Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

The RN understands that along with a pH test, a microscopic view is needed to perform the ferning test to detect an amniotic fluid leak. The patient sample is prepared on a glass slide and examined under 10x magnification. Which type of microscope will be used to make this observation of the patient sample?

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the ________ lens. This image is then projected to the ________ lens that will further magnify the specimen to form a virtual image received by the eye.

A pure culture contains ________.

The patient is diagnosed with a bacterial infection after gram-positive cocci are detected in the fluid sample. The Gram stain involves ________.

Which of the following is essential for development of discrete, isolated colonies?

The diameter of field for a 4x lens is measured at 4.6 mm. How many bacterial cells, each measuring 4 μm, could be lined up along the diameter?

For which bacterial genus does mannitol salt agar differentiate between species?

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the ________.

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ________.

Agar is a complex polysaccharide that comes from a(n) ________.

What do the Gram stain, acid-fast stain, and endospore stain have in common?

A confocal scanning microscope ________.

Which of the following controls the amount of light entering the specimen?

Differential media results in which of the following growth characteristics?

The procedures for culturing a microorganism require the use of a microscope.

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue. All the cells appear blue under the oil lens. This is an example of ________.

All of the following are diameters of cells that would be resolved in a microscope with a 0.2 µm limit of resolution except ________.

A rod-shaped bacterium is measured as 0.3 micrometers (μm) in length using an ocular micrometer. Your instructor wants you to report the length in millimeters (mm) to test your understanding of metric conversions. What is the length of the organism in millimeters?

The real image is the reverse of the actual specimen.

Mixed cultures are also referred to as contaminated cultures.