Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

Which of the following is not an inoculating tool?

Some microbes are not capable of growing on artificial media.

Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled, and cooled?

One colony typically develops from the growth of several parent bacterial cells.

The patient's culture results positive for Staphylococcus. The RN understands that the culture most likely required growth on a complex medium, consisting of

All of the following pertain to the fluorescence microscope except ________.

Newly inoculated cultures must be ________ at a specific temperature to encourage growth.

The sterile vaginal fluid specimen is sent for culture. The RN educates the patient about the five basic techniques utilized by laboratory technicians to manipulate, grow, examine, and characterize any microorganisms present in the specimen. Which of the following is the correct order of steps for processing the specimen?

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

The purpose of staining cells on a microscope slide is to ________.

Which type of microscope shows cells against a bright background but also differentiates intracellular structures of unstained cells based on their varying densities?

Comparing defined vs. complex media is analogous to comparing ________.

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

A sputum sample is ordered for microbial analysis in order to rule out the diagnosis of tuberculosis. Suspecting Mycobacterium tuberculosis may be the pathogen, the RN knows the laboratory technicians will perform which stain on the sample?

A microbiologist must culture a patient's feces for intestinal pathogens. Which of the following would likely be present in selective media for analyzing this fecal specimen?

Which of the following is not a benefit of agar as a solid medium?

The ________ of the microscope holds and allows selection of the objective lenses.

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Normal incubation temperatures range from 30°C to 60°C.

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.