Question 1

The acronym HPLC stands for

Accepted Answer

A) hydrophobic protein liquid chromatography. 
B) high performance liquid chromatography. 
C) hydrophilic partition liquid chromatography. 
D) high priced liquid chromatography. 
E) hydrostatic process liquid chromatography. 
A) hydrophobic protein liquid chromatography. 
B) high performance liquid chromatography. 
C) hydrophilic partition liquid chromatography. 
D) high priced liquid chromatography. 
E) hydrostatic process liquid chromatography.

Question 2

Since there are 20 standard amino acids, the number of possible linear polypeptides of length N can be expressed as:

Accepted Answer

A) n × 20 
B) 20n 
C) 20 × 10n 
D) n20 
E) n × 1020 
A) n × 20 
B) 20n 
C) 20 × 10n 
D) n20 
E) n × 1020

Question 3

The vast majority of polypeptides contain between ______ amino acid residues.

Accepted Answer

A) 10 and 50 
B) 50 and 100 
C) 100 and 1000 
D) 1000 and 2000 
E) 2000 and 34,000 
A) 10 and 50 
B) 50 and 100 
C) 100 and 1000 
D) 1000 and 2000 
E) 2000 and 34,000

Question 4

Matching
-If antibodies to the protein being assayed are available, a(n)______ can be carried out.

Accepted Answer

A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate 
A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate

Question 5

In two homologous proteins, which residue is most likely to replace a Glu residue as a conservative substitution?

Accepted Answer

A) Asp 
B) Trp 
C) Met 
D) Ile 
E) Lys 
A) Asp 
B) Trp 
C) Met 
D) Ile 
E) Lys

Question 6

Proteins are synthesized in vivo by the translation of

Accepted Answer

A) cDNA. 
B) tRNA. 
C) rRNA. 
D) exons. 
E) mRNA. 
A) cDNA. 
B) tRNA. 
C) rRNA. 
D) exons. 
E) mRNA.

Question 7

A first step in purifying a protein that was initially associated with fatty substances would be

Accepted Answer

A) Coomassie Brilliant Blue dye staining. 
B) analytical ultracentrifugation. 
C) ELISA. 
D) Western blotting. 
E) hydrophobic interaction chromatography. 
A) Coomassie Brilliant Blue dye staining. 
B) analytical ultracentrifugation. 
C) ELISA. 
D) Western blotting. 
E) hydrophobic interaction chromatography.

Question 8

Enzymes that hydrolyze the internal peptide bonds (not the peptide bonds of the terminal amino acids)of a protein are classified as

Accepted Answer

A) oxidoreductases. 
B) lyases. 
C) endopeptidases. 
D) nucleases. 
E) exopeptidases. 
A) oxidoreductases. 
B) lyases. 
C) endopeptidases. 
D) nucleases. 
E) exopeptidases.

Question 9

Matching
-______ chromatography is a method of fractionating a protein mixture according to differences in polarity.

Accepted Answer

A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate 
A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate

Question 10

One technique commonly used in protein purification is chromatography.
a.Explain briefly the general principle of column chromatography
b.Name four types of chromatography and indicate for each of these types the basis for separation (match types of chromatography with the properties that form the basis for separation).

Accepted Answer

a.In chromatography there are two phases

Question 11

The positive charge on proteins in electrospray ionization mass spectrometry is the result of

Accepted Answer

A) protons fired at the gas-phase protein molecules. 
B) protonated side chains of Asp and Glu residues. 
C) protonated side chains of Arg and Lys residues. 
D) a high pH. 
E) electrons fired at the gas-phase protein molecules. 
A) protons fired at the gas-phase protein molecules. 
B) protonated side chains of Asp and Glu residues. 
C) protonated side chains of Arg and Lys residues. 
D) a high pH. 
E) electrons fired at the gas-phase protein molecules.

Question 12

Edman degradation can be used to

Accepted Answer

A) identify the N-terminal amino acid of a polypeptide. 
B) identify the C-terminal amino acid of a polypeptide. 
C) separate the subunits of a multi-subunit protein. 
D) cleave a protein at specific sites. 
E) cleave disulfide bonds within a protein so that the individual polypeptides can be separated. 
A) identify the N-terminal amino acid of a polypeptide. 
B) identify the C-terminal amino acid of a polypeptide. 
C) separate the subunits of a multi-subunit protein. 
D) cleave a protein at specific sites. 
E) cleave disulfide bonds within a protein so that the individual polypeptides can be separated.

Question 13

The pK1, pK2, and pKR of the amino acid lysine are 2.2, 9.1, and 10.5, respectively.The pK1, pK2, and pKR of the amino acid arginine are 1.8, 9.0, and 12.5, respectively.A student at SDSU wants to use ion exchange chromatography to separate lysine from arginine.What pH is likely to work best for this separation?

Accepted Answer

A) 1.5 
B) 2.5 
C) 5.5 
D) 7.5 
E) 10.5 
A) 1.5 
B) 2.5 
C) 5.5 
D) 7.5 
E) 10.5

Question 14

Matching
-One of the reasons the primary structure is important for a protein is that it determines the ______ the molecule adopts in aqueous solutions.

Accepted Answer

A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate 
A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate

Question 15

Proteins can vary in size from approximately 40 to 34,000 amino acids.
a.Why is there a lower limit to the size of proteins?
b.Why is there an upper limit to the size of polypeptides?

Accepted Answer

a.Polypeptides shorter than 40 residues

Question 16

Which of the following amino acids would be last to elute at pH 8.0 from an anion-exchange column?

Accepted Answer

A) lysine 
B) alanine 
C) glutamic acid 
D) asparagine 
E) glycine 
A) lysine 
B) alanine 
C) glutamic acid 
D) asparagine 
E) glycine

Question 17

Matching
-In order for DEAE to act as an anion exchanger, it must have a ______.

Accepted Answer

A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate 
A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate

Question 18

The quantitation of proteins due to their absorbance at ~280 nm (UV region)is due to the large absorbtivity of the ________ amino acids.

Accepted Answer

A) anionic 
B) dansylated 
C) cleaved 
D) polar 
E) aromatic 
A) anionic 
B) dansylated 
C) cleaved 
D) polar 
E) aromatic

Question 19

Which of these techniques uses antibodies to detect very small amounts of specific proteins following separation by SDS-PAGE.

Accepted Answer

A) immunoblotting 
B) silverstaining 
C) Coomassie Brilliant Blue staining 
D) ELISA 
E) RIA 
A) immunoblotting 
B) silverstaining 
C) Coomassie Brilliant Blue staining 
D) ELISA 
E) RIA

Question 20

Matching
-Either dansyl chloride or Edman's reagent can be used to identify the ______ of a protein.

Accepted Answer

A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate 
A) electrophoresis 
B) hydrophobic interaction 
C) enzyme-linked immunosorbent assay 
D) three-dimensional shape 
E) N-terminal amino acid
F)negative charge
G)nucleases
H)chromophore
I)foaming
J)high level expression
K)2-mercaptoethanol
L)positive charge
M)cation exchange
N)pI
O)chymotrypsin
P)C-terminal amino acid
Q)Sodium dodecyl sulfate

Exam 5: Proteins: Primary Structure

The acronym HPLC stands for

Since there are 20 standard amino acids, the number of possible linear polypeptides of length N can be expressed as:

The vast majority of polypeptides contain between ______ amino acid residues.

Matching -If antibodies to the protein being assayed are available, a(n)______ can be carried out.

In two homologous proteins, which residue is most likely to replace a Glu residue as a conservative substitution?

Proteins are synthesized in vivo by the translation of

A first step in purifying a protein that was initially associated with fatty substances would be

Enzymes that hydrolyze the internal peptide bonds (not the peptide bonds of the terminal amino acids)of a protein are classified as

Matching -______ chromatography is a method of fractionating a protein mixture according to differences in polarity.

The positive charge on proteins in electrospray ionization mass spectrometry is the result of

Edman degradation can be used to

Matching -One of the reasons the primary structure is important for a protein is that it determines the ______ the molecule adopts in aqueous solutions.

Proteins can vary in size from approximately 40 to 34,000 amino acids. a.Why is there a lower limit to the size of proteins? b.Why is there an upper limit to the size of polypeptides?

Which of the following amino acids would be last to elute at pH 8.0 from an anion-exchange column?

Matching -In order for DEAE to act as an anion exchanger, it must have a ______.

The quantitation of proteins due to their absorbance at ~280 nm (UV region)is due to the large absorbtivity of the ________ amino acids.

Which of these techniques uses antibodies to detect very small amounts of specific proteins following separation by SDS-PAGE.

Matching -Either dansyl chloride or Edman's reagent can be used to identify the ______ of a protein.

Introduction to the Chemistry of Life

Water

Nucleotides, Nucleic Acids, and Genetic Information

Amino Acids

Proteins: Three-Dimensional Structure

Protein Function Part I: Myoglobin and Hemoglobin

Carbohydrates

Lipids and Biological Membranes

Membrane Transport

Enzyme Catalysis

Enzyme Kinetics, Inhibition and Control

Biochemical Signaling

Introduction to Metabolism

Glycogen Metabolism and Gluconeogenesis

Glucose Catabolism

Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Mammalian Fuel Metabolism: Integration and Regulation

Nucleotide Metabolism

Nucleic Acid Structure

Dna Replication, Repair and Recombination

Transcription and Rna Processing

Translation

Regulation of Gene Expression

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