Question 1

Which process transfers information from DNA to RNA?

Accepted Answer

A)  Replication 
B)  Transcription 
C)  Translation 
D)  Splicing 
E)  None of these 
A)  Replication 
B)  Transcription 
C)  Translation 
D)  Splicing 
E)  None of these B

Question 2

Which of the following characteristics of transcriptional termination is indicative of rho-dependent activity?

Accepted Answer

A)  Hairpin structure 
B)  Protein moving 3'->5' on the growing mRNA 
C)  Six or more GU basepairs 
D)  Protein mediated "pulling" of the nascent RNA chain from the transcription bubble 
E)  All of these 
A)  Hairpin structure 
B)  Protein moving 3'->5' on the growing mRNA 
C)  Six or more GU basepairs 
D)  Protein mediated "pulling" of the nascent RNA chain from the transcription bubble 
E)  All of these D

Question 3

A basepair sequence preceding the transcription initiation site is known as a/an:

Accepted Answer

A)  Upstream sequence 
B)  Downstream sequence 
C)  Promoter 
D)  Operator 
E)  Enhancer 
A)  Upstream sequence 
B)  Downstream sequence 
C)  Promoter 
D)  Operator 
E)  Enhancer A

Question 4

Which of the following E.coli RNA polymerase subunits are responsible only for promoter specific transcription in vitro?

Accepted Answer

A)    
B)    
C)    
D)   
E)    
A)    
B)    
C)    
D)   
E)

Question 5

Small RNA molecules that function as adaptors between amino acids and the codons in mRNA during translation are known as:

Accepted Answer

A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
E)  pRNA 
A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
E)  pRNA

Question 6

RNA polymerase binds to a specific nucleotide sequence on the template strand known as a/an:

Accepted Answer

A)  Operator 
B)  Promoter 
C)  Enhancer 
D)  Silencer 
E)  None of these 
A)  Operator 
B)  Promoter 
C)  Enhancer 
D)  Silencer 
E)  None of these

Question 7

Which of the following are biochemical features of RNA polymerase? 1.5'->3' polymerase activity.
2)Recognition of a promoter sequence to initiate transcription with help from factors.
3)Local unwinding of DNA,called a transcription bubble,provides a template for enzymatic activity.

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  2 and 3 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  2 and 3 
E)  All of these

Question 8

Compare,in detail,the three forms of introns removal in eukaryotes.

Accepted Answer

The excision of introns from tRNA precur

Question 9

Which type of RNA are structural components of spliceosomes,the nuclear structures that excise introns from nuclear genes?

Accepted Answer

A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
E)  pRNA 
A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
E)  pRNA

Question 10

Introns in mRNA are removed by:

Accepted Answer

A)  The concerted action of a splicing endonuclease and Ligase 
B)  Autocatalytical splicing 
C)  Ribonucleoprotein structures called spliceosomes 
D)  All of these 
E)  none of these 
A)  The concerted action of a splicing endonuclease and Ligase 
B)  Autocatalytical splicing 
C)  Ribonucleoprotein structures called spliceosomes 
D)  All of these 
E)  none of these

Question 11

Which of the following is a form of RNA editing? 1.The polyadenylation of the pre-mRNA sequence
2)The changing of the structures of individual bases
3)The insertion or deletion of uridine monophosphate residues

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  2 and 3 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  2 and 3

Question 12

The RNA molecules that are intermediaries between DNA and polypeptides are known as:

Accepted Answer

A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
E)  pRNA 
A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
E)  pRNA

Question 13

The 3¢ ends of RNA transcripts synthesized by RNA polymerase II are produced by: 1.Endonucleolytic cleavage of the primary transcripts
2)Termination of transcription
3)The addition of the 5' 7-methyl guanosine cap

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 14

In which direction does RNA synthesis occur?

Accepted Answer

A)  5' ® 3' 
B)  3' ® 5' 
C)  1' ® 3' 
D)  5' ® 3'and 3' ® 5' 
E)  RNA synthesis occurs randomly without a specific direction 
A)  5' ® 3' 
B)  3' ® 5' 
C)  1' ® 3' 
D)  5' ® 3'and 3' ® 5' 
E)  RNA synthesis occurs randomly without a specific direction

Question 15

What is the purpose for the addition of the 5' 7-methyl guanosine cap on eukaryotic pre-mRNA molecules?

Accepted Answer

A)  It prevents the degredation of the sequence by nucleases 
B)  It prevents the degredation of the sequence by lysosomes 
C)  It guides the pre-mRNA sequence out of the plasma membrane 
D)  All of these 
E)  None of these 
A)  It prevents the degredation of the sequence by nucleases 
B)  It prevents the degredation of the sequence by lysosomes 
C)  It guides the pre-mRNA sequence out of the plasma membrane 
D)  All of these 
E)  None of these

Question 16

Compare and contrast rho-independent termination and rho-dependent termination during transcription in prokaryotes

Accepted Answer

There are two types of transcription ter

Question 17

Introns in tRNA are removed by:

Accepted Answer

A)  The concerted action of a splicing endonuclease and Ligase 
B)  Autocatalytical splicing 
C)  Ribonucleoprotein structures called spliceosomes 
D)  All of these 
E)  none of these 
A)  The concerted action of a splicing endonuclease and Ligase 
B)  Autocatalytical splicing 
C)  Ribonucleoprotein structures called spliceosomes 
D)  All of these 
E)  none of these

Question 18

The initiation of transcription is characterized by:

Accepted Answer

A)  The binding of RNA polymerase to the promoter region of the template DNA 
B)  The binding of DNA polymerase to the operator region of the template RNA 
C)  The binding of Helicase to the operator region of the template RNA 
D)  The binding of Ligase to the promoter region of the template DNA 
E)  None of these 
A)  The binding of RNA polymerase to the promoter region of the template DNA 
B)  The binding of DNA polymerase to the operator region of the template RNA 
C)  The binding of Helicase to the operator region of the template RNA 
D)  The binding of Ligase to the promoter region of the template DNA 
E)  None of these

Question 19

RNA synthesis takes place within a locally unwound segment of DNA,sometimes called the:

Accepted Answer

A)  Translation bubble 
B)  Replication bubble 
C)  Transcription bubble 
D)  DNA/RNA hybrid 
E)  Operator bubble 
A)  Translation bubble 
B)  Replication bubble 
C)  Transcription bubble 
D)  DNA/RNA hybrid 
E)  Operator bubble

Question 20

During a research event,the s subunit is removed from the RNA polymerase enzyme.How will this affect the initiation of transcription?

Accepted Answer

A)  Transcription will initiate and proceed normally 
B)  Transcription initiation will be stopped 
C)  Transcription will initiate at random spots along the template 
D)  All of these 
E)  None of these 
A)  Transcription will initiate and proceed normally 
B)  Transcription initiation will be stopped 
C)  Transcription will initiate at random spots along the template 
D)  All of these 
E)  None of these

Exam 11: Transcription And RNA Processing

Which process transfers information from DNA to RNA?

Which of the following characteristics of transcriptional termination is indicative of rho-dependent activity?

A basepair sequence preceding the transcription initiation site is known as a/an:

Which of the following E.coli RNA polymerase subunits are responsible only for promoter specific transcription in vitro?

Small RNA molecules that function as adaptors between amino acids and the codons in mRNA during translation are known as:

RNA polymerase binds to a specific nucleotide sequence on the template strand known as a/an:

Which of the following are biochemical features of RNA polymerase? 1.5'->3' polymerase activity. 2)Recognition of a promoter sequence to initiate transcription with help from factors. 3)Local unwinding of DNA,called a transcription bubble,provides a template for enzymatic activity.

Compare,in detail,the three forms of introns removal in eukaryotes.

Which type of RNA are structural components of spliceosomes,the nuclear structures that excise introns from nuclear genes?

Introns in mRNA are removed by:

Which of the following is a form of RNA editing? 1.The polyadenylation of the pre-mRNA sequence 2)The changing of the structures of individual bases 3)The insertion or deletion of uridine monophosphate residues

The RNA molecules that are intermediaries between DNA and polypeptides are known as:

The 3¢ ends of RNA transcripts synthesized by RNA polymerase II are produced by: 1.Endonucleolytic cleavage of the primary transcripts 2)Termination of transcription 3)The addition of the 5' 7-methyl guanosine cap

In which direction does RNA synthesis occur?

What is the purpose for the addition of the 5' 7-methyl guanosine cap on eukaryotic pre-mRNA molecules?

Compare and contrast rho-independent termination and rho-dependent termination during transcription in prokaryotes

Introns in tRNA are removed by:

The initiation of transcription is characterized by:

RNA synthesis takes place within a locally unwound segment of DNA,sometimes called the:

During a research event,the s subunit is removed from the RNA polymerase enzyme.How will this affect the initiation of transcription?

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Variation In Chromosome Number And Structure

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Regulation Of Gene Expression In Prokaryotes

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