Question 1

Which of the following choices are true about the dyes fluorescein and rhodamine?

Accepted Answer

A) They can be used to label antibodies in immunofluorescence tests. 
B) They emit visible light in response to ultraviolet radiation. 
C) They are observed in the fluorescent microscope. 
D) They re used to identify pathogens of chlamydiosis, Legionnaires' disease, and others. 
E) All of the choices are ture. 
A) They can be used to label antibodies in immunofluorescence tests. 
B) They emit visible light in response to ultraviolet radiation. 
C) They are observed in the fluorescent microscope. 
D) They re used to identify pathogens of chlamydiosis, Legionnaires' disease, and others. 
E) All of the choices are ture. E

Question 2

Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?

Accepted Answer

A) Ouchterlony double diffusion 
B) Western blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test 
A) Ouchterlony double diffusion 
B) Western blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test D

Question 3

Serological testing always involves reactions between specific antibody and antigen.

Accepted Answer

A) True 
 B)False  True

Question 4

Flowcharts that offer two choices at each identifying level are called ___ keys.

Accepted Answer

Flowcharts that offer two choices at eac

Question 5

Describe three possible sampling sites and methods of collection for clinical laboratories.

Accepted Answer

1. Blood Collection from a Vein: One com

Question 6

The property of a test to detect even small amounts of antibodies or antigens that are test targets is:

Accepted Answer

A) cross-reactions. 
B) agglutination. 
C) precipitation. 
D) specificity. 
E) sensitivity. 
A) cross-reactions. 
B) agglutination. 
C) precipitation. 
D) specificity. 
E) sensitivity.

Question 7

Broad general characteristics on flowcharts can be misleading.

Accepted Answer

A) True 
 B)False

Question 8

Whole antigens are detected in which type of test?

Accepted Answer

A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity 
A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity

Question 9

Which technique used to diagnose a disease is also called the double diffusion method?

Accepted Answer

A) Ouchterlony 
B) VDRL test 
C) immunoelectrophoresis 
D) Western blot 
E) ELISA 
A) Ouchterlony 
B) VDRL test 
C) immunoelectrophoresis 
D) Western blot 
E) ELISA

Question 10

Describe the indirect ELISA test that is used as a screening test for HIV (AIDS) and include the following: a) sequence of procedures, b) reagents used, and c) detection and interpretation of a positive test.

Accepted Answer

The indirect ELISA test for HIV (AIDS) i

Question 11

In direct antigen testing, the reaction is generally seen with the:

Accepted Answer

A) electron microscope. 
B) naked eye. 
C) light microscope. 
D) API 20E miniature identification system. 
E) PCR reaction. 
A) electron microscope. 
B) naked eye. 
C) light microscope. 
D) API 20E miniature identification system. 
E) PCR reaction.

Question 12

Which of the following methods is categorized as a direct test in order to analyze a specimen?

Accepted Answer

A) Catalase test 
B) Gram stain 
C) Phage typing 
D) Antimicrobic sensitivity 
E) Production of gas 
A) Catalase test 
B) Gram stain 
C) Phage typing 
D) Antimicrobic sensitivity 
E) Production of gas

Question 13

Serological tests should have low sensitivity and specificity.

Accepted Answer

A) True 
 B)False

Question 14

The property of a test to detect only a certain antibody or antigen, and not to react with any others, is:

Accepted Answer

A) cross-reactions. 
B) agglutination. 
C) precipitation. 
D) specificity. 
E) sensitivity. 
A) cross-reactions. 
B) agglutination. 
C) precipitation. 
D) specificity. 
E) sensitivity.

Question 15

Identify, describe, and discuss the objectives of the three main categories of identifying infectious agents using specific examples.

Accepted Answer

The three main categories of identifying

Question 16

The indirect ELISA test detects ______ in a patient's blood.

Accepted Answer

A) antigen 
B) antibody 
C) microorganisms 
D) IgE only 
E) complement 
A) antigen 
B) antibody 
C) microorganisms 
D) IgE only 
E) complement

Question 17

In indirect fluorescent testing, fluorescent _____ are used to react with the Fc region of another, specific, serum antibody.

Accepted Answer

The answer of In indirect fluorescent testing, fluorescent _____ are...

Question 18

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

Accepted Answer

A) immunoelectrophoresis 
B) Ouchterlony 
C) complement fixation 
D) Western blot 
E) radioimmunoassay 
A) immunoelectrophoresis 
B) Ouchterlony 
C) complement fixation 
D) Western blot 
E) radioimmunoassay

Question 19

The Widal test is used for diagnosing salmonellosis.

Accepted Answer

A) True 
 B)False

Question 20

Test systems involving which of the following has greatly improved the specificity of immunological tests?

Accepted Answer

A) monoclonal antibodies 
B) electronics 
C) enzymes 
D) radioactivity 
E) All of the choices are correct. 
A) monoclonal antibodies 
B) electronics 
C) enzymes 
D) radioactivity 
E) All of the choices are correct.

Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

Which of the following choices are true about the dyes fluorescein and rhodamine?

Which test uses radioactive isotopes to label antibodies or antigens in order to detect minute amounts of corresponding antigen or antibody?

Serological testing always involves reactions between specific antibody and antigen.

Flowcharts that offer two choices at each identifying level are called ___ keys.

Describe three possible sampling sites and methods of collection for clinical laboratories.

The property of a test to detect even small amounts of antibodies or antigens that are test targets is:

Broad general characteristics on flowcharts can be misleading.

Whole antigens are detected in which type of test?

Which technique used to diagnose a disease is also called the double diffusion method?

Describe the indirect ELISA test that is used as a screening test for HIV (AIDS) and include the following: a) sequence of procedures, b) reagents used, and c) detection and interpretation of a positive test.

In direct antigen testing, the reaction is generally seen with the:

Which of the following methods is categorized as a direct test in order to analyze a specimen?

Serological tests should have low sensitivity and specificity.

The property of a test to detect only a certain antibody or antigen, and not to react with any others, is:

Identify, describe, and discuss the objectives of the three main categories of identifying infectious agents using specific examples.

The indirect ELISA test detects ______ in a patient's blood.

In indirect fluorescent testing, fluorescent _____ are used to react with the Fc region of another, specific, serum antibody.

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

The Widal test is used for diagnosing salmonellosis.

Test systems involving which of the following has greatly improved the specificity of immunological tests?

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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