Question 1

Protein kinase A is converted from an inactive state to an active state by binding:

Accepted Answer

A) ATP. 
B) calcium. 
C) cAMP. 
D) ATP and cAMP. 
A) ATP. 
B) calcium. 
C) cAMP. 
D) ATP and cAMP.

Question 2

Gel filtration chromatography separates proteins on the basis of their:

Accepted Answer

A) charge. 
B) mass. 
C) affinity for a ligand. 
D) mass and charge. 
A) charge. 
B) mass. 
C) affinity for a ligand. 
D) mass and charge.

Question 3

Which of the following is a mechanism for regulating protein activity?

Accepted Answer

A) proteolytic processing 
B) phosphorylation/dephosphorylation 
C) ligand binding 
D) all of the above 
A) proteolytic processing 
B) phosphorylation/dephosphorylation 
C) ligand binding 
D) all of the above

Question 4

When comparing domains and structural motifs,which of the following is NOT true?

Accepted Answer

A) Motifs are found in secondary structures,while domains are found in tertiary structures. 
B) Helices are observed in motifs and domains. 
C) Structural domains appear in different proteins with similar functions,while structural motifs have been less conserved over evolution. 
D) A domain may be repeated in the same protein,but multiple copies of the same motif are rare. 
A) Motifs are found in secondary structures,while domains are found in tertiary structures. 
B) Helices are observed in motifs and domains. 
C) Structural domains appear in different proteins with similar functions,while structural motifs have been less conserved over evolution. 
D) A domain may be repeated in the same protein,but multiple copies of the same motif are rare.

Question 5

Kinases,which are responsible for the activation or inactivation of a number of proteins,add phosphate groups onto:

Accepted Answer

A) tryptophan residues. 
B) serine residues. 
C) cysteine residues. 
D) tryptophan and cysteine residues. 
A) tryptophan residues. 
B) serine residues. 
C) cysteine residues. 
D) tryptophan and cysteine residues.

Question 6

Medical researchers are developing new clinical tests that detect and analyze the expression of multiple proteins and protein complexes in the hope that they might improve diagnosis of diseases such as early stage cancers.What techniques might researchers use in these studies?

Accepted Answer

They might use protein separation techni

Question 7

Which of the following plays a role in the degradation of proteins?

Accepted Answer

A) RNAi 
B) ubiquitin 
C) proteasome 
D) b and c 
A) RNAi 
B) ubiquitin 
C) proteasome 
D) b and c

Question 8

What is Western blotting? How can this technique be used to detect proteins?

Accepted Answer

Western blotting or immunoblotting is a

Question 9

Which of the following methods can separate proteins based on their mass?

Accepted Answer

A) centrifugation 
B) ion exchange chromatography 
C) SDS polyacrylamide gel electrophoresis 
D) centrifugation and SDS polyacrylamide gel electrophoresis 
A) centrifugation 
B) ion exchange chromatography 
C) SDS polyacrylamide gel electrophoresis 
D) centrifugation and SDS polyacrylamide gel electrophoresis

Question 10

Starting with 1 mCi (milliCurie)of a phosphorus-32-labeled compound,how long would it take until only 0.125 mCi remains?

Accepted Answer

A) 14.3 days 
B) 28.6 days 
C) 42.9 days 
D) 57.2 days 
A) 14.3 days 
B) 28.6 days 
C) 42.9 days 
D) 57.2 days

Question 11

Which of the following is NOT part of a zinc-finger motif?

Accepted Answer

A) zinc ion 
B) proline residue 
C) cysteine residue 
D) histidine residue 
A) zinc ion 
B) proline residue 
C) cysteine residue 
D) histidine residue

Question 12

For an enzyme-catalyzed reaction,doubling the concentration of enzyme will:

Accepted Answer

A) double the Vmₐₓ. 
B) halve the Vmₐₓ. 
C) double the Km. 
D) halve the Km. 
A) double the Vmₐₓ. 
B) halve the Vmₐₓ. 
C) double the Km. 
D) halve the Km.

Question 13

Proteomics allows researchers to:

Accepted Answer

A) examine where a protein is located within the cytosol of a cell. 
B) compare thousands of samples from different people in the same experiment. 
C) examine which proteins differ in abundance between a normal sample and a disease sample. 
D) use antibodies to label specific proteins on an SDS-PAGE gel. 
A) examine where a protein is located within the cytosol of a cell. 
B) compare thousands of samples from different people in the same experiment. 
C) examine which proteins differ in abundance between a normal sample and a disease sample. 
D) use antibodies to label specific proteins on an SDS-PAGE gel.

Question 14

Which of the following is defined as the tertiary structure of a protein?

Accepted Answer

A) the primary amino acid sequence 
B) structural domains such as a DNA-binding domain 
C) folded structures such as an α helix 
D) structural features such as a turn 
A) the primary amino acid sequence 
B) structural domains such as a DNA-binding domain 
C) folded structures such as an α helix 
D) structural features such as a turn

Question 15

A misfolded protein targeted to the proteasome will undergo:

Accepted Answer

A) unfolding using energy released by ATPases. 
B) entry into the proteasome through the narrow channel in the beta channel. 
C) death by a thousand cuts in the alpha subunit rings. 
D) complete cleavage into its amino acid monomers. 
A) unfolding using energy released by ATPases. 
B) entry into the proteasome through the narrow channel in the beta channel. 
C) death by a thousand cuts in the alpha subunit rings. 
D) complete cleavage into its amino acid monomers.

Question 16

Describe the mechanism by which the bacterial chaperonin GroEL promotes protein folding.

Accepted Answer

The bacterial chaperonin GroEL forms a b

Question 17

Many proteins contain one or more motifs built from particular combinations of secondary structure.Describe the three common structural motifs discussed in this chapter.

Accepted Answer

The three structural motifs described in

Question 18

A protein containing several proline residues is:

Accepted Answer

A) not likely to form quarternary structures. 
B) likely to be an integral membrane protein. 
C) not likely to form alpha helices. 
D) likely to be found in beta turns. 
A) not likely to form quarternary structures. 
B) likely to be an integral membrane protein. 
C) not likely to form alpha helices. 
D) likely to be found in beta turns.

Question 19

Monomeric proteins do not contain a:

Accepted Answer

A) primary structure. 
B) secondary structure. 
C) tertiary structure. 
D) quaternary structure. 
A) primary structure. 
B) secondary structure. 
C) tertiary structure. 
D) quaternary structure.

Question 20

A chunk of tissue is treated so that each cell's membrane is broken open to release the contents inside,and then subjected to differential centrifugation.Which of the following is true at the end of the centrifugation?

Accepted Answer

A) Depending on the speed of the centrifugation,the proteasome is more likely to be in the supernatant than a chaperone. 
B) Proteins of similar density will be found in the same fraction (either pellet or supernatant). 
C) The pellet contains the least dense material. 
D) The pellet will contain a purified protein for further analysis. 
A) Depending on the speed of the centrifugation,the proteasome is more likely to be in the supernatant than a chaperone. 
B) Proteins of similar density will be found in the same fraction (either pellet or supernatant). 
C) The pellet contains the least dense material. 
D) The pellet will contain a purified protein for further analysis.

Exam 3: Protein Structure and Function

Protein kinase A is converted from an inactive state to an active state by binding:

Gel filtration chromatography separates proteins on the basis of their:

Which of the following is a mechanism for regulating protein activity?

When comparing domains and structural motifs,which of the following is NOT true?

Kinases,which are responsible for the activation or inactivation of a number of proteins,add phosphate groups onto:

Medical researchers are developing new clinical tests that detect and analyze the expression of multiple proteins and protein complexes in the hope that they might improve diagnosis of diseases such as early stage cancers.What techniques might researchers use in these studies?

Which of the following plays a role in the degradation of proteins?

What is Western blotting? How can this technique be used to detect proteins?

Which of the following methods can separate proteins based on their mass?

Starting with 1 mCi (milliCurie)of a phosphorus-32-labeled compound,how long would it take until only 0.125 mCi remains?

Which of the following is NOT part of a zinc-finger motif?

For an enzyme-catalyzed reaction,doubling the concentration of enzyme will:

Proteomics allows researchers to:

Which of the following is defined as the tertiary structure of a protein?

A misfolded protein targeted to the proteasome will undergo:

Describe the mechanism by which the bacterial chaperonin GroEL promotes protein folding.

Many proteins contain one or more motifs built from particular combinations of secondary structure.Describe the three common structural motifs discussed in this chapter.

A protein containing several proline residues is:

Monomeric proteins do not contain a:

A chunk of tissue is treated so that each cell's membrane is broken open to release the contents inside,and then subjected to differential centrifugation.Which of the following is true at the end of the centrifugation?

Chemical Foundations

Molecular Genetic Techniques

Culturing and Visualizing Cells

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Transcriptional Control of Gene Expression

Post-Transcriptional Gene Control

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

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