Question 1

Gene probes can be labeled for detection with

Accepted Answer

A) enzymes. 
B) fluorescent dyes. 
C) radioisotopes. 
D) All of the choices are correct. 
E) None of the choices are correct. 
A) enzymes. 
B) fluorescent dyes. 
C) radioisotopes. 
D) All of the choices are correct. 
E) None of the choices are correct. D

Question 2

Transgenic animals are _____ modified organisms.

Accepted Answer

genetically

Question 3

If you have the sequence of mRNA that is AUAUGC, then the DNA antisense strand is ____.

Accepted Answer

A) AUAUGC 
B) UAUACG 
C) TATACG 
D) CGUAUA 
A) AUAUGC 
B) UAUACG 
C) TATACG 
D) CGUAUA C

Question 4

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Accepted Answer

A) PCR 
B) genetic engineering 
C) antisense technology 
D) microarray analysis 
E) DNA sequencing 
A) PCR 
B) genetic engineering 
C) antisense technology 
D) microarray analysis 
E) DNA sequencing

Question 5

The fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A) DNA. 
B) RNA. 
C) proteins. 
D) recombinant DNA. 
E) specific genetic marker sequence on genes. 
A) DNA. 
B) RNA. 
C) proteins. 
D) recombinant DNA. 
E) specific genetic marker sequence on genes.

Question 6

Two different nucleic acids can _____ by uniting at their complementary sites.

Accepted Answer

The answer of Two different nucleic acids can _____ by...

Question 7

The enzyme___ adds three nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated

Accepted Answer

A) DNA polymerase III, 3'$\rarr$5' 
B) DNA polymerase I, 3'$\rarr$5' 
C) DNA polymerase III, 5'$\rarr$3' 
D) DNA polymerase I, 5'$\rarr$3' 
E) None of the above 
A) DNA polymerase III, 3'$\rarr$5' 
B) DNA polymerase I, 3'$\rarr$5' 
C) DNA polymerase III, 5'$\rarr$3' 
D) DNA polymerase I, 5'$\rarr$3' 
E) None of the above

Question 8

The polymerase chain reaction (PCR) has been used in all of the following fields, except

Accepted Answer

A) forensics. 
B) evolutionary studies. 
C) gene mapping. 
D) medicine. 
E) None of the choices; it is useful for all these fields. 
A) forensics. 
B) evolutionary studies. 
C) gene mapping. 
D) medicine. 
E) None of the choices; it is useful for all these fields.

Question 9

The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA sequencing is to

Accepted Answer

A) denature DNA into single strands. 
B) serve as primers. 
C) be a fluorescent tag. 
D) incorporate into newly replicated DNA strands and stop elongation. 
E) None of the choices are correct. 
A) denature DNA into single strands. 
B) serve as primers. 
C) be a fluorescent tag. 
D) incorporate into newly replicated DNA strands and stop elongation. 
E) None of the choices are correct.

Question 10

DNA strands can be clipped crosswise at selected positions by using enzymes called

Accepted Answer

A) palindromes. 
B) reverse transcriptases. 
C) restriction endonucleases. 
D) ligases. 
E) DNA polymerases. 
A) palindromes. 
B) reverse transcriptases. 
C) restriction endonucleases. 
D) ligases. 
E) DNA polymerases.

Question 11

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Accepted Answer

A) human growth hormone 
B) Factor VIII 
C) insulin 
D) tissue plasminogen activator 
E) All of the choices are correct. 
A) human growth hormone 
B) Factor VIII 
C) insulin 
D) tissue plasminogen activator 
E) All of the choices are correct.

Question 13

All of the following are correct about DNA fingerprinting, except

Accepted Answer

A) it displays the unique pattern of an individual's DNA fragments. 
B) it can be used to analyze evidence when only minute amounts of DNA are found. 
C) it cannot be used on specimens older than five years. 
D) it is routine to use it as trial evidence in the U.S. 
E) it can be used on hair and saliva. 
A) it displays the unique pattern of an individual's DNA fragments. 
B) it can be used to analyze evidence when only minute amounts of DNA are found. 
C) it cannot be used on specimens older than five years. 
D) it is routine to use it as trial evidence in the U.S. 
E) it can be used on hair and saliva.

Question 14

Sequencing a gene automatically identifies which gene it is.

Accepted Answer

A) True 
 B)False

Question 15

When DNA is heated, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 16

When DNA is heated to just below boiling (90$\circ$C - 95$\circ$C),

Accepted Answer

A) the sugar phosphate backbone of the DNA strands breaks. 
B) the sugar, phosphate and base come apart in each nucleotide. 
C) the helix unwinds but hydrogen bonds between the bases remain intact. 
D) the two DNA strands separate completely. 
E) Nothing happens until the boiling point is reached (100$\circ$
C) . 
A) the sugar phosphate backbone of the DNA strands breaks. 
B) the sugar, phosphate and base come apart in each nucleotide. 
C) the helix unwinds but hydrogen bonds between the bases remain intact. 
D) the two DNA strands separate completely. 
E) Nothing happens until the boiling point is reached (100$\circ$
C) .

Question 17

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 18

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 19

EcoRI and HindIII are

Accepted Answer

A) palindromes. 
B) reverse transcriptases. 
C) restriction endonucleases. 
D) ligases. 
E) DNA polymerases. 
A) palindromes. 
B) reverse transcriptases. 
C) restriction endonucleases. 
D) ligases. 
E) DNA polymerases.

Question 20

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

Accepted Answer

1. Start by growing E. coli cells in a l

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Gene probes can be labeled for detection with

Transgenic animals are _____ modified organisms.

If you have the sequence of mRNA that is AUAUGC, then the DNA antisense strand is ____.

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

The fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Two different nucleic acids can _____ by uniting at their complementary sites.

The enzyme___ adds three nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated

The polymerase chain reaction (PCR) has been used in all of the following fields, except

The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA sequencing is to

DNA strands can be clipped crosswise at selected positions by using enzymes called

Restriction endonucleases are obtained from various species of bacteria.

Which of the following drugs is/are produced by genetic engineering and approved for human use?

All of the following are correct about DNA fingerprinting, except

Sequencing a gene automatically identifies which gene it is.

When DNA is heated, the two strands will separate.

When DNA is heated to just below boiling (90 ∘\circ∘ C - 95 ∘\circ∘ C),

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Reverse transcriptase is used to make cDNA from an RNA template.

EcoRI and HindIII are

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

An Introduction to Microbial Genetics

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host-The Elements of Chemotherapy

Microbe-Human Interactions: Infection, Disease, and Epidemiology

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

Filters

The enzyme_ adds three nucleotides of DNA in the _direction when DNA is being semi-conservatively replicated

When DNA is heated to just below boiling (90^$\circ$C - 95^$\circ$C),