Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

Sally had a throat sample taken at a satellite lab within her health care provider's office.What kind of media would be best suited for this specimen?

The type of microscope in which you would see brightly illuminated specimens against a black background is

Identification relies entirely on biochemical test results.

Compare and contrast the reagents and functions of negative staining versus positive staining.

One colony typically develops from the growth of several parent bacterial cells.

Media that contains extracts from plants, animals, or yeasts are

A contaminated food sample contains several different species of bacteria.A food microbiologist is interested in studying just one of these species.Describe the sequence of procedures that the microbiologist must perform in order to obtain a pure culture of the bacterial species of interest from this food sample.Detail all the necessary media and equipment.

In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?

Bacteria that require special growth factors and complex nutrients are termed _____.

The Gram stain, acid-fast stain, and endospore stain ____________.

_____ is the term for a culture made from one isolated colony.

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except

The Six I's of studying microorganisms include all of the following except

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

All of the following pertain to the fluorescence microscope except it

Mixed cultures are also referred to as contaminated cultures.

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Mannitol salt agar is selective for which bacterial genus?

Why is immersion oil often used when viewing specimens under the microscope?

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?