Question 1

Restriction enzymes are useful in recombinant DNA studies because they:

Accepted Answer

A) cut DNA only at specific locations. 
B) join the cut ends of small DNA molecules. 
C) can reproduce in bacteria. 
D) give plasmids antibiotic properties. 
E) can separate pieces of DNA and RNA from each other. 
A) cut DNA only at specific locations. 
B) join the cut ends of small DNA molecules. 
C) can reproduce in bacteria. 
D) give plasmids antibiotic properties. 
E) can separate pieces of DNA and RNA from each other.

Question 2

Specific fragments of DNA on a gel can be visualized using:

Accepted Answer

A) DNA probes. 
B) restriction enzymes. 
C) electrophoresis. 
D) single tandem repeats (STRs). 
E) a polymerase chain reaction (PCR). 
A) DNA probes. 
B) restriction enzymes. 
C) electrophoresis. 
D) single tandem repeats (STRs). 
E) a polymerase chain reaction (PCR).

Question 3

Which of the following involves the use of human genes?

Accepted Answer

A) herbicide-resistant corn 
B) Bt toxin production by soybeans 
C) banana-based vaccine against pathogenic E. coli 
D) growth hormonetreated cows 
E) insulin production by bacteria 
A) herbicide-resistant corn 
B) Bt toxin production by soybeans 
C) banana-based vaccine against pathogenic E. coli 
D) growth hormonetreated cows 
E) insulin production by bacteria

Question 4

The substances used to cut genes in recombinant DNA research are called:

Accepted Answer

A) DNA polymerases. 
B) RNA polymerases. 
C) spliceosomes. 
D) replicases. 
E) restriction enzymes. 
A) DNA polymerases. 
B) RNA polymerases. 
C) spliceosomes. 
D) replicases. 
E) restriction enzymes.

Question 5

Define the term "biotechnology."

Accepted Answer

Biotechnology is any genetic a

Question 6

Which of the following is NOT a natural means of DNA recombination?

Accepted Answer

A) sexual reproduction 
B) transformation in bacteria 
C) viral infection 
D) the polymerase chain reaction 
A) sexual reproduction 
B) transformation in bacteria 
C) viral infection 
D) the polymerase chain reaction

Question 7

At least 80% of the ________ grown in the United States is transgenic.

Accepted Answer

A) corn (only) 
B) cotton (only) 
C) soybeans (only) 
D) corn and soybeans (only) 
E) corn, cotton, and soybeans 
A) corn (only) 
B) cotton (only) 
C) soybeans (only) 
D) corn and soybeans (only) 
E) corn, cotton, and soybeans

Question 8

The genetic recombination of ________ caused massive, widespread epidemics that affected the global human population several times in the 20th century.

Accepted Answer

A) human and bacterial plasmids 
B) bacterial and viral plasmids 
C) bird flu viruses and human flu viruses 
D) HIV and flu viruses 
A) human and bacterial plasmids 
B) bacterial and viral plasmids 
C) bird flu viruses and human flu viruses 
D) HIV and flu viruses

Question 9

Based on this figure, to which of the following DNA sequences will the illustrated DNA probe bind?

Accepted Answer

A) AGATCTATCTATCTAGAT 
B) AGAAGATAGATAGATGAT 
C) AGAGATATGATAGATGAT 
D) AGAGATATCTATCTAGAT 
A) AGATCTATCTATCTAGAT 
B) AGAAGATAGATAGATGAT 
C) AGAGATATGATAGATGAT 
D) AGAGATATCTATCTAGAT

Question 10

Small, accessory chromosomes found in bacteria that are useful in recombinant DNA procedures are called:

Accepted Answer

A) plasmids. 
B) viroids. 
C) centrioles. 
D) bacteriophages. 
A) plasmids. 
B) viroids. 
C) centrioles. 
D) bacteriophages.

Question 11

Ligase enzymes are used to covalently bind together pieces of DNA. True or False?

Accepted Answer

A) True 
 B)False

Question 12

________ are small, circular pieces of DNA that are often used in the process of transformation.

Accepted Answer

The answer of ________ are small, circular pieces of DNA...

Question 13

In nature, DNA recombination occurs but it is random and undirected. True or False?

Accepted Answer

A) True 
 B)False

Question 14

In biotechnology research, DNA fragments created by restriction enzyme action are separated from one another via:

Accepted Answer

A) crossing over. 
B) gel electrophoresis. 
C) centrifugation. 
D) filtering. 
E) the polymerase chain reaction. 
A) crossing over. 
B) gel electrophoresis. 
C) centrifugation. 
D) filtering. 
E) the polymerase chain reaction.

Question 15

A recombinant plasmid contains only human and bacterial DNA. True or False?

Accepted Answer

A) True 
 B)False

Question 16

Provide one example of a medical application of biotechnology.

Accepted Answer

Possible answers are genetic s

Question 17

Which of the following can be used to detect genetic disorders?

Accepted Answer

A) restriction fragment length polymorphisms (RFLPs)only 
B) arrays of DNA probes only 
C) single tandem repeats (STRs)only 
D) RFLPs and DNA probe arrays only 
E) RFLPs, DNA probe arrays, and STRs 
A) restriction fragment length polymorphisms (RFLPs)only 
B) arrays of DNA probes only 
C) single tandem repeats (STRs)only 
D) RFLPs and DNA probe arrays only 
E) RFLPs, DNA probe arrays, and STRs

Question 18

In recombinant DNA technology, plasmids:

Accepted Answer

A) are used to insert foreign DNA into bacteria. 
B) show restriction enzymes where to cut bacterial DNA. 
C) are necessary for cellular respiration in certain plants. 
D) are the sites of protein synthesis in bacteria. 
E) are isolated from the blood of mammals. 
A) are used to insert foreign DNA into bacteria. 
B) show restriction enzymes where to cut bacterial DNA. 
C) are necessary for cellular respiration in certain plants. 
D) are the sites of protein synthesis in bacteria. 
E) are isolated from the blood of mammals.

Question 19

Which of the following is NOT a valid difference between traditional and modern forms of biotechnology?

Accepted Answer

A) Modern biotechnology can isolate and manipulate individual genes. 
B) Traditional biotechnology is almost always limited to genetic recombination between individuals of one species or two closely related species. 
C) Traditional biotechnology cannot produce entirely new genes in one step. 
D) Modern forms of biotechnology are slower than traditional forms of biotechnology. 
A) Modern biotechnology can isolate and manipulate individual genes. 
B) Traditional biotechnology is almost always limited to genetic recombination between individuals of one species or two closely related species. 
C) Traditional biotechnology cannot produce entirely new genes in one step. 
D) Modern forms of biotechnology are slower than traditional forms of biotechnology.

Question 20

If you cut out a gene using EcoRI and BamHI, what enzyme(s)will you need to insert the gene into another chromosome?

Accepted Answer

A) EcoRI only 
B) BamHI only 
C) both EcoRI and BamHI 
D) neither of these enzymes 
A) EcoRI only 
B) BamHI only 
C) both EcoRI and BamHI 
D) neither of these enzymes

Exam 13: Biotechnology

Restriction enzymes are useful in recombinant DNA studies because they:

Specific fragments of DNA on a gel can be visualized using:

Which of the following involves the use of human genes?

The substances used to cut genes in recombinant DNA research are called:

Define the term "biotechnology."

Which of the following is NOT a natural means of DNA recombination?

At least 80% of the ________ grown in the United States is transgenic.

The genetic recombination of ________ caused massive, widespread epidemics that affected the global human population several times in the 20th century.

Based on this figure, to which of the following DNA sequences will the illustrated DNA probe bind?

Small, accessory chromosomes found in bacteria that are useful in recombinant DNA procedures are called:

Ligase enzymes are used to covalently bind together pieces of DNA. True or False?

________ are small, circular pieces of DNA that are often used in the process of transformation.

In nature, DNA recombination occurs but it is random and undirected. True or False?

In biotechnology research, DNA fragments created by restriction enzyme action are separated from one another via:

A recombinant plasmid contains only human and bacterial DNA. True or False?

Provide one example of a medical application of biotechnology.

Which of the following can be used to detect genetic disorders?

In recombinant DNA technology, plasmids:

Which of the following is NOT a valid difference between traditional and modern forms of biotechnology?

If you cut out a gene using EcoRI and BamHI, what enzyme(s)will you need to insert the gene into another chromosome?

An Introduction to Life on Earth

Atoms, Molecules, and Life

Biological Molecules

Cell Structure and Function

Cell Membrane Structure and Function

Energy Flow in the Life of a Cell

Capturing Solar Energy: Photosynthesis

Harvesting Energy: Glycolysis and Cellular Respiration

The Continuity of Life: Cellular Reproduction

Patterns of Inheritance

Dna: the Molecule of Heredity

Gene Expression and Regulation

Principles of Evolution

How Populations Evolve

The Origin of Species

The History of Life

Systematics: Seeking Order Amidst Diversity

The Diversity of Prokaryotes and Viruses

The Diversity of Protists

The Diversity of Plants

The Diversity of Fungi

Animal Diversity I: Invertebrates

Animal Diversity Ii: Vertebrates

Animal Behavior

Population Growth and Regulation

Community Interactions

How Do Ecosystems Work

Earths Diverse Ecosystems

Conserving Earths Biodiversity

Homeostasis and the Organization of the Animal Body

Circulation

Respiration

Nutrition and Digestion

The Urinary System

Defenses Against Disease

Chemical Control of the Animal Body: the Endocrine System

The Nervous System

The Senses

Action and Support: the Muscles and Skeleton

Animal Reproduction

Animal Development

Plant Anatomy and Nutrient Transport

Plant Reproduction and Development

Plant Responses to the Environment

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