Question 1

Which of these amino acids are commonly used as supplements in animal feed to promote growth?

Accepted Answer

A)  glycine and glutamate 
B)  methionine and lysine 
C)  proline and valine 
D)  tryptophan and tyrosine 
E)  aspartate and histidine 
A)  glycine and glutamate 
B)  methionine and lysine 
C)  proline and valine 
D)  tryptophan and tyrosine 
E)  aspartate and histidine

Question 2

Which of these would be an example of a "secondary metabolite" fermentation product?

Accepted Answer

A)  ethanol 
B)  acetone 
C)  butanol 
D)  penicillin 
E)  propanol 
A)  ethanol 
B)  acetone 
C)  butanol 
D)  penicillin 
E)  propanol

Question 3

Although plant biomass is plentiful for use as biofuel,which major problem needs to be overcome?

Accepted Answer

A)  Increasing the efficiency of breakdown of cellulose into glucose. 
B)  Decreasing the cost of production of plant biomass. 
C)  Increasing the production of cars that can run on ethanol. 
D)  Polluting by-products of the conversion of glucose to ethanol. 
E)  Increasing use of biofuels may drive crop prices down. 
A)  Increasing the efficiency of breakdown of cellulose into glucose. 
B)  Decreasing the cost of production of plant biomass. 
C)  Increasing the production of cars that can run on ethanol. 
D)  Polluting by-products of the conversion of glucose to ethanol. 
E)  Increasing use of biofuels may drive crop prices down.

Question 4

Expression of eukaryal DNA in bacterial cells requires the insertion of _______ into the plasmid expression vector.

Accepted Answer

The answer of Expression of eukaryal DNA in bacterial cells...

Question 5

What are the advantages of using microbial enzymes for the synthesis of certain chemicals over conventional chemical synthesis?

Accepted Answer

Microbial enzymes offer several advantag

Question 6

The preferred fuel of choice for the original Model T Ford was gasoline.

Accepted Answer

A) True 
 B)False

Question 7

The main advantage of using microorganisms for the production of amino acids over chemical synthesis is …

Accepted Answer

A)  cost. 
B)  stereospecificity. 
C)  increased production yield. 
D)  ease of purification. 
E)  a single microbe will produce all 20 amino acids. 
A)  cost. 
B)  stereospecificity. 
C)  increased production yield. 
D)  ease of purification. 
E)  a single microbe will produce all 20 amino acids.

Question 8

The introduction of DNA into plants for the creation of transgenic plants often uses a plasmid mediated process from which bacterium?

Accepted Answer

A)  Rhizobium legmuninosarum 
B)  Pseudomonas syringae 
C)  Corynebacterium glutamicum 
D)  Xanthomonas campestris 
E)  Agrobacterium tumefaciens 
A)  Rhizobium legmuninosarum 
B)  Pseudomonas syringae 
C)  Corynebacterium glutamicum 
D)  Xanthomonas campestris 
E)  Agrobacterium tumefaciens

Question 9

For optimal expression of a eukaryal gene in bacteria,the expression vector must contain all of the following except one.Which would it NOT include?

Accepted Answer

A)  a bacterial promoter 
B)  a bacterial termination sequence 
C)  a bacterial ribosomal binding site 
D)  a protein to remove the introns 
E)  a regulatable promoter 
A)  a bacterial promoter 
B)  a bacterial termination sequence 
C)  a bacterial ribosomal binding site 
D)  a protein to remove the introns 
E)  a regulatable promoter

Question 10

Cyanobacteria are known to accumulate polyhydroxyalkanoates (PHA).

Accepted Answer

A) True 
 B)False

Question 11

What vitamin is used to treat pernicious anemia?

Accepted Answer

A)  C 
B)  B₁ 
C)  B₁₂ 
D)  niacin 
E)  B₆ 
A)  C 
B)  B₁ 
C)  B₁₂ 
D)  niacin 
E)  B₆

Question 12

What does the term fermentation refer to in an industrial microbiology setting?

Accepted Answer

A)  The anaerobic growth of microbes. 
B)  The production of acids and alcohols. 
C)  The production of all fermentation products. 
D)  The mass culturing of microbes for the production of a product. 
E)  A low energy yielding catabolic process. 
A)  The anaerobic growth of microbes. 
B)  The production of acids and alcohols. 
C)  The production of all fermentation products. 
D)  The mass culturing of microbes for the production of a product. 
E)  A low energy yielding catabolic process.

Question 13

Bioprospecting is the search for microorganisms that possess useful features for commercial applications.

Accepted Answer

A) True 
 B)False

Question 14

Why are bioplastics often preferred over conventional plastics?

Accepted Answer

A)  Bioplastics are stronger. 
B)  Bioplastics are less dense. 
C)  Bioplastics are biodegradable. 
D)  Bioplastics are cheaper to produce. 
E)  Bioplastics are more pliable. 
A)  Bioplastics are stronger. 
B)  Bioplastics are less dense. 
C)  Bioplastics are biodegradable. 
D)  Bioplastics are cheaper to produce. 
E)  Bioplastics are more pliable.

Question 15

Amylase enzymes followed by glucose isomerase are used to make which sweetener?

Accepted Answer

A)  NutraSweet™ (aspartame) 
B)  high fructose corn syrup 
C)  saccharin 
D)  sucrose 
E)  Splenda™ (sucralose) 
A)  NutraSweet™ (aspartame) 
B)  high fructose corn syrup 
C)  saccharin 
D)  sucrose 
E)  Splenda™ (sucralose)

Question 16

Why is the use of cyanobacteria for the production of bioplastics is an attractive commercial investment?

Accepted Answer

A)  Their PHA are stronger than those found in other bacteria. 
B)  The cost of production is less. 
C)  Their bioplastics are lighter than other types. 
D)  Their bioplastics are more pliable. 
E)  The PHA produced are easier to work with than other types. 
A)  Their PHA are stronger than those found in other bacteria. 
B)  The cost of production is less. 
C)  Their bioplastics are lighter than other types. 
D)  Their bioplastics are more pliable. 
E)  The PHA produced are easier to work with than other types.

Question 17

A his-tag or strep-tag is part of the cloning area in expression vectors.How do these sequences function?

Accepted Answer

A)  To increase gene expression from the cloned DNA. 
B)  In regulation of expression of the cloned DNA. 
C)  As unique restriction enzyme sites to aid in cloning DNA. 
D)  Affinity sequences to aid in purification of the recombinant protein. 
E)  As signal sequences for the secretion of the recombinant protein. 
A)  To increase gene expression from the cloned DNA. 
B)  In regulation of expression of the cloned DNA. 
C)  As unique restriction enzyme sites to aid in cloning DNA. 
D)  Affinity sequences to aid in purification of the recombinant protein. 
E)  As signal sequences for the secretion of the recombinant protein.

Question 18

In industry,fermentation must be done anaerobically.

Accepted Answer

A) True 
 B)False

Question 19

Affinity tags often used in modern expression vectors to produce a fusion protein from the gene of interest,aids in recombinant protein purification.

Accepted Answer

A) True 
 B)False

Question 20

Current biofuel research is focused on the use of microalgae for the production of this material.

Accepted Answer

A)  ethanol 
B)  methanol 
C)  biodiesel 
D)  cellulase 
E)  methane 
A)  ethanol 
B)  methanol 
C)  biodiesel 
D)  cellulase 
E)  methane

Exam 12: Microbial Biotechnology

Which of these amino acids are commonly used as supplements in animal feed to promote growth?

Which of these would be an example of a "secondary metabolite" fermentation product?

Although plant biomass is plentiful for use as biofuel,which major problem needs to be overcome?

Expression of eukaryal DNA in bacterial cells requires the insertion of _______ into the plasmid expression vector.

What are the advantages of using microbial enzymes for the synthesis of certain chemicals over conventional chemical synthesis?

The preferred fuel of choice for the original Model T Ford was gasoline.

The main advantage of using microorganisms for the production of amino acids over chemical synthesis is …

The introduction of DNA into plants for the creation of transgenic plants often uses a plasmid mediated process from which bacterium?

For optimal expression of a eukaryal gene in bacteria,the expression vector must contain all of the following except one.Which would it NOT include?

Cyanobacteria are known to accumulate polyhydroxyalkanoates (PHA).

What vitamin is used to treat pernicious anemia?

What does the term fermentation refer to in an industrial microbiology setting?

Bioprospecting is the search for microorganisms that possess useful features for commercial applications.

Why are bioplastics often preferred over conventional plastics?

Amylase enzymes followed by glucose isomerase are used to make which sweetener?

Why is the use of cyanobacteria for the production of bioplastics is an attractive commercial investment?

A his-tag or strep-tag is part of the cloning area in expression vectors.How do these sequences function?

In industry,fermentation must be done anaerobically.

Affinity tags often used in modern expression vectors to produce a fusion protein from the gene of interest,aids in recombinant protein purification.

Current biofuel research is focused on the use of microalgae for the production of this material.

The Microbial World

Bacteria

Eukaryal Microbes

Archaea

Viruses

Cultivating Microorganisms

DNA Replication and Gene Expression

Viral Replication Strategies

Bacterial Genetic Analysis

Microbial Genomics

Regulation of Gene Expression

Metabolism

Biogeochemical Cycles

Microbial Ecosystems

The Microbiology of Food and Water

Microbial Symbionts

Introduction to Infectious Diseases

Innate Host Defenses Against Microbial Invasion

Adaptive Immunity

Survival in the Host

Viral Pathogenesis

Eukaryal Microbe Pathogenesis

Control of Infectious Diseases

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