Question 1

Which assay is the current recommended method of detection in most laboratories for influenzas A and B viruses?

Accepted Answer

A)  Conventional cell culture 
B)  Enzyme immunoassays 
C)  Reverse transcriptase polymerase chain reaction (RT-PCR) 
D)  Shell vial culture 
A)  Conventional cell culture 
B)  Enzyme immunoassays 
C)  Reverse transcriptase polymerase chain reaction (RT-PCR) 
D)  Shell vial culture

Question 2

Match the virus with its corresponding property.
-HEp-2 culture cells

Accepted Answer

A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B 
A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B

Question 3

Match the virus with the appropriate specimen.
-Vesicular

Accepted Answer

A) Adenovirus 
B) HSV 
C) HBV 
D) Arbovirus 
A) Adenovirus 
B) HSV 
C) HBV 
D) Arbovirus

Question 4

Some viruses have the ability to stimulate uncontrolled growth of the host cells, resulting in cancer.Which virus has oncogenic properties?

Accepted Answer

A)  Measles 
B)  Papilloma 
C)  Retroviruses 
D)  Influenza 
A)  Measles 
B)  Papilloma 
C)  Retroviruses 
D)  Influenza

Question 5

Match the virus with the appropriate specimen.
-Cerebrospinal fluid (CSF)

Accepted Answer

A) Adenovirus 
B) HSV 
C) HBV 
D) Arbovirus 
A) Adenovirus 
B) HSV 
C) HBV 
D) Arbovirus

Question 6

The function of the glycoprotein spike on some viruses is to:

Accepted Answer

A)  allow viral entry into the host cell. 
B)  protect the nucleic acid. 
C)  prevent drying out and the destruction of the virus. 
D)  stabilize attachment for the lipid envelope. 
A)  allow viral entry into the host cell. 
B)  protect the nucleic acid. 
C)  prevent drying out and the destruction of the virus. 
D)  stabilize attachment for the lipid envelope.

Question 7

Match the virus with its corresponding property.
-R-Mix cells

Accepted Answer

A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B 
A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B

Question 8

Match the virus with its corresponding property.
-Hemadsorption

Accepted Answer

A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B 
A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B

Question 9

The specimen of choice to collect in suspected cases of respiratory syncytial virus (RSV)is:

Accepted Answer

A)  buccal mucosa. 
B)  sputum. 
C)  throat. 
D)  nasopharyngeal. 
A)  buccal mucosa. 
B)  sputum. 
C)  throat. 
D)  nasopharyngeal.

Question 10

The most used EIA for antigen detection that uses a solid phase and membrane is used for detecting which virus?

Accepted Answer

A)  Influenza 
B)  Adenovirus 
C)  HSV 
D)  Respiratory syncytial virus (RSV) 
A)  Influenza 
B)  Adenovirus 
C)  HSV 
D)  Respiratory syncytial virus (RSV)

Question 11

A 2-year-old girl is suspected of having a rotavirus infection.The specimen of choice to collect to identify the agent is:

Accepted Answer

A)  stool. 
B)  blood. 
C)  urine. 
D)  throat. 
A)  stool. 
B)  blood. 
C)  urine. 
D)  throat.

Question 12

Match the virus with the appropriate specimen.
-Blood

Accepted Answer

A) Adenovirus 
B) HSV 
C) HBV 
D) Arbovirus 
A) Adenovirus 
B) HSV 
C) HBV 
D) Arbovirus

Question 13

The cytomegalovirus (CMV)produces cytopathic effects (CPE)in diploid fibroblast cells in an average of 7 days.The results are available within 16 hours using which type of assay?

Accepted Answer

A)  Conventional cell culture 
B)  Shell vial 
C)  Paired sera 
D)  Western blot 
A)  Conventional cell culture 
B)  Shell vial 
C)  Paired sera 
D)  Western blot

Question 14

Match the virus with its corresponding property.
-Adenovirus serotypes 40 and 41

Accepted Answer

A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B 
A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B

Question 15

MATCHING
Match the virus with the specimen in which it is most commonly isolated.
-Genital

Accepted Answer

A) Varicella zoster virus 
B) Enterovirus 
C) Human papilloma virus (HPV) 
D) Parainfluenza virus 
E) CMV 
A) Varicella zoster virus 
B) Enterovirus 
C) Human papilloma virus (HPV) 
D) Parainfluenza virus 
E) CMV

Question 16

Match the virus with its corresponding property.
-Surface antigen

Accepted Answer

A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B 
A) RSV 
B) HIV 
C) CMV 
D) Adenovirus 
E) HSV
F)Pediatric respiratory virus
G)Hepatitis B virus (HBV)
H)Influenzas A and B

Exam 64: Overview of the Methods and Strategies in Virology

Which assay is the current recommended method of detection in most laboratories for influenzas A and B viruses?

Match the virus with its corresponding property. -HEp-2 culture cells

Match the virus with the appropriate specimen. -Vesicular

Some viruses have the ability to stimulate uncontrolled growth of the host cells, resulting in cancer.Which virus has oncogenic properties?

Match the virus with the appropriate specimen. -Cerebrospinal fluid (CSF)

The function of the glycoprotein spike on some viruses is to:

Match the virus with its corresponding property. -R-Mix cells

Match the virus with its corresponding property. -Hemadsorption

The specimen of choice to collect in suspected cases of respiratory syncytial virus (RSV)is:

The most used EIA for antigen detection that uses a solid phase and membrane is used for detecting which virus?

A 2-year-old girl is suspected of having a rotavirus infection.The specimen of choice to collect to identify the agent is:

Match the virus with the appropriate specimen. -Blood

The cytomegalovirus (CMV)produces cytopathic effects (CPE)in diploid fibroblast cells in an average of 7 days.The results are available within 16 hours using which type of assay?

Match the virus with its corresponding property. -Adenovirus serotypes 40 and 41

MATCHING Match the virus with the specimen in which it is most commonly isolated. -Genital

Match the virus with its corresponding property. -Surface antigen

Microbial Taxonomy

Bacterial Genetics, Metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Immunochemical Methods Used for Organism Detection

Serologic Diagnosis of Infectious Diseases

Principles of Antimicrobial Action and Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus Micrococcus and Similar Organisms

Streptococcus Enterococcus and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix Lactobacillus and Similar Organisms

Nocardia Streptomyces Rhodococcus and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, Aeromonas, Chromobacterium, and Related Organisms

Sphingomonas Paucimobilis and Similar Organisms

Moraxella and Related Organisms

Eikenella and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Aggregatibacter, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis, Bordetella Parapertussis, and Related Species

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Overview of Anaerobic Organisms

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Laboratory Methods for Diagnosis of Parasitic Infections: Overview

Intestinal Protozoa

Blood and Tissue Protozoa

Other Protozoa

Intestinal Nematodes Roundworms

Tissue Nematodes Roundworms

Blood and Tissue Filarialnematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Mucorales Zygomycetes, Dermatophytes, and Opportunitistic and Systemic Mycoses

Dematiaceous Melanizedmolds

Opportunistic Atypical Fungus: Pneumocystis Jiroveci

The Yeasts

Anti-fungal Susceptibility Testing, Therapy, and Prevention