)False

Question 20

Under what condition would directed enzyme evolution be more appropriate that site-directed mutagenesis?

Accepted Answer

A) When attempting to produce an altered enzyme. 
B) When you want the enzyme to catalyze a different type of reaction. 
C) If very little is known about the enzyme structure. 
D) If you want to permanently disable the enzyme. 
E) If you want the enzyme expressed in a different organism. 
A) When attempting to produce an altered enzyme. 
B) When you want the enzyme to catalyze a different type of reaction. 
C) If very little is known about the enzyme structure. 
D) If you want to permanently disable the enzyme. 
E) If you want the enzyme expressed in a different organism.

Exam 12: Microbial Biotechnology

The EPSP (5-enolpyruvylshikimate 3-phosphate)gene that permitted crop plants to develop Roundup tolerance originated in:

Which of these utilizes "error-prone polymerase chain reaction"?

Which primary fraction of plant biomass is used as feedstock for biofuels production?

Polyhydroxyalkanoates are associated with the production of this material.

Why is an affinity tag fusion protein produced?

The introduction of DNA into plants for the creation of transgenic plants often uses a plasmid mediated process from which bacterium?

The main advantage of using microorganisms for the production of amino acids over chemical synthesis is …

What vitamin is used to treat pernicious anemia?

Why is the use of cyanobacteria for the production of bioplastics an attractive commercial investment?

Although plant biomass is plentiful for use as biofuel,which major problem needs to be overcome?

Why may an affinity tag fusion protein be produced? (Select all that apply)

Why might butanol be a better biofuel than ethanol?

Which was the first recombinant human protein produced and marketed commercially?

Which of these would be an example of a "secondary metabolite" fermentation product?

In creating Roundup- ready transgenic plants,the petunia EPSP gene product provided:

Statins work to reduce cholesterol levels in humans by:

Which of these proteins could theoretically be mutated in order to create novel site specific DNA binding proteins with single strand endonuclease activity? (Select all that apply)

Which of the following methods have been used to increase product production by an industrial microbial strain?

Random mutagenesis is often used in industry to produce microbial strains that have increased production ability.

Under what condition would directed enzyme evolution be more appropriate that site-directed mutagenesis?

The Microbial World

Bacteria

Eukaryal Microbes

Archaea

Viruses

Cultivating Microorganisms

DNA Replication and Gene Expression

Viral Replication Strategies

Bacterial Genetic Analysis

Microbial Genomics

Regulation of Gene Expression

Metabolism

Biogeochemical Cycles

Microbial Ecosystems

The Microbiology of Food and Water

Microbial Symbionts

Introduction to Infectious Diseases

Innate Host Defenses Against Microbial Invasion

Adaptive Immunity

Virulence Factors and Pathogenic Mechanisms of Bacterial Infections

Viral Pathogenesis

Eukaryal Microbe Pathogenesis

Control of Infectious Diseases

Filters