Question 1

In a typical PCR,primers are used to cleave specific regions of the DNA template.

Accepted Answer

A) True 
 B)False  False

Question 2

Molecular biologists rely on many,often sophisticated,techniques to pursue their discipline.Ultracentrifugation,electron microscopy,X-ray diffraction,electrophoresis,and computer interfacing are fundamental tools.Model organisms provide the raw materials for study.List three organisms (or organismic groups)often used by recombinant DNA technologists and describe a major advantage of each group.

Accepted Answer

Bacteriophage: useful vector,relatively simple genome,short generation time.Bacteria: relatively simple,short generation time,simple growth requirements,well understood genetics,transformable.Viruses: capable of carrying recombinant DNA and infecting eukaryotic cells.Yeast: relatively simple for a eukaryote,short generation time,simple growth requirements,transformable.

Question 3

If a researcher wishes to clone a gene using typical restriction endonucleases,how does the restriction endonuclease recognize genes in the genome?

Accepted Answer

Restriction endonucleases do not recognize functional regions in the genome (genes).They can recognize only relatively short DNA sequences,which have no relationship to functionality.

Question 4

Assume that you have cut λ DNA with the restriction enzyme HindIII.You separate the fragments on an agarose gel and stain the DNA with ethidium bromide.You notice that the intensity of the stain is less in the bands that have migrated closer to the "+" pole.Give an explanation for this finding.

Accepted Answer

Because the smaller fragments

Question 5

In a PCR,primers are complementary to stretches of DNA with which they anneal.

Accepted Answer

A) True 
 B)False

Question 6

Nucleic acid blotting is widely used in recombinant DNA technology.In a Southern blot,one generally ________.

Accepted Answer

A) hybridizes filter-bound DNA with a DNA probe 
B) hybridizes filter-bound RNA with a DNA probe 
C) examines amino acid substitutions with radioactive probes 
D) cleaves RNA with restriction endonucleases 
E) ligates DNA with DNA ligase 
A) hybridizes filter-bound DNA with a DNA probe 
B) hybridizes filter-bound RNA with a DNA probe 
C) examines amino acid substitutions with radioactive probes 
D) cleaves RNA with restriction endonucleases 
E) ligates DNA with DNA ligase

Question 7

A restriction map provides the location of sites cleaved by restriction enzymes.

Accepted Answer

A) True 
 B)False

Question 8

In what way are specific DNA sequences of the template amplified in the polymerase chain reaction? In other words,how does one target the target?

Accepted Answer

Oligonucleotide primers associ

Question 9

Some restriction enzymes cleave DNA in such a manner as to produce blunt ends.Ligation of blunt end fragments is most often enhanced by the use of the enzyme terminal deoxynucleotidyl transferase.Speculate on the function of deoxynucleotidyl transferase in terms of using blunt end fragments in cloning.

Accepted Answer

Terminal deoxynucleotidyl transferase ex

Question 10

Words such as did,mom,and pop have something in common with the fundamental tool of recombinant DNA technology.In the context of recombinant DNA technology,which term would be used to describe such words?

Accepted Answer

A) lysogenic 
B) prototrophic 
C) palindromic 
D) conjugation 
E) insertion 
A) lysogenic 
B) prototrophic 
C) palindromic 
D) conjugation 
E) insertion

Question 11

When propagating a clone in the lambda phage,would you have more immediate success if the phage entered the lysogenic cycle or the lytic cycle?

Accepted Answer

The answer of When propagating a clone in the lambda...

Question 12

Assume that a researcher conducted a cloning experiment using a typical plasmid,transformed an appropriate host bacterial strain,and plated the bacteria on an appropriate X-gal medium.Blue and white colonies appeared.Which of the two types of colonies,blue or white,would more likely contain the recombinant plasmid? Why?

Accepted Answer

the white colonies b

Question 13

What is the specific application of reverse transcriptase in the preparation of cDNA?

Accepted Answer

synthesis

Question 14

Under ideal conditions,how many copies of all the sequences of the host genome should be represented in a genomic library?

Accepted Answer

At least one should be represented.Typic

Question 15

The main purpose of a probe is its insertion in plasmid DNA.

Accepted Answer

A) True 
 B)False

Question 16

E.coli is a common YAC.

Accepted Answer

A) True 
 B)False

Question 17

What might be a reasonable function of restriction endonucleases in a bacterium,distinct from their use by molecular biologists?

Accepted Answer

Isolated from bacteria,restric

Question 18

In the polymerase chain reaction,what is the purpose of the initial high temperature? What is the purpose of cooling in the second step?

Accepted Answer

to denature the targ

Question 19

A common term for a plasmid or other DNA element that serves as a cloning vehicle is vector.

Accepted Answer

A) True 
 B)False

Question 20

During a PCR,heat is provided to inactivate the polymerase enzyme.

Accepted Answer

A) True 
 B)False

In a typical PCR,primers are used to cleave specific regions of the DNA template.

If a researcher wishes to clone a gene using typical restriction endonucleases,how does the restriction endonuclease recognize genes in the genome?

Assume that you have cut λ DNA with the restriction enzyme HindIII.You separate the fragments on an agarose gel and stain the DNA with ethidium bromide.You notice that the intensity of the stain is less in the bands that have migrated closer to the "+" pole.Give an explanation for this finding.

In a PCR,primers are complementary to stretches of DNA with which they anneal.

Nucleic acid blotting is widely used in recombinant DNA technology.In a Southern blot,one generally ________.

A restriction map provides the location of sites cleaved by restriction enzymes.

In what way are specific DNA sequences of the template amplified in the polymerase chain reaction? In other words,how does one target the target?

Words such as did,mom,and pop have something in common with the fundamental tool of recombinant DNA technology.In the context of recombinant DNA technology,which term would be used to describe such words?

When propagating a clone in the lambda phage,would you have more immediate success if the phage entered the lysogenic cycle or the lytic cycle?

What is the specific application of reverse transcriptase in the preparation of cDNA?

Under ideal conditions,how many copies of all the sequences of the host genome should be represented in a genomic library?

The main purpose of a probe is its insertion in plasmid DNA.

E.coli is a common YAC.

What might be a reasonable function of restriction endonucleases in a bacterium,distinct from their use by molecular biologists?

In the polymerase chain reaction,what is the purpose of the initial high temperature? What is the purpose of cooling in the second step?

A common term for a plasmid or other DNA element that serves as a cloning vehicle is vector.

During a PCR,heat is provided to inactivate the polymerase enzyme.

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Modification of Mendelian Ratios

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Linkage and Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

DNA structure and Analysis

DNA Replication and Recombination

Chromosome Structure and DNA Sequence Organization

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation,DNA Repair,and Transposition

Regulation of Gene Expression

The Genetics of Cancer

Genomics,Bioinformatics,and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Developmental Genetics

Quantitative Genetics and Multifactorial Traits

Population and Evolutionary Genetics

Conservation Genetics

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Exam 17: Recombinant DNA Technology

In a typical PCR,primers are used to cleave specific regions of the DNA template.

If a researcher wishes to clone a gene using typical restriction endonucleases,how does the restriction endonuclease recognize genes in the genome?

Assume that you have cut λ DNA with the restriction enzyme HindIII.You separate the fragments on an agarose gel and stain the DNA with ethidium bromide.You notice that the intensity of the stain is less in the bands that have migrated closer to the "+" pole.Give an explanation for this finding.

In a PCR,primers are complementary to stretches of DNA with which they anneal.

Nucleic acid blotting is widely used in recombinant DNA technology.In a Southern blot,one generally ________.

A restriction map provides the location of sites cleaved by restriction enzymes.

In what way are specific DNA sequences of the template amplified in the polymerase chain reaction? In other words,how does one target the target?

Words such as did,mom,and pop have something in common with the fundamental tool of recombinant DNA technology.In the context of recombinant DNA technology,which term would be used to describe such words?

When propagating a clone in the lambda phage,would you have more immediate success if the phage entered the lysogenic cycle or the lytic cycle?

What is the specific application of reverse transcriptase in the preparation of cDNA?

Under ideal conditions,how many copies of all the sequences of the host genome should be represented in a genomic library?

The main purpose of a probe is its insertion in plasmid DNA.

E.coli is a common YAC.

What might be a reasonable function of restriction endonucleases in a bacterium,distinct from their use by molecular biologists?

In the polymerase chain reaction,what is the purpose of the initial high temperature? What is the purpose of cooling in the second step?

A common term for a plasmid or other DNA element that serves as a cloning vehicle is vector.

During a PCR,heat is provided to inactivate the polymerase enzyme.

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Modification of Mendelian Ratios

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Linkage and Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

DNA structure and Analysis

DNA Replication and Recombination

Chromosome Structure and DNA Sequence Organization

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation,DNA Repair,and Transposition

Regulation of Gene Expression

The Genetics of Cancer

Genomics,Bioinformatics,and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Developmental Genetics

Quantitative Genetics and Multifactorial Traits

Population and Evolutionary Genetics

Conservation Genetics

Filters