Question 1

Which of the following agents lacks a cell wall and is the smallest known free-living form?

Accepted Answer

A)  Chlamydia 
B)  Mycoplasma 
C)  Rickettsia 
D)  Coxiella 
A)  Chlamydia 
B)  Mycoplasma 
C)  Rickettsia 
D)  Coxiella B

Question 2

Media that can be used for the isolation of Mycoplasma include:

Accepted Answer

A)  Thayer-Martin agar with vitamin K. 
B)  5% sheep blood and IsoVitaleX. 
C)  beef or soybean protein with serum and fresh yeast extract. 
D)  hemin and nicotinamide adenine dinucleotide (NA
D)  supplements. 
A)  Thayer-Martin agar with vitamin K. 
B)  5% sheep blood and IsoVitaleX. 
C)  beef or soybean protein with serum and fresh yeast extract. 
D)  hemin and nicotinamide adenine dinucleotide (NA
D)  supplements. C

Question 3

The definitive identification of which of the following organisms is accomplished by hemadsorption techniques?

Accepted Answer

A)  Chlamydia trachomatis 
B)  Mycoplasma hominis 
C)  Mycoplasma pneumoniae 
D)  Mycoplasma genitalium 
A)  Chlamydia trachomatis 
B)  Mycoplasma hominis 
C)  Mycoplasma pneumoniae 
D)  Mycoplasma genitalium C

Question 4

The natural habitat of Mycoplasma spp., which causes infectious disease in humans, can best be described as the:

Accepted Answer

A)  mucous membranes of humans. 
B)  aquatic environment. 
C)  natural environment. 
D)  genitourinary tract of animals. 
A)  mucous membranes of humans. 
B)  aquatic environment. 
C)  natural environment. 
D)  genitourinary tract of animals.

Question 5

Caution should be taken when culturing blood in suspected cases of Mycoplasma infections since the organism is inhibited by which anticoagulant commonly found in commercial blood culture media?

Accepted Answer

A)  Ethylenediaminetetraacetic acid (EDT
A)  
B)  Sodium polyanethol sulfonate (SPS) 
C)  Heparin 
D)  Sodium citrate 
A)  Ethylenediaminetetraacetic acid (EDT
A)  
B)  Sodium polyanethol sulfonate (SPS) 
C)  Heparin 
D)  Sodium citrate

Question 6

Which of the following Mycoplasma species is associated with individuals who are positive for human immunodeficiency virus (HIV), is capable of invading human cells, and can modulate the immune system?

Accepted Answer

A)  M. pneumoniae 
B)  M. genitalium 
C)  M. hominis 
D)  M. fermentans 
A)  M. pneumoniae 
B)  M. genitalium 
C)  M. hominis 
D)  M. fermentans

Question 7

Incorporation of urea into media is used to enhance the detection of which organism?

Accepted Answer

A)  Ureaplasma urealyticum 
B)  Mycoplasma pneumonia 
C)  M. hominis 
D)  M. genitalium 
A)  Ureaplasma urealyticum 
B)  Mycoplasma pneumonia 
C)  M. hominis 
D)  M. genitalium

Question 8

Quality control is set up to test urea agar.M.genitalium and M.hominis are used; however, both organisms are negative for urease activity.Which one of the following statements explains these findings?

Accepted Answer

A)  The urea agar is most likely expired. 
B)  The incubation time is most likely abbreviated. 
C)  A technical error has occurred. 
D)  These two organisms are poor choices for quality control of urea agar. 
A)  The urea agar is most likely expired. 
B)  The incubation time is most likely abbreviated. 
C)  A technical error has occurred. 
D)  These two organisms are poor choices for quality control of urea agar.

Question 9

The laboratory method of choice to identify Mycoplasma and Ureaplasma infections is:

Accepted Answer

A)  enzyme-linked immunosorbent assay (ELIS
A) . 
B)  direct fluorescent antibody (DF
A) . 
C)  latex agglutination. 
D)  real-time polymerase chain reaction (PCR). 
A)  enzyme-linked immunosorbent assay (ELIS
A) . 
B)  direct fluorescent antibody (DF
A) . 
C)  latex agglutination. 
D)  real-time polymerase chain reaction (PCR).

Question 10

Which group of drugs is effective against Mycoplasma pneumoniae infections?

Accepted Answer

A)  Beta-lactams 
B)  Sulfonamides 
C)  Cephalosporins 
D)  Fluoroquinolones 
A)  Beta-lactams 
B)  Sulfonamides 
C)  Cephalosporins 
D)  Fluoroquinolones

Exam 44: Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

Which of the following agents lacks a cell wall and is the smallest known free-living form?

Media that can be used for the isolation of Mycoplasma include:

The definitive identification of which of the following organisms is accomplished by hemadsorption techniques?

The natural habitat of Mycoplasma spp., which causes infectious disease in humans, can best be described as the:

Caution should be taken when culturing blood in suspected cases of Mycoplasma infections since the organism is inhibited by which anticoagulant commonly found in commercial blood culture media?

Which of the following Mycoplasma species is associated with individuals who are positive for human immunodeficiency virus (HIV), is capable of invading human cells, and can modulate the immune system?

Incorporation of urea into media is used to enhance the detection of which organism?

Quality control is set up to test urea agar.M.genitalium and M.hominis are used; however, both organisms are negative for urease activity.Which one of the following statements explains these findings?

The laboratory method of choice to identify Mycoplasma and Ureaplasma infections is:

Which group of drugs is effective against Mycoplasma pneumoniae infections?

Microbial Taxonomy

Bacterial Genetics, Metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action and Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus Micrococcus and Similar Organisms

Streptococcus Enterococcus and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix Lactobacillus and Similar Organisms

Nocardia Streptomyces Rhodococcus and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Chromobacterium, and Related Organisms

Sphingomonas Paucimobilis and Similar Organisms

Moraxella and Related Organisms

Eikenella and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Aggregatibacter, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis, Bordetella Parapertussis, and Related Species

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Overview of Anaerobic Organisms

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections: Overview

Intestinal Protozoa

Blood and Tissue Protozoa

Other Protozoa

Intestinal Nematodes Roundworms

Tissue Nematodes Roundworms

Blood and Tissue Filarialnematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Mucorales Zygomycetes, Dermatophytes, and Opportunitistic and Systemic Mycoses

Dematiaceous Melanizedmolds

Opportunistic Atypical Fungus: Pneumocystis Jiroveci

The Yeasts

Anti-fungal Susceptibility Testing, Therapy, and Prevention

Overview of the Methods and Strategies in Virology

Viruses in Human Disease

Antiviral Therapy, Susceptibility Testing, and Prevention

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis, Encephalitis, and Other Infections of the Central Nervous System