Question 1

Which of the following statements regarding DNA fingerprinting is true

Accepted Answer

A)  DNA fingerprinting has the drawback that it requires extremely large quantities of DNA for analysis. 
B)  Inbreeding within a population decreases the probability of finding identical fingerprints between individuals. 
C)  An advantage of the technique is that data obtained from one population can easily be extrapolated to another population. 
D)  DNA fingerprinting is applicable in all cases of questionable identity except in paternity cases. 
E)  A prerequisite of the technique is the availability of information about the frequency of the polymorphisms in the population. 
A)  DNA fingerprinting has the drawback that it requires extremely large quantities of DNA for analysis. 
B)  Inbreeding within a population decreases the probability of finding identical fingerprints between individuals. 
C)  An advantage of the technique is that data obtained from one population can easily be extrapolated to another population. 
D)  DNA fingerprinting is applicable in all cases of questionable identity except in paternity cases. 
E)  A prerequisite of the technique is the availability of information about the frequency of the polymorphisms in the population.

Question 2

How is hGH produced for pharmaceutical use by using E.coli as a producer?

Accepted Answer

To obtain expression in E.coli,the hGH c

Question 3

Which of the following can be attributed to the presence of unstable trinucleotide repeats?

Accepted Answer

A)  Huntington's disease 
B)  Fragile X syndrome 
C)  Mytonic Dystrophy 
D)  Huntington's Disease and Fragile X Syndrome 
E)  All of these 
A)  Huntington's disease 
B)  Fragile X syndrome 
C)  Mytonic Dystrophy 
D)  Huntington's Disease and Fragile X Syndrome 
E)  All of these

Question 4

When confirming paternity it would be best to use:
1)Multiple numbers of probes so that the genome can be categorized more accurately
2)Multiple potential father's DNA samples so as to rule out infidelity
3)Unknown samples of DNA to prevent bias

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 5

Which of the following is used to make cheese?

Accepted Answer

A)  Insulin 
B)  hGH 
C)  Erythropoietin 
D)  Renning 
E)  Interferon 
A)  Insulin 
B)  hGH 
C)  Erythropoietin 
D)  Renning 
E)  Interferon

Question 6

Which of the following is a common method of delivery used for somatic cell gene therapy?

Accepted Answer

A)  Viral vectors 
B)  Enzyme linked immunoglobin vectors 
C)  Cosmid vectors 
D)  Viral vectors and Enzyme linked immunoglobulin vectors 
E)  All of these 
A)  Viral vectors 
B)  Enzyme linked immunoglobin vectors 
C)  Cosmid vectors 
D)  Viral vectors and Enzyme linked immunoglobulin vectors 
E)  All of these

Question 7

The practice of introducing functional gene copies into an individual with two nonfunctional copies is known as:

Accepted Answer

A)  Gene cloning 
B)  Gene therapy 
C)  Gene diagnostics 
D)  Southern blotting 
E)  None of these 
A)  Gene cloning 
B)  Gene therapy 
C)  Gene diagnostics 
D)  Southern blotting 
E)  None of these

Question 8

Which of the following requirements must be fulfilled before a gene therapy procedure will be approved?
1)The gene must be cloned and well characterized
2)The risks of gene therapy to the patient must have been carefully evaluated and shown to be minimum
3)The disease must not be treatable by other strategies

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 9

Which of the following was critical in identifying the CF gene for cystic fibrosis?

Accepted Answer

A)  Use of a sweat gland cDNA library 
B)  Unique structure of the CF gene product 
C)  Important clues from biochemical analysis 
D)  Characteristic symptoms of the disease 
E)  None of these 
A)  Use of a sweat gland cDNA library 
B)  Unique structure of the CF gene product 
C)  Important clues from biochemical analysis 
D)  Characteristic symptoms of the disease 
E)  None of these

Question 10

The gene for which of the following diseases was identified by positional cloning?

Accepted Answer

A)  Huntington's disease 
B)  Cystic fibrosis 
C)  Breast Cancer 
D)  Huntington's disease and Cystic fibrosis 
E)  All of these 
A)  Huntington's disease 
B)  Cystic fibrosis 
C)  Breast Cancer 
D)  Huntington's disease and Cystic fibrosis 
E)  All of these

Question 11

How do antisense mRNA molecules work?

Accepted Answer

A)  Annealing to DNA to prevent replication 
B)  Annealing to DNA to prevent transcription 
C)  Being translated into non-functional protein molecules 
D)  Being translated into functional protein molecules that interact with normal protein molecules 
E)  Annealing to sense mRNA to prevent translation. 
A)  Annealing to DNA to prevent replication 
B)  Annealing to DNA to prevent transcription 
C)  Being translated into non-functional protein molecules 
D)  Being translated into functional protein molecules that interact with normal protein molecules 
E)  Annealing to sense mRNA to prevent translation.

Question 12

What types of viral vectors are commonly used to conduct somatic cell gene therapy? What are the advantages and disadvantages of each?

Accepted Answer

To perform somatic-cell gene therapy,wil

Question 13

The most commonly used method for making transgenic plants is:

Accepted Answer

A)  Electroporation 
B)  Microprojectile bombardment 
C)  A. tumefaciens-mediated transformation 
D)  Retrovirus-mediated genetic transfer. 
E)  Transposon-mediated genetic transfer 
A)  Electroporation 
B)  Microprojectile bombardment 
C)  A. tumefaciens-mediated transformation 
D)  Retrovirus-mediated genetic transfer. 
E)  Transposon-mediated genetic transfer

Question 14

Seventy percent of all Cystic Fibrosis cases are caused by which of the following mutations?

Accepted Answer

A)   8 trinucleotide deletion 
B)   8 trinucleotide repeat 
C)  CAG trinucleotide repeat 
D)  CAG trinuclotide deletion 
E)  None of these 
A)   8 trinucleotide deletion 
B)   8 trinucleotide repeat 
C)  CAG trinucleotide repeat 
D)  CAG trinuclotide deletion 
E)  None of these

Question 15

How does the trinucleotide repeat in the huntingtin gene cause the clinical manifestations of Huntington's Disease?

Accepted Answer

The expanded CAG repeat region in the mu

Question 16

Which of the following human proteins has been produced in microorganisms?

Accepted Answer

A)  Insulin 
B)  Dystrophin 
C)  Apo-dystrophin 
D)  COVERT protein 
E)  Huntingtin gene product 
A)  Insulin 
B)  Dystrophin 
C)  Apo-dystrophin 
D)  COVERT protein 
E)  Huntingtin gene product

Question 17

Why might a patient with ADA-SCID be a good candidate for somatic cell gene therapy?
1)The ADA gene was one of the first human disease genes to be cloned and characterized.
2)White blood cells can easily be obtained from ADA - SCID patients and reintroduced after functional copies of the ADA gene are added
3)Even a small amount of functional ADA will restore partial immune function

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 18

Which of the following statements regarding paternity testing is correct?

Accepted Answer

A)  Blood type data can be used to positively identify the father of a child 
B)  Blood type data has several advantages over DNA fingerprinting in paternity testing 
C)  For paternity testing, only DNA samples from the possible fathers and the child are required. 
D)  Approximately half of the bands in the child's DNA print match those of the father. 
E)  Increasing the number of hybridizing probes increases the ambiguity of the paternity test. 
A)  Blood type data can be used to positively identify the father of a child 
B)  Blood type data has several advantages over DNA fingerprinting in paternity testing 
C)  For paternity testing, only DNA samples from the possible fathers and the child are required. 
D)  Approximately half of the bands in the child's DNA print match those of the father. 
E)  Increasing the number of hybridizing probes increases the ambiguity of the paternity test.

Question 19

Which of the following statements true regarding germ line cell gene therapy is false?
1)It is currently being practiced on humans.
2)There are no moral or ethical considerations with this type of gene therapy
3)It stops the diseased gene from being passed down to offspring

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 20

Which of the following was the first pharmaceutical protein produced by bacteria approved for human use?

Accepted Answer

A)  Insulin 
B)  hGH 
C)  Erythropoietin 
D)  Rennin 
E)  Interferon 
A)  Insulin 
B)  hGH 
C)  Erythropoietin 
D)  Rennin 
E)  Interferon

Exam 16: Applications Of Molecular Genetics

Which of the following statements regarding DNA fingerprinting is true

How is hGH produced for pharmaceutical use by using E.coli as a producer?

Which of the following can be attributed to the presence of unstable trinucleotide repeats?

When confirming paternity it would be best to use: 1)Multiple numbers of probes so that the genome can be categorized more accurately 2)Multiple potential father's DNA samples so as to rule out infidelity 3)Unknown samples of DNA to prevent bias

Which of the following is used to make cheese?

Which of the following is a common method of delivery used for somatic cell gene therapy?

The practice of introducing functional gene copies into an individual with two nonfunctional copies is known as:

Which of the following was critical in identifying the CF gene for cystic fibrosis?

The gene for which of the following diseases was identified by positional cloning?

How do antisense mRNA molecules work?

What types of viral vectors are commonly used to conduct somatic cell gene therapy? What are the advantages and disadvantages of each?

The most commonly used method for making transgenic plants is:

Seventy percent of all Cystic Fibrosis cases are caused by which of the following mutations?

How does the trinucleotide repeat in the huntingtin gene cause the clinical manifestations of Huntington's Disease?

Which of the following human proteins has been produced in microorganisms?

Which of the following statements regarding paternity testing is correct?

Which of the following statements true regarding germ line cell gene therapy is false? 1)It is currently being practiced on humans. 2)There are no moral or ethical considerations with this type of gene therapy 3)It stops the diseased gene from being passed down to offspring

Which of the following was the first pharmaceutical protein produced by bacteria approved for human use?

The Science Of Genetics

Cellular Reproduction

Mendelism The Basic Principles Of Inheritance

Extensions Of Mendelism

The Chromosomal Basis Of Mendelism

Variation In Chromosome Number And Structure

Linkage,Crossing Over,And Chromosome Mapping In …

The Genetics Of Bacteria And Their Viruses

DNA And The Molecular Structure Of Chromosomes

Replication Of DNA And Chromosomes

Transcription And RNA Processing

Translation And The Genetic Code

Mutation,DNA Repair,And Recombination

The Techniques Of Molecular Genetics

Genomics

Transposable Genetics Elements

Regulation Of Gene Expression In Prokaryotes

Regulation Of Gene Expression In Eukaryotes

The Genetic Control Of Animal Development

The Genetic Basis Of Cancer

Inheritance Of Complex Traits

Population Genetics

Evolutionary Genetics

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