Question 1

Which of the following techniques is most commonly used in the prenatal diagnosis of genetic disorders, such as cystic fibrosis, in a human fetus?

Accepted Answer

A) amniocentesis 
B) chorionic villus sampling 
C) isolation of fetal cells from maternal blood 
A) amniocentesis 
B) chorionic villus sampling 
C) isolation of fetal cells from maternal blood A

Question 2

How were ancient peoples involved in biotechnology?

Accepted Answer

Ancient peoples practiced the selective breeding of plants and animals, which is a form of biotechnology.

Question 3

There are potential risks in performing recombinant DNA research on humans and plants. True or False?

Accepted Answer

A) True 
 B)False  True

Question 4

Restriction enzymes are "restricted" in the sense that they normally work on only bacterial DNA. True or False?

Accepted Answer

A) True 
 B)False

Question 5

What is the term for manipulating the molecular basis of inheritance via recombinant DNA technology?

Accepted Answer

A) Mendelian genetics 
B) biotechnology 
C) DNA fingerprinting 
D) restriction fragment length polymorphism (RFLP) 
E) the polymerase chain reaction (PCR) 
A) Mendelian genetics 
B) biotechnology 
C) DNA fingerprinting 
D) restriction fragment length polymorphism (RFLP) 
E) the polymerase chain reaction (PCR)

Question 6

Based on this figure, how many PCR cycles are necessary to produce 64 copies of the original DNA fragment being amplified?

Accepted Answer

A) one 
B) two 
C) three 
D) four 
E) six 
A) one 
B) two 
C) three 
D) four 
E) six

Question 7

Enzymes that cut double-stranded DNA at specific sites are called ________.

Accepted Answer

The answer of Enzymes that cut double-stranded DNA at specific...

Question 8

Compare and contrast the use of a DNA profile ("DNA fingerprinting")with regular ink fingerprinting.

Accepted Answer

Both have the ability to distinguish ind

Question 9

Which of the following is a valid scientific concern about the environment and transgenic crops?

Accepted Answer

A) Pollen from transgenic crops could carry the recombinant genes to wild relatives, with unpredictable consequences. 
B) Wild animals might eat the transgenic crop and become genetically mutated. 
C) Transgenic crops could escape cultivation and become "superweeds" that are incapable of being destroyed. 
D) The toxic products of the modified genes would be released to the environment when the plants die, resulting in drastic die-offs of local wildlife. 
A) Pollen from transgenic crops could carry the recombinant genes to wild relatives, with unpredictable consequences. 
B) Wild animals might eat the transgenic crop and become genetically mutated. 
C) Transgenic crops could escape cultivation and become "superweeds" that are incapable of being destroyed. 
D) The toxic products of the modified genes would be released to the environment when the plants die, resulting in drastic die-offs of local wildlife.

Question 10

Which of the following techniques amplifies a specific region of DNA?

Accepted Answer

A) PCR 
B) gel electrophoresis 
C) the use of DNA probes 
D) DNA profiles 
A) PCR 
B) gel electrophoresis 
C) the use of DNA probes 
D) DNA profiles

Question 11

If you want to develop a procedure for identifying a genetic disorder caused by 20 different alleles, what method would be most likely to be useful?

Accepted Answer

A) restriction fragment length polymorphisms (RFLPs) 
B) arrays of DNA probes on filter paper 
C) single tandem repeats (STRs) 
D) the polymerase chain reaction (PCR) 
E) gene therapy 
A) restriction fragment length polymorphisms (RFLPs) 
B) arrays of DNA probes on filter paper 
C) single tandem repeats (STRs) 
D) the polymerase chain reaction (PCR) 
E) gene therapy

Question 12

DNA recombination changes the genetic makeup of organisms. True or False?

Accepted Answer

A) True 
 B)False

Question 13

DNA moves within an electric field because it:

Accepted Answer

A) is positively charged. 
B) is negatively charged. 
C) makes up chromosomes, which are slightly electromagnetic. 
D) can possess different charges (positive or negative), depending on its base sequence. 
A) is positively charged. 
B) is negatively charged. 
C) makes up chromosomes, which are slightly electromagnetic. 
D) can possess different charges (positive or negative), depending on its base sequence.

Question 14

Which of the following is a valid difference between traditional (selective breeding)and modern (genetic engineering)forms of biotechnology?

Accepted Answer

A) Selective breeding can be done much more quickly than genetic engineering. 
B) Genetic engineering can use genes from unrelated species, whereas selective breeding must use genes from the same or very closely related individuals. 
C) Genetic engineering can alter the DNA sequence of entire populations, whereas selective breeding cannot. 
A) Selective breeding can be done much more quickly than genetic engineering. 
B) Genetic engineering can use genes from unrelated species, whereas selective breeding must use genes from the same or very closely related individuals. 
C) Genetic engineering can alter the DNA sequence of entire populations, whereas selective breeding cannot.

Question 15

Restriction enzymes have the ability to cut DNA at specific nucleotide sequences. True or False?

Accepted Answer

A) True 
 B)False

Question 16

All of the following have been produced by inserting human genes into bacteria EXCEPT:

Accepted Answer

A) growth hormone, which helps children grow normally. 
B) insulin, which treats diabetics. 
C) blood clotting enzymes, which treat hemophiliacs. 
D) cancer cell-killing hormones, which cure patients who have malignancies. 
E) clot-dissolving enzymes, which help heart attack victims recover. 
A) growth hormone, which helps children grow normally. 
B) insulin, which treats diabetics. 
C) blood clotting enzymes, which treat hemophiliacs. 
D) cancer cell-killing hormones, which cure patients who have malignancies. 
E) clot-dissolving enzymes, which help heart attack victims recover.

Question 17

Modern forensic biotechnology labs use sophisticated machines to analyze ________, such as D16, and to determine the number of specific genetic repeats in the samples.

Accepted Answer

A) RFLPs 
B) STRs 
C) primers 
D) PCRs 
A) RFLPs 
B) STRs 
C) primers 
D) PCRs

Question 18

Prenatal diagnosis is available for all of the following disorders EXCEPT:

Accepted Answer

A) cystic fibrosis. 
B) Down syndrome. 
C) sickle-cell anemia. 
D) AIDS. 
A) cystic fibrosis. 
B) Down syndrome. 
C) sickle-cell anemia. 
D) AIDS.

Question 19

Suppose ENZ-1 and ENZ-2 are two different restriction enzymes. If various pieces of DNA from different species are cut with these enzymes (as follows), which would most easily form recombinant molecules?

Accepted Answer

A) human DNA cut with ENZ-1 and gorilla DNA cut with ENZ-2 
B) human DNA cut with ENZ-1 and human DNA cut with ENZ-2 
C) human DNA cut with ENZ-2 and bacterial DNA cut with ENZ-2 
D) bacterial DNA cut with ENZ-1 and gorilla DNA cut with ENZ-2 
A) human DNA cut with ENZ-1 and gorilla DNA cut with ENZ-2 
B) human DNA cut with ENZ-1 and human DNA cut with ENZ-2 
C) human DNA cut with ENZ-2 and bacterial DNA cut with ENZ-2 
D) bacterial DNA cut with ENZ-1 and gorilla DNA cut with ENZ-2

Question 20

What is the advantage of using PCR over traditional bacterial cloning to generate large quantities of a specific piece of DNA?

Accepted Answer

PCR is faster and ch

Exam 13: Biotechnology

Which of the following techniques is most commonly used in the prenatal diagnosis of genetic disorders, such as cystic fibrosis, in a human fetus?

How were ancient peoples involved in biotechnology?

There are potential risks in performing recombinant DNA research on humans and plants. True or False?

Restriction enzymes are "restricted" in the sense that they normally work on only bacterial DNA. True or False?

What is the term for manipulating the molecular basis of inheritance via recombinant DNA technology?

Based on this figure, how many PCR cycles are necessary to produce 64 copies of the original DNA fragment being amplified?

Enzymes that cut double-stranded DNA at specific sites are called ________.

Compare and contrast the use of a DNA profile ("DNA fingerprinting")with regular ink fingerprinting.

Which of the following is a valid scientific concern about the environment and transgenic crops?

Which of the following techniques amplifies a specific region of DNA?

If you want to develop a procedure for identifying a genetic disorder caused by 20 different alleles, what method would be most likely to be useful?

DNA recombination changes the genetic makeup of organisms. True or False?

DNA moves within an electric field because it:

Which of the following is a valid difference between traditional (selective breeding)and modern (genetic engineering)forms of biotechnology?

Restriction enzymes have the ability to cut DNA at specific nucleotide sequences. True or False?

All of the following have been produced by inserting human genes into bacteria EXCEPT:

Modern forensic biotechnology labs use sophisticated machines to analyze ________, such as D16, and to determine the number of specific genetic repeats in the samples.

Prenatal diagnosis is available for all of the following disorders EXCEPT:

Suppose ENZ-1 and ENZ-2 are two different restriction enzymes. If various pieces of DNA from different species are cut with these enzymes (as follows), which would most easily form recombinant molecules?

What is the advantage of using PCR over traditional bacterial cloning to generate large quantities of a specific piece of DNA?

An Introduction to Life on Earth

Atoms, Molecules, and Life

Biological Molecules

Cell Structure and Function

Cell Membrane Structure and Function

Energy Flow in the Life of a Cell

Capturing Solar Energy: Photosynthesis

Harvesting Energy: Glycolysis and Cellular Respiration

The Continuity of Life: Cellular Reproduction

Patterns of Inheritance

Dna: the Molecule of Heredity

Gene Expression and Regulation

Principles of Evolution

How Populations Evolve

The Origin of Species

The History of Life

Systematics: Seeking Order Amidst Diversity

The Diversity of Prokaryotes and Viruses

The Diversity of Protists

The Diversity of Plants

The Diversity of Fungi

Animal Diversity I: Invertebrates

Animal Diversity Ii: Vertebrates

Animal Behavior

Population Growth and Regulation

Community Interactions

How Do Ecosystems Work

Earths Diverse Ecosystems

Conserving Earths Biodiversity

Homeostasis and the Organization of the Animal Body

Circulation

Respiration

Nutrition and Digestion

The Urinary System

Defenses Against Disease

Chemical Control of the Animal Body: the Endocrine System

The Nervous System

The Senses

Action and Support: the Muscles and Skeleton

Animal Reproduction

Animal Development

Plant Anatomy and Nutrient Transport

Plant Reproduction and Development

Plant Responses to the Environment

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