Question 1

Which of the following allows one to circumvent the theoretical resolution of the microscope?

Accepted Answer

A) total internal reflection fluorescence microcopy 
B) photo-activated localization microscopy 
C) indirect immunofluorescence microscopy 
D) double-label fluorescence microscopy 
A) total internal reflection fluorescence microcopy 
B) photo-activated localization microscopy 
C) indirect immunofluorescence microscopy 
D) double-label fluorescence microscopy

Question 2

Fluorescence microscopy of intact organisms or large cells results in the generation of blurred images.Which of the following is NOT a technique that reduces the out-of-focus signal that causes blurring?

Accepted Answer

A) two-photon excitation microscopy 
B) confocal microscopy 
C) fluorescence recovery after photobleaching (FRAP) 
D) deconvolution microscopy 
A) two-photon excitation microscopy 
B) confocal microscopy 
C) fluorescence recovery after photobleaching (FRAP) 
D) deconvolution microscopy

Question 3

Ultracentrifuges allow cell biologists to isolate mitochondria from lysosomes based on organelle differences in:

Accepted Answer

A) isoelectric point. 
B) ionic composition. 
C) equilibrium density. 
D) size. 
A) isoelectric point. 
B) ionic composition. 
C) equilibrium density. 
D) size.

Question 4

Primary cultures contain _____ (this cell type)which quickly predominate over the other cell types.Given the proper space and nutrients needed to grow,these cells can divide about 50 times,such that after 50 doublings,an original culture of 100 cells would become _____ cells.

Accepted Answer

A) fibroblasts;2500 
B) fibroblasts;1.1 × 10¹⁷ 
C) epithelial cells;2500 
D) epithelial cells;4.5 × 10¹⁵ 
A) fibroblasts;2500 
B) fibroblasts;1.1 × 10¹⁷ 
C) epithelial cells;2500 
D) epithelial cells;4.5 × 10¹⁵

Question 5

To visualize cells by immunofluorescence microscopy,the cells must be:

Accepted Answer

A) placed in a vacuum. 
B) living. 
C) sectioned. 
D) permeabilized. 
A) placed in a vacuum. 
B) living. 
C) sectioned. 
D) permeabilized.

Question 6

Fixatives such as formaldehyde are routinely used in certain types of electron microscopy and light microscopy.However,fixatives may introduce complications in the analysis of the resulting images.What problems may result from using fixatives?

Accepted Answer

Most fixatives are cross-linking agents

Question 7

All of the following are produced by animal cells in culture and help the cells adhere to the culture dish except:

Accepted Answer

A) glycoproteins. 
B) collagen. 
C) phospholipase A. 
D) hyaluronic acid. 
A) glycoproteins. 
B) collagen. 
C) phospholipase A. 
D) hyaluronic acid.

Question 8

The fluorescent properties of dyes such as SNARF-1 can provide information on the:

Accepted Answer

A) location of specific proteins. 
B) concentration of H⁺ ions in specific regions of the cell. 
C) the amount of RNA in a cell. 
D) volume of a cell. 
A) location of specific proteins. 
B) concentration of H⁺ ions in specific regions of the cell. 
C) the amount of RNA in a cell. 
D) volume of a cell.

Question 9

Rough endoplasmic reticulum can be separated from smooth endoplasmic reticulum by differential centrifugation.What is the basis for this fractionation?

Accepted Answer

During cell disruption,the endoplasmic r

Question 10

Drugs used to inhibit which molecules would reduce the amount of antibody produced by hybridomas?

Accepted Answer

A) proteasome inhibitors 
B) protease inhibitors 
C) transcription inhibitors 
D) antibiotics 
A) proteasome inhibitors 
B) protease inhibitors 
C) transcription inhibitors 
D) antibiotics

Question 11

The enzymatic functions of specific organelles can best be examined:

Accepted Answer

A) in intact cells where the organelles are working as they normally would. 
B) in cells that have been prepared for SEM (scanning electron microscopy). 
C) in fractions taken from cell homogenates that have been separated by differential centrifugation. 
D) in fractions taken from differential centrifugation of cellular homogenates that are further incubated with antibodies specific to the organelle under study,which can be used to precipitate the organelles away from contaminates. 
A) in intact cells where the organelles are working as they normally would. 
B) in cells that have been prepared for SEM (scanning electron microscopy). 
C) in fractions taken from cell homogenates that have been separated by differential centrifugation. 
D) in fractions taken from differential centrifugation of cellular homogenates that are further incubated with antibodies specific to the organelle under study,which can be used to precipitate the organelles away from contaminates.

Question 12

What factors necessary for growth of animal cells in culture are provided by serum?

Accepted Answer

A) amino acids 
B) precursors of DNA synthesis 
C) growth factors 
D) vitamins 
A) amino acids 
B) precursors of DNA synthesis 
C) growth factors 
D) vitamins

Question 13

Why are cells cultured in the lab such a useful model system for studying human disease?

Accepted Answer

A) Cells only function when they are not organized into tissues. 
B) Humans are a varied population and extrapolating from physiological studies yields mixed results. 
C) Cells cultured in the lab behave exactly the same as cells in the human body. 
D) Cells are very inexpensive to grow. 
A) Cells only function when they are not organized into tissues. 
B) Humans are a varied population and extrapolating from physiological studies yields mixed results. 
C) Cells cultured in the lab behave exactly the same as cells in the human body. 
D) Cells are very inexpensive to grow.

Question 14

Characteristics of transformed cells can include all of the following EXCEPT:

Accepted Answer

A) aneuploidy. 
B) ability to differentiate into different cell types. 
C) tight junctions. 
D) presence of integrated viral genes. 
A) aneuploidy. 
B) ability to differentiate into different cell types. 
C) tight junctions. 
D) presence of integrated viral genes.

Question 15

In your cell biology laboratory class,you are given a sample of blood.Which of the following results are NOT possible from your analysis of this blood using flow cytometry?

Accepted Answer

A) isolation of the largest and most dense cells from the smaller blood cells 
B) measurement of the amount of DNA in white blood cells 
C) relative quantitation of the sizes and shapes of the cells in the blood sample 
D) after the addition of fluorescent antibodies specific to T cells,the analysis of T cell abundance and size relative to other cells in the sample 
A) isolation of the largest and most dense cells from the smaller blood cells 
B) measurement of the amount of DNA in white blood cells 
C) relative quantitation of the sizes and shapes of the cells in the blood sample 
D) after the addition of fluorescent antibodies specific to T cells,the analysis of T cell abundance and size relative to other cells in the sample

Question 16

The best 3-D images of cellular organelles come from:

Accepted Answer

A) cryoelectron microscopy. 
B) immunoelectron microscopy. 
C) thin-section electron microscopy. 
D) low-angle rotary shadowing with TEM. 
A) cryoelectron microscopy. 
B) immunoelectron microscopy. 
C) thin-section electron microscopy. 
D) low-angle rotary shadowing with TEM.

Which of the following allows one to circumvent the theoretical resolution of the microscope?

Fluorescence microscopy of intact organisms or large cells results in the generation of blurred images.Which of the following is NOT a technique that reduces the out-of-focus signal that causes blurring?

Ultracentrifuges allow cell biologists to isolate mitochondria from lysosomes based on organelle differences in:

Primary cultures contain _ (this cell type)which quickly predominate over the other cell types.Given the proper space and nutrients needed to grow,these cells can divide about 50 times,such that after 50 doublings,an original culture of 100 cells would become _ cells.

To visualize cells by immunofluorescence microscopy,the cells must be:

Fixatives such as formaldehyde are routinely used in certain types of electron microscopy and light microscopy.However,fixatives may introduce complications in the analysis of the resulting images.What problems may result from using fixatives?

All of the following are produced by animal cells in culture and help the cells adhere to the culture dish except:

The fluorescent properties of dyes such as SNARF-1 can provide information on the:

Rough endoplasmic reticulum can be separated from smooth endoplasmic reticulum by differential centrifugation.What is the basis for this fractionation?

Drugs used to inhibit which molecules would reduce the amount of antibody produced by hybridomas?

The enzymatic functions of specific organelles can best be examined:

What factors necessary for growth of animal cells in culture are provided by serum?

Why are cells cultured in the lab such a useful model system for studying human disease?

Characteristics of transformed cells can include all of the following EXCEPT:

In your cell biology laboratory class,you are given a sample of blood.Which of the following results are NOT possible from your analysis of this blood using flow cytometry?

The best 3-D images of cellular organelles come from:

Chemical Foundations

Molecular Genetic Techniques

Protein Structure and Function

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Transcriptional Control of Gene Expression

Post-Transcriptional Gene Control

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

Filters

Exam 4: Culturing and Visualizing Cells

Which of the following allows one to circumvent the theoretical resolution of the microscope?

Fluorescence microscopy of intact organisms or large cells results in the generation of blurred images.Which of the following is NOT a technique that reduces the out-of-focus signal that causes blurring?

Ultracentrifuges allow cell biologists to isolate mitochondria from lysosomes based on organelle differences in:

Primary cultures contain _____ (this cell type)which quickly predominate over the other cell types.Given the proper space and nutrients needed to grow,these cells can divide about 50 times,such that after 50 doublings,an original culture of 100 cells would become _____ cells.

To visualize cells by immunofluorescence microscopy,the cells must be:

Fixatives such as formaldehyde are routinely used in certain types of electron microscopy and light microscopy.However,fixatives may introduce complications in the analysis of the resulting images.What problems may result from using fixatives?

All of the following are produced by animal cells in culture and help the cells adhere to the culture dish except:

The fluorescent properties of dyes such as SNARF-1 can provide information on the:

Rough endoplasmic reticulum can be separated from smooth endoplasmic reticulum by differential centrifugation.What is the basis for this fractionation?

Drugs used to inhibit which molecules would reduce the amount of antibody produced by hybridomas?

The enzymatic functions of specific organelles can best be examined:

What factors necessary for growth of animal cells in culture are provided by serum?

Why are cells cultured in the lab such a useful model system for studying human disease?

Characteristics of transformed cells can include all of the following EXCEPT:

In your cell biology laboratory class,you are given a sample of blood.Which of the following results are NOT possible from your analysis of this blood using flow cytometry?

The best 3-D images of cellular organelles come from:

Chemical Foundations

Molecular Genetic Techniques

Protein Structure and Function

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Transcriptional Control of Gene Expression

Post-Transcriptional Gene Control

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

Filters

Primary cultures contain _ (this cell type)which quickly predominate over the other cell types.Given the proper space and nutrients needed to grow,these cells can divide about 50 times,such that after 50 doublings,an original culture of 100 cells would become _ cells.