Question 1

Write an explanation for why a bacteriophage, a virus that infects bacterial cells, would be a good cloning vector.

Accepted Answer

As a virus, a bacteriophage has a piece

Question 2

DNA fragments cut by most restriction enzymes have:

Accepted Answer

A)  double-stranded complementary ends. 
B)  either only sequences of Gs or only sequences of Cs. 
C)  cuts made at random points along one of the strands. 
D)  protruding sticky ends. 
A)  double-stranded complementary ends. 
B)  either only sequences of Gs or only sequences of Cs. 
C)  cuts made at random points along one of the strands. 
D)  protruding sticky ends.

Question 3

Much of the food crops in the United States are transgenic. This primarily has been done to give the crops what properties?

Accepted Answer

A)  a decrease in the need for chemical fertilizers 
B)  the ability to produce human growth hormone 
C)  the ability to resist insects and herbicides 
D)  the need for greater irrigation 
A)  a decrease in the need for chemical fertilizers 
B)  the ability to produce human growth hormone 
C)  the ability to resist insects and herbicides 
D)  the need for greater irrigation

Question 4

Stem cells are said to be pluripotent. This means they have the ability to:

Accepted Answer

A)  form blastocysts. 
B)  be reprogrammed through recombinant DNA technology. 
C)  make more of themselves in culture. 
D)  specialize into any other type of cell. 
E)  specialize into only a few different types of cells such as nerves and muscle cells. 
A)  form blastocysts. 
B)  be reprogrammed through recombinant DNA technology. 
C)  make more of themselves in culture. 
D)  specialize into any other type of cell. 
E)  specialize into only a few different types of cells such as nerves and muscle cells.

Question 5

If you compared two samples of DNA at three different locations in the genome and found they had different lengths of short tandem repeats at each location, what could you conclude?

Accepted Answer

A)  The two samples came from different individuals. 
B)  The two samples came from the same individual. 
C)  The two samples came from females. 
D)  The two samples came from males. 
A)  The two samples came from different individuals. 
B)  The two samples came from the same individual. 
C)  The two samples came from females. 
D)  The two samples came from males.

Question 6

Genetically modified crops have been produced mainly to benefit:

Accepted Answer

A)  consumers. 
B)  farmers. 
C)  the scientific community. 
D)  the United State's ability to export agricultural products. 
A)  consumers. 
B)  farmers. 
C)  the scientific community. 
D)  the United State's ability to export agricultural products.

Question 7

What is a clone?

Accepted Answer

A)  any cell that contains recombinant DNA 
B)  a protein produced by a recombinant bacterium 
C)  an exact copy of a DNA, cell, or organism 
D)  any animal that contains recombinant DNA 
A)  any cell that contains recombinant DNA 
B)  a protein produced by a recombinant bacterium 
C)  an exact copy of a DNA, cell, or organism 
D)  any animal that contains recombinant DNA

Question 8

Why is forensic DNA analysis an important part of biotechnology?

Accepted Answer

A)  It is used to amplify small samples of DNA. 
B)  It is used to match two samples of DNA. 
C)  It is used to produce transgenic organisms. 
D)  It substitutes for restriction enzymes. 
A)  It is used to amplify small samples of DNA. 
B)  It is used to match two samples of DNA. 
C)  It is used to produce transgenic organisms. 
D)  It substitutes for restriction enzymes.

Question 9

One of the main problems with growing Bt cotton and other Bt-enhanced crops is the Bt toxin:

Accepted Answer

A)  can produce populations of insects resistant to it. 
B)  can create Bt refuges near the fields that harbor bugs not resistant to Bt. 
C)  reduces the need for insecticides. 
D)  can be harmful to humans. 
A)  can produce populations of insects resistant to it. 
B)  can create Bt refuges near the fields that harbor bugs not resistant to Bt. 
C)  reduces the need for insecticides. 
D)  can be harmful to humans.

Question 10

These days many of our food crops are genetically modified. To some people this "frankenfood" poses many dangers and should not be used. Do you agree? Why or why not?

Accepted Answer

Arguments that the genetically modified

Question 11

The enzyme EcoRI cuts between the G and the A in the sequence GAATTC. The enzyme BamHI cuts between adjacent G nucleotides in the sequence GGATCC. The sticky ends that are generated by the two enzymes are complementary and will stick together.

Accepted Answer

A) True 
 B)False

Question 12

You are a crime scene investigator, and the only evidence you are able to gather at the crime scene are some strands of hair. You want to see whether a suspect in custody was at the crime scene. What technique would you use to try and connect the suspect to the crime scene?

Accepted Answer

A)  PCR followed by cloning 
B)  cloning and transgenics 
C)  PCR followed by analyzing small tandem repeats 
D)  recombinant DNA techniques 
A)  PCR followed by cloning 
B)  cloning and transgenics 
C)  PCR followed by analyzing small tandem repeats 
D)  recombinant DNA techniques

Question 13

The combination of a somatic cell with an enucleated egg cell is:

Accepted Answer

A)  transformation. 
B)  cell reprogramming. 
C)  somatic cell nuclear transfer. 
D)  transgenic biotechnology. 
A)  transformation. 
B)  cell reprogramming. 
C)  somatic cell nuclear transfer. 
D)  transgenic biotechnology.

Question 14

One advantage of using bacteria in recombinant DNA technology is that they have a ring of DNA that is not part of their chromosome called a:

Accepted Answer

A)  plasmid. 
B)  virus. 
C)  bacteriophage. 
D)  restriction fragment. 
A)  plasmid. 
B)  virus. 
C)  bacteriophage. 
D)  restriction fragment.

Question 15

How might stem cells be used to treat disease?

Accepted Answer

When inserted into a human suffering fro

Question 16

What is the advantage of implanting iPS cells derived from the patient's own body rather than ESCs?

Accepted Answer

A)  iPS cells can differentiate into the needed cell types, but ESCs cannot. 
B)  ESCs could be rejected by the patient's body because they are seen as foreign. 
C)  ESCs have the potential to migrate to the wrong part of the body, but iPS cells will not. 
D)  ESCs have HLA markers, but iPS cells from the patient do not. 
A)  iPS cells can differentiate into the needed cell types, but ESCs cannot. 
B)  ESCs could be rejected by the patient's body because they are seen as foreign. 
C)  ESCs have the potential to migrate to the wrong part of the body, but iPS cells will not. 
D)  ESCs have HLA markers, but iPS cells from the patient do not.

Question 17

Reproductive cloning has been used to clone humans.

Accepted Answer

A) True 
 B)False

Question 18

What property of restriction enzymes allows DNA from different organisms to be combined?

Accepted Answer

A)  Restriction enzymes cut DNA randomly, so any DNAs might be combined. 
B)  Restriction enzymes cut only small transformable fragments, not large chromosomal DNA. 
C)  Because restriction enzymes are naturally found in bacteria, they cut all transformed DNA. 
D)  If the same restriction enzyme cuts two different DNAs, the ends are complementary. 
A)  Restriction enzymes cut DNA randomly, so any DNAs might be combined. 
B)  Restriction enzymes cut only small transformable fragments, not large chromosomal DNA. 
C)  Because restriction enzymes are naturally found in bacteria, they cut all transformed DNA. 
D)  If the same restriction enzyme cuts two different DNAs, the ends are complementary.

Question 19

"Golden rice" is a transgenic form of rice because it:

Accepted Answer

A)  produces omega-3 fatty acids. 
B)  contains genes from a bacterium and a daffodil. 
C)  produces insulin. 
D)  produces cancer-fighting drugs. 
A)  produces omega-3 fatty acids. 
B)  contains genes from a bacterium and a daffodil. 
C)  produces insulin. 
D)  produces cancer-fighting drugs.

Question 20

Restriction enzymes are mainly derived from:

Accepted Answer

A)  yeast. 
B)  fungi. 
C)  bacteria. 
D)  animal cells. 
A)  yeast. 
B)  fungi. 
C)  bacteria. 
D)  animal cells.

Exam 15: The Future Isnt What It Used to Be: Biotechnology

Write an explanation for why a bacteriophage, a virus that infects bacterial cells, would be a good cloning vector.

DNA fragments cut by most restriction enzymes have:

Much of the food crops in the United States are transgenic. This primarily has been done to give the crops what properties?

Stem cells are said to be pluripotent. This means they have the ability to:

If you compared two samples of DNA at three different locations in the genome and found they had different lengths of short tandem repeats at each location, what could you conclude?

Genetically modified crops have been produced mainly to benefit:

What is a clone?

Why is forensic DNA analysis an important part of biotechnology?

One of the main problems with growing Bt cotton and other Bt-enhanced crops is the Bt toxin:

These days many of our food crops are genetically modified. To some people this "frankenfood" poses many dangers and should not be used. Do you agree? Why or why not?

The enzyme EcoRI cuts between the G and the A in the sequence GAATTC. The enzyme BamHI cuts between adjacent G nucleotides in the sequence GGATCC. The sticky ends that are generated by the two enzymes are complementary and will stick together.

You are a crime scene investigator, and the only evidence you are able to gather at the crime scene are some strands of hair. You want to see whether a suspect in custody was at the crime scene. What technique would you use to try and connect the suspect to the crime scene?

The combination of a somatic cell with an enucleated egg cell is:

One advantage of using bacteria in recombinant DNA technology is that they have a ring of DNA that is not part of their chromosome called a:

How might stem cells be used to treat disease?

What is the advantage of implanting iPS cells derived from the patient's own body rather than ESCs?

Reproductive cloning has been used to clone humans.

What property of restriction enzymes allows DNA from different organisms to be combined?

"Golden rice" is a transgenic form of rice because it:

Restriction enzymes are mainly derived from:

Science As a Way of Learning: a Guide to the Natural World

Fundamental Building Blocks: Chemistry, Water, and Ph

Lifes Components: Biological Molecules

Lifes Home: the Cell

Lifes Border: the Plasma Membrane

Lifes Mainspring: an Introduction to Energy

Vital Harvest: Deriving Energy From Food

The Green Worlds Gift: Photosynthesis

The Links in Lifes Chain: Genetics and Cell Division

Preparing for Sexual Reproduction: Meiosis

The First Geneticist: Mendel and His Discoveries

Units of Heredity: Chromosomes and Inheritance

Passing on Lifes Information: Dna Structure and Replication

How Proteins Are Made: Genetic Transcription, Translation, and Regulation

An Introduction to Evolution: Charles Darwin, Evolutionary Thought, and the Evidence for Evolution

The Means of Evolution: Microevolution

The Outcomes of Evolution: Macroevolution

A Slow Unfolding: the History of Life on Earth

Arriving Late, Traveling Far: the Evolution of Human Beings

Viruses, Bacteria, Archaea, and Protists: the Diversity of Life 1

Fungi : the Diversity of Life 2

Animals: the Diversity of Life 3

Plants: the Diversity of Life 4

The Angiosperms: Form and Function in Flowering Plants

Body Support and Movement: the Integumentary, Skeletal, and Muscular Systems

Communication and Control 1: the Nervous System

Communication and Control 2: the Endocrine System

Defending the Body: the Immune System

Transport and Exchange 1: Blood and Breath

Transport and Exchange 2: Digestion, Nutrition, and Elimination

An Amazingly Detailed Script: Animal Development

How the Baby Came to Be: Human Reproduction

An Interactive Living World 1: Populations in Ecology

An Interactive Living World 2: Communities in Ecology

An Interactive Living World 3: Ecosystems and Biomes

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