Question 1

At the end of the Gram stain, gram-positive bacteria will be seen as red/pink cells.

Accepted Answer

A) True 
 B)False

Question 2

The Five I's of studying microorganisms include all of the following except ________.

Accepted Answer

A)  inoculation 
B)  incubation 
C)  infection 
D)  isolation 
E)  identification 
A)  inoculation 
B)  incubation 
C)  infection 
D)  isolation 
E)  identification

Question 3

A nutrient medium that contains at least one ingredient that is NOT chemically definable would be termed ________.

Accepted Answer

A)  complex 
B)  reducing 
C)  enriched 
D)  synthetic 
E)  minimal 
A)  complex 
B)  reducing 
C)  enriched 
D)  synthetic 
E)  minimal

Question 4

A confocal microscope uses visible light as its source of illumination.

Accepted Answer

A) True 
 B)False

Question 5

Which of the following magnifies the specimen to produce its real image?

Accepted Answer

A)  Condenser 
B)  Objective lens 
C)  Ocular lens 
D)  Body 
E)  Nosepiece 
A)  Condenser 
B)  Objective lens 
C)  Ocular lens 
D)  Body 
E)  Nosepiece

Question 6

When utilizing a light microscope, the specimen on the glass slide must be in proper position to ensure illumination of the specimen for visualization. The glass slide is placed in which of the following positions?

Accepted Answer

A)  Between the condenser lens and the objective lens 
B)  Directly on top of the light source 
C)  Between the ocular lens and the objective lens 
D)  Between the light source and the condenser lens 
A)  Between the condenser lens and the objective lens 
B)  Directly on top of the light source 
C)  Between the ocular lens and the objective lens 
D)  Between the light source and the condenser lens

Question 7

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?

Accepted Answer

A)  The microbiologist used too much inoculum. 
B)  The culture is contaminated. 
C)  The incubation temperature was incorrect. 
D)  The culture medium must be selective. 
E)  The culture medium must be differential. 
A)  The microbiologist used too much inoculum. 
B)  The culture is contaminated. 
C)  The incubation temperature was incorrect. 
D)  The culture medium must be selective. 
E)  The culture medium must be differential.

Question 8

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

Accepted Answer

A)  Transport 
B)  EMB 
C)  Blood 
D)  Thioglycollate 
E)  General purpose 
A)  Transport 
B)  EMB 
C)  Blood 
D)  Thioglycollate 
E)  General purpose

Question 9

Which of the following magnifies the specimen to produce its virtual image?

Accepted Answer

A)  Objective lens 
B)  Ocular lens 
C)  Condenser 
D)  Body 
E)  Iris diaphragm 
A)  Objective lens 
B)  Ocular lens 
C)  Condenser 
D)  Body 
E)  Iris diaphragm

Question 10

The relationship between a micrometer and a millimeter is ________.

Accepted Answer

A)  a millimeter is 10 times larger than a micrometer 
B)  a micrometer is 1,000 times smaller than a millimeter 
C)  a millimeter is 100 times smaller than a micrometer 
D)  a micrometer is 1,000 times larger than a millimeter 
E)  a millimeter is 10 times smaller than a micrometer 
A)  a millimeter is 10 times larger than a micrometer 
B)  a micrometer is 1,000 times smaller than a millimeter 
C)  a millimeter is 100 times smaller than a micrometer 
D)  a micrometer is 1,000 times larger than a millimeter 
E)  a millimeter is 10 times smaller than a micrometer

Question 11

An enveloped virus measures 0.02 micrometers (μm) in diameter. What is the diameter of this virus in nanometers (nm)?

Accepted Answer

A)  20 nm 
B)  0.00002 nm 
C)  2 nm 
D)  0.2 nm 
A)  20 nm 
B)  0.00002 nm 
C)  2 nm 
D)  0.2 nm

Question 12

Basic dyes are ________.

Accepted Answer

A)  attracted to the negatively charged acidic substances of bacterial cells 
B)  anionic 
C)  used in negative staining 
D)  repelled by cells 
E)  dyes such as India ink and nigrosin 
A)  attracted to the negatively charged acidic substances of bacterial cells 
B)  anionic 
C)  used in negative staining 
D)  repelled by cells 
E)  dyes such as India ink and nigrosin

Question 13

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 14

In the absence of immersion oil when using the 100x objective lens, some light would be lost to scatter resulting in a blurry image.

Accepted Answer

A) True 
 B)False

Question 15

A reducing medium contains ________.

Accepted Answer

A)  sugars that can be fermented 
B)  extra oxygen 
C)  hemoglobin, vitamins, or other growth factors 
D)  substances that remove oxygen 
E)  inhibiting agents 
A)  sugars that can be fermented 
B)  extra oxygen 
C)  hemoglobin, vitamins, or other growth factors 
D)  substances that remove oxygen 
E)  inhibiting agents

Question 16

Which type of microscope is the most widely used and shows cells against a bright background?

Accepted Answer

A)  Bright field 
B)  Dark field 
C)  Phase contrast 
D)  Fluorescence 
E)  Electron 
A)  Bright field 
B)  Dark field 
C)  Phase contrast 
D)  Fluorescence 
E)  Electron

Question 17

Bacterial cultures are easily identified from their microscopic appearance.

Accepted Answer

A) True 
 B)False

Question 18

Which type of microscope does not use light in forming the specimen image?

Accepted Answer

A)  Bright field 
B)  Dark field 
C)  Phase contrast 
D)  Fluorescence 
E)  Electron 
A)  Bright field 
B)  Dark field 
C)  Phase contrast 
D)  Fluorescence 
E)  Electron

Question 19

Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?

Accepted Answer

A)  Resolving power 
B)  Magnification 
C)  Refraction 
D)  All of the choices are correct. 
E)  None of the choices are correct. 
A)  Resolving power 
B)  Magnification 
C)  Refraction 
D)  All of the choices are correct. 
E)  None of the choices are correct.

Question 20

A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture. What is the most likely explanation?

Accepted Answer

A)  The microbiologist used too much inoculum. 
B)  The culture is contaminated. 
C)  The incubation temperature was incorrect. 
D)  The culture medium must be selective. 
E)  The culture medium must be differential. 
A)  The microbiologist used too much inoculum. 
B)  The culture is contaminated. 
C)  The incubation temperature was incorrect. 
D)  The culture medium must be selective. 
E)  The culture medium must be differential.

Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

At the end of the Gram stain, gram-positive bacteria will be seen as red/pink cells.

The Five I's of studying microorganisms include all of the following except ________.

A nutrient medium that contains at least one ingredient that is NOT chemically definable would be termed ________.

A confocal microscope uses visible light as its source of illumination.

Which of the following magnifies the specimen to produce its real image?

When utilizing a light microscope, the specimen on the glass slide must be in proper position to ensure illumination of the specimen for visualization. The glass slide is placed in which of the following positions?

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

Which of the following magnifies the specimen to produce its virtual image?

The relationship between a micrometer and a millimeter is ________.

An enveloped virus measures 0.02 micrometers (μm) in diameter. What is the diameter of this virus in nanometers (nm)?

Basic dyes are ________.

The bending of light rays as they pass from one medium to another is called refraction.

In the absence of immersion oil when using the 100x objective lens, some light would be lost to scatter resulting in a blurry image.

A reducing medium contains ________.

Which type of microscope is the most widely used and shows cells against a bright background?

Bacterial cultures are easily identified from their microscopic appearance.

Which type of microscope does not use light in forming the specimen image?

Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?

A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture. What is the most likely explanation?

Introduction to Microbes and Their Building Blocks

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

Viral Structure and Multiplication

Microbial Nutrition and Growth

Microbial Metabolism

Microbial Genetics and Genetic Engineering

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Interactions Between Microbes and Humans

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses Ii: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory Systems

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

One Health: the Interconnected Health of the Environment, Humans, and Other Animals

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