Question 1

Which of the following is not a biochemical test used to identify a pathogen?

Accepted Answer

A) Acid-fast reaction 
B) Hydrolysis of gelatin 
C) Coagulase production 
D) Oxygen requirements 
E) Carbohydrate fermentation 
A) Acid-fast reaction 
B) Hydrolysis of gelatin 
C) Coagulase production 
D) Oxygen requirements 
E) Carbohydrate fermentation A

Question 2

Which of the following is involved in the in vitro diagnostic testing of serum?

Accepted Answer

A) mycology 
B) hematology 
C) serology 
D) histology 
E) virology 
A) mycology 
B) hematology 
C) serology 
D) histology 
E) virology C

Question 3

Which of the following choices are true about the dyes fluorescein and rhodamine?

Accepted Answer

A) They can be used to label antibodies in immunofluorescence tests. 
B) They emit visible light in response to ultraviolet radiation. 
C) They are observed in the fluorescent microscope. 
D) They re used to identify pathogens of chlamydiosis, Legionnaires' disease, and others. 
E) All of the choices are true. 
A) They can be used to label antibodies in immunofluorescence tests. 
B) They emit visible light in response to ultraviolet radiation. 
C) They are observed in the fluorescent microscope. 
D) They re used to identify pathogens of chlamydiosis, Legionnaires' disease, and others. 
E) All of the choices are true. E

Question 4

All of the following are methods to diagnose viral infections, except:

Accepted Answer

A) detection of viral nucleic acid using specific probes. 
B) Western blot. 
C) cells taken from patient are examined for evidence of viral infection. 
D) signs and symptoms. 
E) the light microscope. 
A) detection of viral nucleic acid using specific probes. 
B) Western blot. 
C) cells taken from patient are examined for evidence of viral infection. 
D) signs and symptoms. 
E) the light microscope.

Question 5

All of the following are correct about the indirect ELISA, except:

Accepted Answer

A) it can detect antibodies in a serum sample. 
B) it is the common screening test for antibodies to Helicobacter. 
C) color development indicates that antibody was not present in the patient's serum. 
D) it is the common screening test for antibodies to Hepatitis A. 
E) a known antigen is adsorbed to the surface of a well. 
A) it can detect antibodies in a serum sample. 
B) it is the common screening test for antibodies to Helicobacter. 
C) color development indicates that antibody was not present in the patient's serum. 
D) it is the common screening test for antibodies to Hepatitis A. 
E) a known antigen is adsorbed to the surface of a well.

Question 6

Whole antigens are detected in which type of test?

Accepted Answer

A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity 
A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity

Question 7

Precipitation tests involve all of the following, except:

Accepted Answer

A) they rely on formation of visible clumps for detection. 
B) they include the VDRL test for syphilis. 
C) they are often performed in agar gels. 
D) they can be done in a test tube by carefully adding antiserum over antigen solution. 
E) a cloudy or opaque zone developing where antigen and antibody react 
A) they rely on formation of visible clumps for detection. 
B) they include the VDRL test for syphilis. 
C) they are often performed in agar gels. 
D) they can be done in a test tube by carefully adding antiserum over antigen solution. 
E) a cloudy or opaque zone developing where antigen and antibody react

Question 8

Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?

Accepted Answer

A) Ouchterlony double diffusion 
B) Western blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test 
A) Ouchterlony double diffusion 
B) Western blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test

Question 9

The property of a test to detect only a certain antibody or antigen, and not to react with any others, is:

Accepted Answer

A) cross-reactions. 
B) agglutination. 
C) precipitation. 
D) specificity. 
E) sensitivity. 
A) cross-reactions. 
B) agglutination. 
C) precipitation. 
D) specificity. 
E) sensitivity.

Question 10

Compare and contrast agglutination and precipitation reactions, and include a description of one test for each type of reaction.

Accepted Answer

Agglutination and precipitation reaction

Question 11

The physician orders an antistreptolysin O (ASO) titer test to check for rheumatic fever. A patient serum sample is exposed to known suspensions of streptolysin and then allowed to incubate with RBCs? If the patient does have rheumatic fever, what will happen?

Accepted Answer

A) Not enough information is provided to speculate. 
B) The red blood cells will lyse. 
C) The red blood cells will agglutinate only. 
D) The red blood cells will not lyse. 
E) None of the above.
F)The red blood cells will fluoresce. 
A) Not enough information is provided to speculate. 
B) The red blood cells will lyse. 
C) The red blood cells will agglutinate only. 
D) The red blood cells will not lyse. 
E) None of the above.
F)The red blood cells will fluoresce.

Question 12

A serum titer involves:

Accepted Answer

A) serially diluting a serum sample. 
B) determining the lowest dilution of serum that produces a visible reaction. 
C) determining the highest dilution of antigen that produces a visible reaction. 
D) the Western blot method. 
E) None of the above mentioned choices. 
A) serially diluting a serum sample. 
B) determining the lowest dilution of serum that produces a visible reaction. 
C) determining the highest dilution of antigen that produces a visible reaction. 
D) the Western blot method. 
E) None of the above mentioned choices.

Question 13

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Accepted Answer

A) Ouchterlony double diffusion 
B) Western blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test 
A) Ouchterlony double diffusion 
B) Western blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test

Question 14

In indirect fluorescent testing, fluorescent _____ are used to react with the Fc region of another, specific, serum antibody.

Accepted Answer

The answer of In indirect fluorescent testing, fluorescent _____ are...

Question 15

A rising antibody titer a few days apart indicates:

Accepted Answer

A) The patient had a past case of the disease. 
B) The patient has a current infection. 
C) Nothing, people always have antibodies in their serum. 
D) All of the above mentioned choices are possible. 
E) None of the above mentioned choices are possible. 
A) The patient had a past case of the disease. 
B) The patient has a current infection. 
C) Nothing, people always have antibodies in their serum. 
D) All of the above mentioned choices are possible. 
E) None of the above mentioned choices are possible.

Question 16

Horseradish peroxidase and alkaline phosphatase are enzymes used in _____ tests.

Accepted Answer

The answer of Horseradish peroxidase and alkaline phosphatase are enzymes...

Question 17

All of the following are routine biochemical tests to aid in identifying a species, except:

Accepted Answer

A) motility. 
B) carbohydrate fermentation. 
C) catalase test. 
D) oxidase test. 
E) coagulase test. 
A) motility. 
B) carbohydrate fermentation. 
C) catalase test. 
D) oxidase test. 
E) coagulase test.

Question 18

When antigen-antibody reactions are used to read a _____, they determine the quantity of antibodies in the serum.

Accepted Answer

The answer of When antigen-antibody reactions are used to read...

Question 19

The tuberculin test is an example of an in vivo serological test.

Accepted Answer

A) True 
 B)False

Question 20

Test systems involving which of the following has greatly improved the specificity of immunological tests?

Accepted Answer

A) monoclonal antibodies 
B) electronics 
C) enzymes 
D) radioactivity 
E) All of the choices are correct. 
A) monoclonal antibodies 
B) electronics 
C) enzymes 
D) radioactivity 
E) All of the choices are correct.

Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

Which of the following is not a biochemical test used to identify a pathogen?

Which of the following is involved in the in vitro diagnostic testing of serum?

Which of the following choices are true about the dyes fluorescein and rhodamine?

All of the following are methods to diagnose viral infections, except:

All of the following are correct about the indirect ELISA, except:

Whole antigens are detected in which type of test?

Precipitation tests involve all of the following, except:

Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?

The property of a test to detect only a certain antibody or antigen, and not to react with any others, is:

Compare and contrast agglutination and precipitation reactions, and include a description of one test for each type of reaction.

The physician orders an antistreptolysin O (ASO) titer test to check for rheumatic fever. A patient serum sample is exposed to known suspensions of streptolysin and then allowed to incubate with RBCs? If the patient does have rheumatic fever, what will happen?

A serum titer involves:

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

In indirect fluorescent testing, fluorescent _____ are used to react with the Fc region of another, specific, serum antibody.

A rising antibody titer a few days apart indicates:

Horseradish peroxidase and alkaline phosphatase are enzymes used in _____ tests.

All of the following are routine biochemical tests to aid in identifying a species, except:

When antigen-antibody reactions are used to read a _____, they determine the quantity of antibodies in the serum.

The tuberculin test is an example of an in vivo serological test.

Test systems involving which of the following has greatly improved the specificity of immunological tests?

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

An Introduction to Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host-The Elements of Chemotherapy

Microbe-Human Interactions: Infection, Disease, and Epidemiology

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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