Question 1

Which of the following enzymes is required for sequencing a gene?

Accepted Answer

A)  RNA polymerases 
B)  DNA gyrases 
C)  Restriction endonucleases 
D)  Lactases 
E)  None of these 
A)  RNA polymerases 
B)  DNA gyrases 
C)  Restriction endonucleases 
D)  Lactases 
E)  None of these C

Question 2

When pASH1 is digested by various restriction enzymes and combinations thereof,the following bands are observed.Construct a restriction enzyme map with this information.

Accepted Answer

The answer of When pASH1 is digested by various restriction...

Question 3

A physical map of a chromosome can be created by:

Accepted Answer

A)  Mapping the restriction cleavage sites in the chromosome 
B)  Using electron microscopy 
C)  Using western blotting 
D)  Using recombination experiments 
E)  All of these 
A)  Mapping the restriction cleavage sites in the chromosome 
B)  Using electron microscopy 
C)  Using western blotting 
D)  Using recombination experiments 
E)  All of these A

Question 4

Which of the following techniques allow researchers to isolate and characterize DNA sequences?

Accepted Answer

A)  Recombinant DNA techniques 
B)  Gene cloning 
C)  DNA amplification 
D)  Gene cloning and DNA amplification 
E)  All of these 
A)  Recombinant DNA techniques 
B)  Gene cloning 
C)  DNA amplification 
D)  Gene cloning and DNA amplification 
E)  All of these

Question 5

Which of the following statements about cosmid vectors is false?

Accepted Answer

A)  They can accept DNA inserts of 35-45kb in size 
B)  They can replicate autonomously like a plasmid 
C)  Their DNA is packaged in the heads of lambda phage particles 
D)  They do not contain an origin of replication or a selectable marker gene 
E)  All of these 
A)  They can accept DNA inserts of 35-45kb in size 
B)  They can replicate autonomously like a plasmid 
C)  Their DNA is packaged in the heads of lambda phage particles 
D)  They do not contain an origin of replication or a selectable marker gene 
E)  All of these

Question 6

If a PCR reaction begins using only one double stranded fragment of DNA,how many fragments will be produced after 12 cycles?

Accepted Answer

A)  2 
B)  12 
C)  256 
D)  1024 
E)  4096 
A)  2 
B)  12 
C)  256 
D)  1024 
E)  4096

Question 7

cDNA libraries contain only:

Accepted Answer

A)  The non coding regions of expressed genes 
B)  The coding regions of expressed genes 
C)  The non coding and coding regions of non-expressed genes 
D)  All of these 
E)  None of these 
A)  The non coding regions of expressed genes 
B)  The coding regions of expressed genes 
C)  The non coding and coding regions of non-expressed genes 
D)  All of these 
E)  None of these

Question 8

Which of the following is an advantage that the cosmid vector possesses?
1)Can replicate autonomously in E.coli
2)Has the packaging capacity of lambda chromosome
3)Efficient transformation in E.coli

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 3 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 3 
E)  All of these

Question 9

If you were constructing the first strand of a cDNA library,you would need all of the following except:

Accepted Answer

A)  DNA Ligase 
B)  DNA template 
C)  RNA template 
D)  Poly-T oligomers 
E)  Reverse transcriptase 
A)  DNA Ligase 
B)  DNA template 
C)  RNA template 
D)  Poly-T oligomers 
E)  Reverse transcriptase

Question 10

A disadvantage of l cloning vectors is:

Accepted Answer

A)  An inability to accommodate eukaryotic DNA 
B)  The absence of unique restriction sites 
C)  An inability to be packaged in vitro 
D)  A strict limit on the insert size (no larger than 10kb) 
E)  The instability of large foreign DNA inserts 
A)  An inability to accommodate eukaryotic DNA 
B)  The absence of unique restriction sites 
C)  An inability to be packaged in vitro 
D)  A strict limit on the insert size (no larger than 10kb) 
E)  The instability of large foreign DNA inserts

Question 11

Which of the following is true about Taq polymerase?
1)It does not contain a built-in 3¢ ® 5¢ proofreading activity
2)It produces a higher than normal frequency of replication errors
3)It is derived from a bacteria known as Thermus aquaticus

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 3 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 3 
E)  All of these

Question 12

Which of the following is a method by which a gene can be isolated from a DNA library?

Accepted Answer

A)  In situ colony hybridization 
B)  Genetic complementation 
C)  Genetic selection 
D)  In situ plaque hybridization 
E)  All of these 
A)  In situ colony hybridization 
B)  Genetic complementation 
C)  Genetic selection 
D)  In situ plaque hybridization 
E)  All of these

Question 13

A cosmid can best be described as a hybrid of:

Accepted Answer

A)  A plasmid and a bacteriophage 
B)  A plasmid and a BAC 
C)  A bacteriophage and a phagemid 
D)  A phagemid and a BAC 
E)  None of these 
A)  A plasmid and a bacteriophage 
B)  A plasmid and a BAC 
C)  A bacteriophage and a phagemid 
D)  A phagemid and a BAC 
E)  None of these

Question 14

The purpose of the primer sequence in PCR is to:

Accepted Answer

A)  Provide a promoter sequence for DNA polymerase 
B)  Provide a free 3'-OH end required for covalent extension 
C)  Allow for efficient denaturation of the double stranded DNA 
D)  Keep the DNA denatured after the temperature is reduced 
E)  All of these 
A)  Provide a promoter sequence for DNA polymerase 
B)  Provide a free 3'-OH end required for covalent extension 
C)  Allow for efficient denaturation of the double stranded DNA 
D)  Keep the DNA denatured after the temperature is reduced 
E)  All of these

Question 15

Which of the following types of restriction enzymes cleave DNA at only specific nucleotide sequences known as restriction sites,regardless of the source of the DNA?

Accepted Answer

A)  Type I 
B)  Type II 
C)  Type III 
D)  Type IV 
E)  Type V 
A)  Type I 
B)  Type II 
C)  Type III 
D)  Type IV 
E)  Type V

Question 16

The intragenic complementation between the amino terminal fragment of b-galactosidase and the defective DM15 polypeptide which permits the Xgal color test to be utilized without placing the entire lacZ gene in the pUC vectors is called:

Accepted Answer

A)  Forced cloning 
B)  a-complementation 
C)  Blue/white color selection 
D)  In situ hybridization 
E)  Complementation screening 
A)  Forced cloning 
B)  a-complementation 
C)  Blue/white color selection 
D)  In situ hybridization 
E)  Complementation screening

Question 17

Which of the following is the correct order of steps in PCR?

Accepted Answer

A)  Extension of DNA, denaturation of DNA, annealing of primers 
B)  Denaturation of DNA, extension of DNA, annealing of primers 
C)  Denaturation of DNA, annealing of primers, extension of DNA 
D)  Annealing of primers, extension of DNA, denaturation of DNA 
E)  Annealing of primers, denaturation of DNA, extension of DNA 
A)  Extension of DNA, denaturation of DNA, annealing of primers 
B)  Denaturation of DNA, extension of DNA, annealing of primers 
C)  Denaturation of DNA, annealing of primers, extension of DNA 
D)  Annealing of primers, extension of DNA, denaturation of DNA 
E)  Annealing of primers, denaturation of DNA, extension of DNA

Question 18

If you wanted to identify and characterize a protein in a cellular extract,which of the following techniques would you use?

Accepted Answer

A)  Southern Blotting 
B)  Northern Blotting 
C)  Western Blotting 
D)  Southern Blotting and Western Blotting 
E)  All of these 
A)  Southern Blotting 
B)  Northern Blotting 
C)  Western Blotting 
D)  Southern Blotting and Western Blotting 
E)  All of these

Question 19

A cluster of unique restriction sites in a cloning vector is often referred to as a/an:

Accepted Answer

A)  Polylinker 
B)  Polycloning site 
C)  Unilinker 
D)  Polylinker and Polycloning site 
E)  All of these 
A)  Polylinker 
B)  Polycloning site 
C)  Unilinker 
D)  Polylinker and Polycloning site 
E)  All of these

Question 20

Small self replicating genetic elements used to clone genes are known as:

Accepted Answer

A)  Cloner sections 
B) Cloning vectors 
C)  Cloning sequences 
D)  Restriction enzymes 
E)  Silencers 
A)  Cloner sections 
B) Cloning vectors 
C)  Cloning sequences 
D)  Restriction enzymes 
E)  Silencers

Exam 14: The Techniques Of Molecular Genetics

Which of the following enzymes is required for sequencing a gene?

When pASH1 is digested by various restriction enzymes and combinations thereof,the following bands are observed.Construct a restriction enzyme map with this information.

A physical map of a chromosome can be created by:

Which of the following techniques allow researchers to isolate and characterize DNA sequences?

Which of the following statements about cosmid vectors is false?

If a PCR reaction begins using only one double stranded fragment of DNA,how many fragments will be produced after 12 cycles?

cDNA libraries contain only:

Which of the following is an advantage that the cosmid vector possesses? 1)Can replicate autonomously in E.coli 2)Has the packaging capacity of lambda chromosome 3)Efficient transformation in E.coli

If you were constructing the first strand of a cDNA library,you would need all of the following except:

A disadvantage of l cloning vectors is:

Which of the following is true about Taq polymerase? 1)It does not contain a built-in 3¢ ® 5¢ proofreading activity 2)It produces a higher than normal frequency of replication errors 3)It is derived from a bacteria known as Thermus aquaticus

Which of the following is a method by which a gene can be isolated from a DNA library?

A cosmid can best be described as a hybrid of:

The purpose of the primer sequence in PCR is to:

Which of the following types of restriction enzymes cleave DNA at only specific nucleotide sequences known as restriction sites,regardless of the source of the DNA?

The intragenic complementation between the amino terminal fragment of b-galactosidase and the defective DM15 polypeptide which permits the Xgal color test to be utilized without placing the entire lacZ gene in the pUC vectors is called:

Which of the following is the correct order of steps in PCR?

If you wanted to identify and characterize a protein in a cellular extract,which of the following techniques would you use?

A cluster of unique restriction sites in a cloning vector is often referred to as a/an:

Small self replicating genetic elements used to clone genes are known as:

The Science Of Genetics

Cellular Reproduction

Mendelism The Basic Principles Of Inheritance

Extensions Of Mendelism

The Chromosomal Basis Of Mendelism

Variation In Chromosome Number And Structure

Linkage,Crossing Over,And Chromosome Mapping In …

The Genetics Of Bacteria And Their Viruses

DNA And The Molecular Structure Of Chromosomes

Replication Of DNA And Chromosomes

Transcription And RNA Processing

Translation And The Genetic Code

Mutation,DNA Repair,And Recombination

Genomics

Applications Of Molecular Genetics

Transposable Genetics Elements

Regulation Of Gene Expression In Prokaryotes

Regulation Of Gene Expression In Eukaryotes

The Genetic Control Of Animal Development

The Genetic Basis Of Cancer

Inheritance Of Complex Traits

Population Genetics

Evolutionary Genetics

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