Exam 8: Molecular Biology and Transcription and Rna Processing

Spliceosome components are recruited to 3′ and 5′ splice sites by SR proteins bound to what DNA sequences?

What proteins are bound to enhancers, forming a protein "bridge" that bends the DNA over the promoter to initiate transcription?

You wish to create a mutation in which you prevent access of RNA polymerase to the gene. Which region of a gene would you target?

Which assay allows you to identify the exact location of the protein-binding sequence within a promoter?

The rat α-tropomyosin (α-Tm)gene produces nine different mature mRNA proteins from a single gene using which three "alternative" mechanisms?

A plant cell exhibits defects in transcription of transfer RNA genes. The gene encoding which polymerase is likely to be mutated in this cell?

Which of the following is part of a DNA molecule?

RNA polymerase is called a ________, meaning it is an intact complex with full enzymatic capacity.

What are the three types of posttranscriptional processing, and what are the consequences of preventing each of these modifications in terms of gene expression?

What molecule is required for activation of a rho protein?

Which enzyme is required to initiate 5′ capping of eukaryotic mRNA transcripts by removing the terminal phosphate group?

Intron self-splicing occurs when two ________ reactions excise the intron and allow exons to ligate.

If bacteria are infected with a bacteriophage and briefly exposed to radioactive uracil, what results would you expect to see immediately after exposure?

In intrinsic termination, inverted repeat DNA sequences followed immediately by a string of ________ produce an mRNA stem-loop, followed by a string of ________.

A cell exhibits specific defects in the alternative intron splicing pathway. The genes encoding which proteins are likely mutated in this cell?

One type of RNA editing involves inserting uracils into edited mRNA with the assistance of which type of specialized RNA?

In the following prokaryotic DNA sequence, label the highlighted regions. Using the prokaryotic DNA sequence above, describe the relationship between the highlighted regions and how mutations in each region may affect gene expression.

The 5S rRNA and tRNA genes have internal promoters, located downstream of the transcription start site, called ________.

Following a DNA footprint protection assay, which banding pattern would you expect to see if you identify a potential promoter region?

What is the -35 consensus sequence for the following sequences?