Question 1

Which of the following RNA polymerases catalyze the initiation of transcription in most eukaryotic genes?

Accepted Answer

A)  RNA Polymerase 1 
B)  RNA Polymerase 2 
C)  RNA Polymerase 3 
D)  RNA Polymerase 4 
E)  RNA Polymerase 5 
A)  RNA Polymerase 1 
B)  RNA Polymerase 2 
C)  RNA Polymerase 3 
D)  RNA Polymerase 4 
E)  RNA Polymerase 5

Question 2

The polyadenylation of the pre-mRNA sequences in eukaryotes occurs during which stage of eukaryotic transcription?

Accepted Answer

A)  Initation 
B)  Elongation 
C)  Termination 
D)  Post-transcriptional processing 
E)  It can occur at any time 
A)  Initation 
B)  Elongation 
C)  Termination 
D)  Post-transcriptional processing 
E)  It can occur at any time

Question 3

Briefly describe the process of initiation for RNA transcription in prokaryotes

Accepted Answer

Initiation of RNA chains involves three

Question 4

Consider the DNA template 3'-AAATTTTAGCCA-5'.When transcribed,which of the following is the correct resulting transcript?

Accepted Answer

A)  5'-TTTAAATCGGT-3' 
B)  5'-UUUAAAUGCCA-3' 
C)  5'-UUUAAAUCGGU-3' 
D)  3'-TTTAAATCGGT-5' 
E)  3'-AAATTTTAGCCA-5' 
A)  5'-TTTAAATCGGT-3' 
B)  5'-UUUAAAUGCCA-3' 
C)  5'-UUUAAAUCGGU-3' 
D)  3'-TTTAAATCGGT-5' 
E)  3'-AAATTTTAGCCA-5'

Question 5

Which of the following is the RNA polymerase that is used for transcription in eukaryotes 1.RNA Polymerase 1
2)RNA Polymerase 2
3)RNA Polymerase 3

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 6

The addition of the 5' 7-methyl guanosine (7-MG)cap occurs during which stage of eukaryotic transcription?

Accepted Answer

A)  Initiation 
B)  Elongation 
C)  Termination 
D)  Post-transcriptional processing 
E)  It can occur at any time 
A)  Initiation 
B)  Elongation 
C)  Termination 
D)  Post-transcriptional processing 
E)  It can occur at any time

Question 7

Noncoding sequences found in eukaryotic genes that interrupt the coding sequences are known as:

Accepted Answer

A)  Introns 
B)  Exons 
C)  Spliceosomes 
D)  Pre-mRNA 
E)  None of these 
A)  Introns 
B)  Exons 
C)  Spliceosomes 
D)  Pre-mRNA 
E)  None of these

Question 8

Which process transfers information from RNA molecules to proteins?

Accepted Answer

A)  Replication 
B)  Transcription 
C)  Translation 
D)  Splicing 
E)  None of these 
A)  Replication 
B)  Transcription 
C)  Translation 
D)  Splicing 
E)  None of these

Question 9

Which statement is correct about E.coli promoters:

Accepted Answer

A)  Most promoters have a high degree of sequence similarity. 
B)  -10 and -35 regions show the most conservation between promoters. 
C)  The GC-rich -10 region facilitates the localized unwinding of DNA . 
D)  Distance between the -10 and -35 regions is moderately conserved from 10 to 30 nucleotides in length 
E)  None of these statements are correct 
A)  Most promoters have a high degree of sequence similarity. 
B)  -10 and -35 regions show the most conservation between promoters. 
C)  The GC-rich -10 region facilitates the localized unwinding of DNA . 
D)  Distance between the -10 and -35 regions is moderately conserved from 10 to 30 nucleotides in length 
E)  None of these statements are correct

Question 10

The function of the core enzyme of RNA Polymerase is:

Accepted Answer

A)  To recognize and bind RNA polymerase to the transcription initiation or promoter sites in DNA 
B)  To recognize and bind DNA polymerase to the transcription initiation or promoter sites in RNA 
C)  catalyze RNA synthesis from DNA templates in vitro 
D)  To catalyze DNA synthesis from DNA templates in vitro 
E)  To catalyze DNA synthesis from RNA templates in vitro 
A)  To recognize and bind RNA polymerase to the transcription initiation or promoter sites in DNA 
B)  To recognize and bind DNA polymerase to the transcription initiation or promoter sites in RNA 
C)  catalyze RNA synthesis from DNA templates in vitro 
D)  To catalyze DNA synthesis from DNA templates in vitro 
E)  To catalyze DNA synthesis from RNA templates in vitro

Question 11

The sigma subunit initially recognizes and binds to which of the following sequences?

Accepted Answer

A)  -10 
B)  -35 
C)  -50 
D)  +10 
E)  +35 
A)  -10 
B)  -35 
C)  -50 
D)  +10 
E)  +35

Question 12

How many strands of DNA are used as a template during the process of transcription?

Accepted Answer

A)  One 
B)  Two 
C)  Three 
D)  Four 
E)  None of these 
A)  One 
B)  Two 
C)  Three 
D)  Four 
E)  None of these

Question 13

The covalent extension of a growing RNA chain takes place in which of the following locations?

Accepted Answer

A)  Promoter 
B)  Operator 
C)  Transcription bubble 
D)  Replication bubble 
E)  Enhancer 
A)  Promoter 
B)  Operator 
C)  Transcription bubble 
D)  Replication bubble 
E)  Enhancer

Question 14

What name is given to the short (20 to 22-nucleotide)single-stranded RNAs that are cleaved from hairpin-shaped precursors and block the expression of complementary mRNAs by either causing their degradation or repressing their translation?

Accepted Answer

A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
E)  pRNA 
A)  tRNA 
B)  mRNA 
C)  rRNA 
D)  sRNA 
E)  pRNA

Question 15

mRNA strands that will specify amino acids in the protein gene product are also known as:

Accepted Answer

A)  Coding strands 
B)  Sense strands 
C)  Antisense strands 
D)  Coding strands and Sense strands 
E)  Coding strands and Antisense strands 
A)  Coding strands 
B)  Sense strands 
C)  Antisense strands 
D)  Coding strands and Sense strands 
E)  Coding strands and Antisense strands

Question 16

During transcription the newly formed complementary strand of RNA is known as the:

Accepted Answer

A)  Translate 
B)  Transcript 
C)  cRNA 
D)  cDNA 
E)  Genetic Code 
A)  Translate 
B)  Transcript 
C)  cRNA 
D)  cDNA 
E)  Genetic Code

Question 17

In eukaryotes,when RNA Polymerase II initiates transcription: 1.Basal transcription factors,designated TFIX (X is a letter designating individual factors),are required.
2)TFIID is the first protein to bind the promoter.
3)TFIIA catalyzes the unwinding of the DNA helix

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 18

Which part of the eukaryotic cell is the location of transcription?

Accepted Answer

A)  Nucleus 
B)  Mitochondria 
C)  Ribosome 
D)  Cytosol 
E)  Lysosome 
A)  Nucleus 
B)  Mitochondria 
C)  Ribosome 
D)  Cytosol 
E)  Lysosome

Question 19

Which of the following is a true statement regarding the transfer of information from DNA to RNA. 1.It is always irreversible
2)It occurs during a process known as translation
3)It is sometimes reversible

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  2 and 3 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  2 and 3

Question 20

Which of the following is the correct order of the steps of transcription?

Accepted Answer

A)  Initiation, termination, elongation 
B)  Initiation, transcription, elongation 
C)  Initiation, elongation, termination 
D)  Elongation, transcription, termination 
E)  Termination, elongation, initiation 
A)  Initiation, termination, elongation 
B)  Initiation, transcription, elongation 
C)  Initiation, elongation, termination 
D)  Elongation, transcription, termination 
E)  Termination, elongation, initiation

Exam 11: Transcription And RNA Processing

Which of the following RNA polymerases catalyze the initiation of transcription in most eukaryotic genes?

The polyadenylation of the pre-mRNA sequences in eukaryotes occurs during which stage of eukaryotic transcription?

Briefly describe the process of initiation for RNA transcription in prokaryotes

Consider the DNA template 3'-AAATTTTAGCCA-5'.When transcribed,which of the following is the correct resulting transcript?

Which of the following is the RNA polymerase that is used for transcription in eukaryotes 1.RNA Polymerase 1 2)RNA Polymerase 2 3)RNA Polymerase 3

The addition of the 5' 7-methyl guanosine (7-MG)cap occurs during which stage of eukaryotic transcription?

Noncoding sequences found in eukaryotic genes that interrupt the coding sequences are known as:

Which process transfers information from RNA molecules to proteins?

Which statement is correct about E.coli promoters:

The function of the core enzyme of RNA Polymerase is:

The sigma subunit initially recognizes and binds to which of the following sequences?

How many strands of DNA are used as a template during the process of transcription?

The covalent extension of a growing RNA chain takes place in which of the following locations?

What name is given to the short (20 to 22-nucleotide)single-stranded RNAs that are cleaved from hairpin-shaped precursors and block the expression of complementary mRNAs by either causing their degradation or repressing their translation?

mRNA strands that will specify amino acids in the protein gene product are also known as:

During transcription the newly formed complementary strand of RNA is known as the:

In eukaryotes,when RNA Polymerase II initiates transcription: 1.Basal transcription factors,designated TFIX (X is a letter designating individual factors),are required. 2)TFIID is the first protein to bind the promoter. 3)TFIIA catalyzes the unwinding of the DNA helix

Which part of the eukaryotic cell is the location of transcription?

Which of the following is a true statement regarding the transfer of information from DNA to RNA. 1.It is always irreversible 2)It occurs during a process known as translation 3)It is sometimes reversible

Which of the following is the correct order of the steps of transcription?

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