Exam 9: DNA structure and Analysis

Is the accompanying figure DNA or RNA? Is the circle closer to the 5' or 3' end?

Consider the structure of double-stranded DNA.When DNA is placed into distilled water,it denatures; however,by adding NaCl,the DNA renatures.Why?

DNA has no sulfur,and proteins have no phosphorus.

Describe four major functions of DNA in a cell.

What was the Avery et al.'s (1944)contribution to the understanding of molecular biology?

All other factors being equal,the renaturation of the three classes of complementary nucleic acid sequences occurs in what order,from fastest to slowest?

If the GC content of a DNA molecule is 60%,what are the molar percentages of the four bases (G,C,T,A)?

During the polymerization of nucleic acids,covalent bonds are formed between neighboring nucleotides.Which carbons are involved in such bonds?

Experiments conducted in the 1920s by Frederick Griffith involving the bacterium Diplococcus pneumoniae demonstrated that a substance from one bacterial strain could genetically transform other bacterial strains.What was the name of the substance capable of such transformation,and who finally determined its identity?

The strongest direct evidence that DNA is the genetic material comes from which contemporary methodology?

What are the two major components of the Tobacco Mosaic Virus?

Regarding the structure of DNA,the covalently arranged combination of a deoxyribose and a nitrogenous base would be called a(n)________.

Is the accompanying figure DNA or RNA? ________ Is the arrow closest to the 5' or 3' end? ________ Spleen diesterase is an enzyme that breaks the covalent bond that connects the phosphate to the 5' carbon.Assume that the dinucleotide is digested with spleen diesterase.To which base and to which carbon on the sugar is the phosphate now attached,A or T?

The basic structure of a nucleotide includes ________.

Briefly define transformation and describe the relationship between the phenomenon of transformation and the discovery that DNA is the genetic material in bacteria.

At what approximate wavelengths do DNA,RNA,and proteins maximally absorb light?

Hershey and Chase used labeled DNA and protein to determine that DNA is the genetic material in bacteria.

In the classic experiment conducted by Hershey and Chase,why was the pellet radioactive in the centrifuge tube that contained bacteria with viruses?

When and in which journal did Watson and Crick publish their now-famous paper entitled "Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid."

(a)Assume that A + T/G + C equals 0.5 in one strand of DNA.What is the ratio of these bases in the complementary strand? (b)If A + G/T + C equals 0.5 in one strand,what is the ratio of these bases in the complementary strand?