Question 1

A highly useful method for determining the presence of hydrolyzed fragments of DNA separated by electrophoresis is

Accepted Answer

A)  autoradiography. 
B)  x-ray crystallography. 
C)  analytical ultracentrifugation. 
D)  all of the above 
A)  autoradiography. 
B)  x-ray crystallography. 
C)  analytical ultracentrifugation. 
D)  all of the above A

Question 2

One major obstacle to using bacteria to produce human proteins is because addition of sugar residues to the proteins,glycosylation,never occurs properly.

Accepted Answer

A) True 
 B)False  True

Question 3

When viruses are used as vectors in gene therapy

Accepted Answer

A)  they are not to transform cells taken from the patient's body to give back to the patient 
B)  they can cause unexpected difficulties 
C)  both of the above 
D)  neither of the above 
A)  they are not to transform cells taken from the patient's body to give back to the patient 
B)  they can cause unexpected difficulties 
C)  both of the above 
D)  neither of the above B

Question 4

A chance to be a forensic scientist.RFLP analysis has been requested by the prosecuting attorney in a murder case.DNA samples have been isolated from a blood sample at the crime scene (C),a hair follicle from the victim's hairbrush (V),and a mouth swab of the accused (A).The results of the test are shown.What would you tell the attorney?

Accepted Answer

A)  The results indicate the accused is guilty. 
B)  The results indicate the accused is innocent. 
C)  The results are inconclusive. 
A)  The results indicate the accused is guilty. 
B)  The results indicate the accused is innocent. 
C)  The results are inconclusive.

Question 5

A feature of the Sanger-Coulson method for DNA sequencing is

Accepted Answer

A)  chemical modification of all bases 
B)  no need to use gel electrophoresis 
C)  use of triple-helical DNA 
D)  direct "reading" of the sequence of bases 
A)  chemical modification of all bases 
B)  no need to use gel electrophoresis 
C)  use of triple-helical DNA 
D)  direct "reading" of the sequence of bases

Question 6

SyBr green and SyBr gold were developed because

Accepted Answer

A)  ethidium bromide is not sensitive enough for modern research 
B)  they allow faster electrophoretic separations of DNA 
C)  they fluoresce with RNA as well as DNA, while ethidium bromide does not 
D)  ethidium bromide is carcinogenic 
A)  ethidium bromide is not sensitive enough for modern research 
B)  they allow faster electrophoretic separations of DNA 
C)  they fluoresce with RNA as well as DNA, while ethidium bromide does not 
D)  ethidium bromide is carcinogenic

Question 7

Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs.The b-gal gene codes for the enzyme b-galactosidase,the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpR gene confers ampicillin resistance.
  Refer to Exhibit 13D.In three separate vessels,the plasmid is treated with the restriction endonucleases EcoRI,BamHI,and HindIII.Which of the following best represents the electrophoretic gel one would see from these digests?

Accepted Answer

A)    
B)    
C)    
D)    
A)    
B)    
C)    
D)

Question 8

Suitable vectors for cloning of recombinant DNA can be

Accepted Answer

A)  bacteriophages. 
B)  bacterial plasmids. 
C)  both of these. 
D)  neither of these. 
A)  bacteriophages. 
B)  bacterial plasmids. 
C)  both of these. 
D)  neither of these.

Question 9

Exhibit 13A This is a map of the pBR322 plasmid,numbers on the map refer to the base where the site is located.
Refer to Exhibit 13A.If a recombinant plasmid were obtained inserting DNA into the BamHI site,the recombinant plasmid would lack which of the following properties?

Accepted Answer

A)  It would replicate autonomously. 
B)  It would be resistant to the antibiotic tetracycline. 
C)  It would be resistant to the antibiotic ampicillin. 
D)  It would possess at least two promoter sites.   
A)  It would replicate autonomously. 
B)  It would be resistant to the antibiotic tetracycline. 
C)  It would be resistant to the antibiotic ampicillin. 
D)  It would possess at least two promoter sites.

Question 10

A DNA library consists of

Accepted Answer

A)  all the DNA of an organism divided up and cloned in suitable vectors 
B)  an alphabetical list of all the genes in an organism 
C)  a set of reference books on genetics 
D)  none of the above 
A)  all the DNA of an organism divided up and cloned in suitable vectors 
B)  an alphabetical list of all the genes in an organism 
C)  a set of reference books on genetics 
D)  none of the above

Question 11

Fluorescence and other luminescent methods of visualizing bands offer these advantages over methods dependent on radioactivity.

Accepted Answer

A)  They can detect smaller amounts of material (they are more sensitive). 
B)  Fluorescent molecules like ethidium bromide offer no health hazard, while radioisotopes do. 
C)  Special licenses are required to work with radioisotopes. 
D)  These methods are both more sensitive and require no special license. 
E)  All of these are advantages. 
A)  They can detect smaller amounts of material (they are more sensitive). 
B)  Fluorescent molecules like ethidium bromide offer no health hazard, while radioisotopes do. 
C)  Special licenses are required to work with radioisotopes. 
D)  These methods are both more sensitive and require no special license. 
E)  All of these are advantages.

Question 12

Ethidium bromide slips between the bases of DNA giving it fluorescent properties that differ from those observed when it is free in solution.

Accepted Answer

A) True 
 B)False

Question 13

Exhibit 13A This is a map of the pBR322 plasmid,numbers on the map refer to the base where the site is located.
  Refer to Exhibit 13A.If a recombinant plasmid were obtained by inserting DNA into the EcoRV site,and the protein corresponding to the recombinant gene were expressed,which of the following statements would be false?

Accepted Answer

A)  The protein will probably contain some extra amino acids on its N-terminal. 
B)  The plasmid will be resistant to ampicillin. 
C)  The plasmid will not be able to replicate autonomously. 
D)  The protein is not likely to be biologically active without some further treatment. 
E)  The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis. 
A)  The protein will probably contain some extra amino acids on its N-terminal. 
B)  The plasmid will be resistant to ampicillin. 
C)  The plasmid will not be able to replicate autonomously. 
D)  The protein is not likely to be biologically active without some further treatment. 
E)  The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis.

Question 14

Antibiotic-resistance genes in vectors are often used as selectable markers.

Accepted Answer

A) True 
 B)False

Question 15

A single clone of interest can be distinguished from others in a mixture of clones by

Accepted Answer

A)  testing the clones for antibiotic resistance. 
B)  mobility of the clones in gel electrophoresis. 
C)  a specific probe, usually a labeled complementary DNA. 
D)  resistance to damage by ultraviolet light. 
A)  testing the clones for antibiotic resistance. 
B)  mobility of the clones in gel electrophoresis. 
C)  a specific probe, usually a labeled complementary DNA. 
D)  resistance to damage by ultraviolet light.

Question 16

While a large majority of people are opposed to somatic gene therapy,a large number support the use of germ-cell gene therapy.

Accepted Answer

A) True 
 B)False

Question 17

The usefulness of blotting techniques in molecular biology is that

Accepted Answer

A)  spills of hazardous chemicals are minimized 
B)  only the substance of interest is transferred to a nitrocellulose disk 
C)  it directly gives rise to a genetic map 
D)  transferred material is in the same relative position on the disk as on the original sample 
A)  spills of hazardous chemicals are minimized 
B)  only the substance of interest is transferred to a nitrocellulose disk 
C)  it directly gives rise to a genetic map 
D)  transferred material is in the same relative position on the disk as on the original sample

Question 18

Which of the following is true?

Accepted Answer

A)  When DNA evidence is used in crime cases, it is easier to prove that a person is guilty than that they are innocent. 
B)  When DNA evidence is used in a paternity case, it is easier to prove that a person is the father rather than the person is not the fathere 
C)  DNA evidence is easier to use to exclude a putative match than to include it 
D)  none of these is true 
A)  When DNA evidence is used in crime cases, it is easier to prove that a person is guilty than that they are innocent. 
B)  When DNA evidence is used in a paternity case, it is easier to prove that a person is the father rather than the person is not the fathere 
C)  DNA evidence is easier to use to exclude a putative match than to include it 
D)  none of these is true

Question 19

The application of gel electrophoresis (PAGE) in DNA sequencing

Accepted Answer

A)  is nonexistent because DNA fragments differ widely in charge. 
B)  depends on separation of similar charge/mass ratio on the basis of size. 
C)  depends on separation of identical size on the basis of charge/mass ratio. 
D)  is nonexistent because DNA fragments have no net charge. 
A)  is nonexistent because DNA fragments differ widely in charge. 
B)  depends on separation of similar charge/mass ratio on the basis of size. 
C)  depends on separation of identical size on the basis of charge/mass ratio. 
D)  is nonexistent because DNA fragments have no net charge.

Question 20

Which of the following statements about plants which contain the recombinant form of the gene from Bacillus thuringiensis (Bt gene),is true?

Accepted Answer

A)  These plants have a natural resistance to caterpillars. 
B)  The gene is expressed in all the plant tissues. 
C)  Since the gene is expressed in the pollen of the plant, there is concern about the gene harming beneficial insects. 
D)  It is likely that some insects will mutate to become resistant to the Bt gene product. 
E)  All of these statements are true. 
A)  These plants have a natural resistance to caterpillars. 
B)  The gene is expressed in all the plant tissues. 
C)  Since the gene is expressed in the pollen of the plant, there is concern about the gene harming beneficial insects. 
D)  It is likely that some insects will mutate to become resistant to the Bt gene product. 
E)  All of these statements are true.

Exam 13: Nucleic Acid Biotechnology Techniques

A highly useful method for determining the presence of hydrolyzed fragments of DNA separated by electrophoresis is

One major obstacle to using bacteria to produce human proteins is because addition of sugar residues to the proteins,glycosylation,never occurs properly.

When viruses are used as vectors in gene therapy

A feature of the Sanger-Coulson method for DNA sequencing is

SyBr green and SyBr gold were developed because

Suitable vectors for cloning of recombinant DNA can be

Exhibit 13A This is a map of the pBR322 plasmid,numbers on the map refer to the base where the site is located. Refer to Exhibit 13A.If a recombinant plasmid were obtained inserting DNA into the BamHI site,the recombinant plasmid would lack which of the following properties?

A DNA library consists of

Fluorescence and other luminescent methods of visualizing bands offer these advantages over methods dependent on radioactivity.

Ethidium bromide slips between the bases of DNA giving it fluorescent properties that differ from those observed when it is free in solution.

Antibiotic-resistance genes in vectors are often used as selectable markers.

A single clone of interest can be distinguished from others in a mixture of clones by

While a large majority of people are opposed to somatic gene therapy,a large number support the use of germ-cell gene therapy.

The usefulness of blotting techniques in molecular biology is that

Which of the following is true?

The application of gel electrophoresis (PAGE) in DNA sequencing

Which of the following statements about plants which contain the recombinant form of the gene from Bacillus thuringiensis (Bt gene),is true?

Biochemistry and the Organization of Cells

Water: The Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Biosynthesis of Nucleic Acids: Replication

Transcription of the Genetic Code: Biosynthesis of Rna

Protein Synthesis: Translation of the Genetic Message

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

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