Question 1

An example of gene therapy is the insertion of the gene for human growth hormone into
E.coli cells.

Accepted Answer

A) True 
 B)False  False

Question 2

A technique that separates a readable pattern of DNA fragments is

Accepted Answer

A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA. 
D) gel electrophoresis. 
E) gene probes. 
A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA. 
D) gel electrophoresis. 
E) gene probes. D

Question 3

The primers in PCR are

Accepted Answer

A) synthetic DNA oligonucleotides. 
B) bacterial enzymes. 
C) short RNA strands. 
D) DNA polymerases. 
E) reverse transcriptases. 
A) synthetic DNA oligonucleotides. 
B) bacterial enzymes. 
C) short RNA strands. 
D) DNA polymerases. 
E) reverse transcriptases. A

Question 4

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Accepted Answer

A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA technology. 
D) gel electrophoresis. 
E) gene probes. 
A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA technology. 
D) gel electrophoresis. 
E) gene probes.

Question 5

Transformation and transfection are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 6

The Western Blot technique detects

Accepted Answer

A) DNA. 
B) RNA. 
C) proteins. 
D) recombinant DNA. 
E) specific genetic marker sequence on genes. 
A) DNA. 
B) RNA. 
C) proteins. 
D) recombinant DNA. 
E) specific genetic marker sequence on genes.

Question 7

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Accepted Answer

A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA. 
D) gel electrophoresis. 
E) gene probes. 
A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA. 
D) gel electrophoresis. 
E) gene probes.

Question 8

Transgenic animals are _____ modified organisms.

Accepted Answer

The answer of Transgenic animals are _____ modified organisms....

Question 9

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Accepted Answer

The answer of In recombinant DNA technique, an enzyme called...

Question 10

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Accepted Answer

A) palindromes. 
B) reverse transcriptase. 
C) restriction endonucleases. 
D) ligases. 
E) DNA polymerases. 
A) palindromes. 
B) reverse transcriptase. 
C) restriction endonucleases. 
D) ligases. 
E) DNA polymerases.

Question 11

Sequencing a gene automatically identifies which gene it is.

Accepted Answer

A) True 
 B)False

Question 12

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Accepted Answer

A) RNA promoters 
B) fluorescent nucleotides 
C) primers 
D) thermophilic polymerases 
E) endonucleases 
A) RNA promoters 
B) fluorescent nucleotides 
C) primers 
D) thermophilic polymerases 
E) endonucleases

Question 13

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Accepted Answer

A) True 
 B)False

Question 14

Some genes in bacteria are repressed by antisense RNA that binds to them.

Accepted Answer

A) True 
 B)False

Question 15

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

The answer of The size of DNA is often given...

Question 16

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called

Accepted Answer

A) cloning. 
B) gene therapy. 
C) antisense therapeutic. 
D) DNA fingerprinting. 
E) None of the choices are correct. 
A) cloning. 
B) gene therapy. 
C) antisense therapeutic. 
D) DNA fingerprinting. 
E) None of the choices are correct.

Question 17

Gene probes can be labeled for detection with

Accepted Answer

A) enzymes. 
B) fluorescent dyes. 
C) radioisotopes. 
D) All of the choices are correct. 
E) None of the choices are correct. 
A) enzymes. 
B) fluorescent dyes. 
C) radioisotopes. 
D) All of the choices are correct. 
E) None of the choices are correct.

Question 18

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

Accepted Answer

A) mitochondrial DNA 
B) chloroplast DNA 
C) rRNA 
D) mRNA 
A) mitochondrial DNA 
B) chloroplast DNA 
C) rRNA 
D) mRNA

Question 19

When DNA is heated to just below boiling (90oC - 95oC),

Accepted Answer

A) the sugar phosphate backbone of the DNA strands breaks. 
B) the sugar, phosphate and base come apart in each nucleotide. 
C) the helix unwinds but hydrogen bonds between the bases remain intact. 
D) the two DNA strands separate completely. 
E) Nothing happens until the boiling point is reached (100o
C) . 
A) the sugar phosphate backbone of the DNA strands breaks. 
B) the sugar, phosphate and base come apart in each nucleotide. 
C) the helix unwinds but hydrogen bonds between the bases remain intact. 
D) the two DNA strands separate completely. 
E) Nothing happens until the boiling point is reached (100o
C) .

Question 20

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Accepted Answer

The answer of The two common vectors used to transfer...

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

An example of gene therapy is the insertion of the gene for human growth hormone into E.coli cells.

A technique that separates a readable pattern of DNA fragments is

The primers in PCR are

The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called

Transformation and transfection are methods used to introduce DNA into host cells.

The Western Blot technique detects

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Transgenic animals are _____ modified organisms.

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are

Sequencing a gene automatically identifies which gene it is.

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Some genes in bacteria are repressed by antisense RNA that binds to them.

The size of DNA is often given in the number of _____ that it contains.

When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called

Gene probes can be labeled for detection with

Which of the following is less subject to degradation than is chromosomal DNA and is used as an evolutionary time clock?

When DNA is heated to just below boiling (90oC - 95oC),

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

Filters

When DNA is heated to just below boiling (90^oC - 95^oC),

The two common vectors used to transfer a piece of DNA into a cloning host are _ and _.